DOI . ORG {}

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We began analyzing https://link.springer.com/protocol/10.1007/978-1-4939-9236-2_8, but it redirected us to https://link.springer.com/protocol/10.1007/978-1-4939-9236-2_8. The analysis below is for the second page.

Title[redir]:
Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics | SpringerLink
Description:
Liquid chromatography coupled to mass spectrometry (LC-MS)-based metabolomics and lipidomics offers invaluable tools to qualitatively and quantitatively study biological systems. Historically, unbiased (or discovery) analysis has been performed independently of...

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Education
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🏙️ Massive Traffic: 50M - 100M visitors per month

Based on our best estimate, this website will receive around 80,904,851 visitors per month in the current month.

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Keywords {🔍}

pubmed, google, scholar, cas, metabolomics, mass, central, data, protocol, analysis, dalessandro, privacy, cookies, content, information, publish, spectrometrybased, nemkov, targeted, quantitative, springer, research, search, highthroughput, biological, online, methods, molecular, spectrometry, access, chapter, rev, anal, nat, download, usd, processing, personal, log, journal, untargeted, semitargeted, reisz, zheng, angelo, travis, part, book, assay, mol,

Topics {✒️}

ultra-high-field orbitrap analyzer month download article/chapter lc-ms-based targeted metabolomics high-throughput quantitative metabolomics mass spectrometry-based metabolomics semi-targeted lipid analysis bile acid-microbiota crosstalk high-throughput metabolomics privacy choices/manage cookies device instant download targeted metabolomics study high-performance quadrupole benchtop mass spectrometer quadrupole-orbitrap technology bile acid receptors targeted assay design metabolite extraction protocol web-based platform web-based inference integrative metabolomics analysis springer nature uplc-hr-ms quantitative tracing experiments central carbon european economic area multiple reaction monitoring improve analyte detection stable isotope dilution plant acyl lipids bioactive lipids accumulate nonlinear peak alignment subsequent statistical analyses editor information editors interactive xcms online journal finder publish conditions privacy policy alessandro & travis nemkov mass spectrometry accepting optional cookies post-transfusion circulation author information authors based metabolomics main content log check access ethics access protocol usd 49 quantitative metabolomics metabolomic data lc-ms chapter log

Schema {🗺️}

ScholarlyArticle:
      headline:Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics
      pageEnd:135
      pageStart:121
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-9236-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:High-Throughput Metabolomics
         isbn:
            978-1-4939-9236-2
            978-1-4939-9235-5
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Julie A. Reisz
            affiliation:
                  name:University of Colorado Denver
                  address:
                     name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Connie Zheng
            affiliation:
                  name:University of Colorado Denver
                  address:
                     name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Angelo D’Alessandro
            affiliation:
                  name:University of Colorado Denver
                  address:
                     name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Travis Nemkov
            affiliation:
                  name:University of Colorado Denver
                  address:
                     name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Untargeted metabolomics, Mass spectrometry, Oxylipids, Bile acids, Gradient, High-throughput, Absolute quantification
      description:Liquid chromatography coupled to mass spectrometry (LC-MS)-based metabolomics and lipidomics offers invaluable tools to qualitatively and quantitatively study biological systems. Historically, unbiased (or discovery) analysis has been performed independently of targeted, quantitative analysis such as multiple reaction monitoring (MRM). These practices have been aptly carried out based on technical limitations of each assay. The wide mass scanning ranges typical of discovery approaches limit assay sensitivity, while targeted methods that improve analyte detection do not acquire data on ions not included in the targeted assay design. Recent improvements to quadrupole-Orbitrap technology have improved both scan speed as well as sensitivity, thus making these instruments more robust. By combining the improved robustness and coverage with stable isotope dilution (SID) techniques, advantages of the separate assays can now be realized in a single run, thereby improving the throughput of this type of analysis.
      datePublished:2019
      isAccessibleForFree:
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Book:
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      isbn:
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         978-1-4939-9235-5
Organization:
      name:Springer New York
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         type:ImageObject
      name:University of Colorado Denver
      address:
         name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
         type:PostalAddress
      name:University of Colorado Denver
      address:
         name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
         type:PostalAddress
      name:University of Colorado Denver
      address:
         name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
         type:PostalAddress
      name:University of Colorado Denver
      address:
         name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
         type:PostalAddress
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Person:
      name:Julie A. Reisz
      affiliation:
            name:University of Colorado Denver
            address:
               name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
               type:PostalAddress
            type:Organization
      name:Connie Zheng
      affiliation:
            name:University of Colorado Denver
            address:
               name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
               type:PostalAddress
            type:Organization
      name:Angelo D’Alessandro
      affiliation:
            name:University of Colorado Denver
            address:
               name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
               type:PostalAddress
            type:Organization
      name:Travis Nemkov
      affiliation:
            name:University of Colorado Denver
            address:
               name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
      name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
      name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
      name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
WebPageElement:
      isAccessibleForFree:
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External Links {🔗}(131)

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