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Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics | SpringerLink
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Liquid chromatography coupled to mass spectrometry (LC-MS)-based metabolomics and lipidomics offers invaluable tools to qualitatively and quantitatively study biological systems. Historically, unbiased (or discovery) analysis has been performed independently of...
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Topics {✒️}
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Schema {🗺️}
ScholarlyArticle:
headline:Untargeted and Semi-targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics
pageEnd:135
pageStart:121
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-9236-2.jpg
genre:
Springer Protocols
isPartOf:
name:High-Throughput Metabolomics
isbn:
978-1-4939-9236-2
978-1-4939-9235-5
type:Book
publisher:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Julie A. Reisz
affiliation:
name:University of Colorado Denver
address:
name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
type:PostalAddress
type:Organization
type:Person
name:Connie Zheng
affiliation:
name:University of Colorado Denver
address:
name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
type:PostalAddress
type:Organization
type:Person
name:Angelo D’Alessandro
affiliation:
name:University of Colorado Denver
address:
name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
type:PostalAddress
type:Organization
type:Person
name:Travis Nemkov
affiliation:
name:University of Colorado Denver
address:
name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Untargeted metabolomics, Mass spectrometry, Oxylipids, Bile acids, Gradient, High-throughput, Absolute quantification
description:Liquid chromatography coupled to mass spectrometry (LC-MS)-based metabolomics and lipidomics offers invaluable tools to qualitatively and quantitatively study biological systems. Historically, unbiased (or discovery) analysis has been performed independently of targeted, quantitative analysis such as multiple reaction monitoring (MRM). These practices have been aptly carried out based on technical limitations of each assay. The wide mass scanning ranges typical of discovery approaches limit assay sensitivity, while targeted methods that improve analyte detection do not acquire data on ions not included in the targeted assay design. Recent improvements to quadrupole-Orbitrap technology have improved both scan speed as well as sensitivity, thus making these instruments more robust. By combining the improved robustness and coverage with stable isotope dilution (SID) techniques, advantages of the separate assays can now be realized in a single run, thereby improving the throughput of this type of analysis.
datePublished:2019
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name:University of Colorado Denver
address:
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type:PostalAddress
type:Organization
name:Connie Zheng
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address:
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name:Angelo D’Alessandro
affiliation:
name:University of Colorado Denver
address:
name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
type:PostalAddress
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name:Travis Nemkov
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address:
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name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
name:Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, USA
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