DOI . ORG {
}
We began analyzing https://link.springer.com/protocol/10.1007/978-1-60761-756-3_16, but it redirected us to https://link.springer.com/protocol/10.1007/978-1-60761-756-3_16. The analysis below is for the second page.
Title[redir]:
Measurement of Mitochondrial ROS Production | SpringerLink
Description:
The significance of reactive oxygen species (ROS) as aggravating or primary factors in numerous pathologies is widely recognized, with mitochondria being considered the major intracellular source of ROS. It is not yet possible to routinely measure mitochondrial ROS...
Title[redir]:
Measurement of Mitochondrial ROS Production | SpringerLink
Description:
The significance of reactive oxygen species (ROS) as aggravating or primary factors in numerous pathologies is widely recognized, with mitochondria being considered the major intracellular source of ROS. It is not yet possible to routinely measure mitochondrial ROS...
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Keywords {🔍}
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Topics {✒️}
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Questions {❓}
- Giorgio M, Trinei M, Migliaccio E, Pelicci PG (2007) Hydrogen peroxide: a metabolic by-product or a common mediator of ageing signals?
- ROS production by mitochondria: function or dysfunction?
- Sawyer DT, Valentine JS (1981) How super is superoxide?
Schema {🗺️}
ScholarlyArticle:
headline:Measurement of Mitochondrial ROS Production
pageEnd:255
pageStart:245
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60761-756-3.jpg
genre:
Springer Protocols
isPartOf:
name:Protein Misfolding and Cellular Stress in Disease and Aging
isbn:
978-1-60761-756-3
978-1-60761-755-6
type:Book
publisher:
name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Anatoly A. Starkov
affiliation:
name:Weill Medical College of Cornell University
address:
name:Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Reactive oxygen species, Mitochondria, Amplex, Oxidative stress
description:The significance of reactive oxygen species (ROS) as aggravating or primary factors in numerous pathologies is widely recognized, with mitochondria being considered the major intracellular source of ROS. It is not yet possible to routinely measure mitochondrial ROS in animals or cultured cells with a reasonable degree of certainty. However, at the level of isolated mitochondria, one can easily monitor and quantify the rate of ROS production, identify major sites of ROS production, and compare the rates of ROS production in mitochondria isolated from normal and diseased tissue. In this chapter, we describe in detail the most recent and reliable method to measure mitochondrial ROS as the rate of H2O2 emission. This method may be employed with minimal modifications to measure H2O2 production by mitochondria isolated from various tissues and under a wide variety of experimental conditions.
datePublished:2010
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isbn:
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name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
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name:Weill Medical College of Cornell University
address:
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name:Anatoly A. Starkov
affiliation:
name:Weill Medical College of Cornell University
address:
name:Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, USA
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