
DOI . ORG {
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Title[redir]:
Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System | SpringerLink
Description:
Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations is the key to successful development of drug or...
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Keywords {🔍}
cancer, cells, stem, article, google, scholar, pubmed, cell, cas, prostate, protocol, human, privacy, cookies, content, information, publish, research, isolation, regad, chapter, search, identification, line, zhou, download, university, springer, usd, personal, data, log, journal, system, buczek, reeder, tarik, book, methods, stemlike, access, tang, zhang, john, van, geest, nottingham, author, ebook, essential,
Topics {✒️}
nanog-egfp reporter system month download article/chapter spheroid body-forming cells nanog-gfp expression system cancer stem cells human colorectal cancer human ips cells cell line privacy choices/manage cookies xenograft human tumors device instant download nanog modulates stemness metastatic progenitor cells sphere-propagating cells cancer stem author information authors editor information editors prostate cancer european economic area eos lentiviral vectors mirnas expressed differently campania “luigi vanvitelli” journal finder publish conditions privacy policy cancer cells cross-functional network reeder & tarik regad accepting optional cookies cell sci 114 main content log protocol usd 49 nottingham trent university social media permissions reprints protocol cite cancer patients chapter log properties humana press immune cells tarik regad journal publish privacy policy check access ethics access protocol buczek uk magdalena books a optional cookies personal data
Questions {❓}
- Regad T (2017) Tissue-specific cancer stem cells: reality or a mirage?
- Wei C, Guomin W, Yujun L, Ruizhe Q (2007) Cancer stem-like cells in human prostate carcinoma cells DU145: the seeds of the cell line?
Schema {🗺️}
ScholarlyArticle:
headline:Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System
pageEnd:148
pageStart:139
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-7401-6.jpg
genre:
Springer Protocols
isPartOf:
name:Cancer Stem Cells
isbn:
978-1-4939-7401-6
978-1-4939-7400-9
type:Book
publisher:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Magdalena E. Buczek
affiliation:
name:Nottingham Trent University
address:
name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
type:PostalAddress
type:Organization
type:Person
name:Stephen P. Reeder
affiliation:
name:Nottingham Trent University
address:
name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
type:PostalAddress
type:Organization
type:Person
name:Tarik Regad
affiliation:
name:Nottingham Trent University
address:
name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Cancer stem cells (CSCs), Prostate cancer (PCa), NANOG, GFP reporter gene, Lentiviral expression system, Fluorescence-activated cell sorting, Sphere formation
description:Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations is the key to successful development of drug or immunotherapeutic assays. Here, we present a method of identification, isolation of Prostate Cancer Stem Cells (PCSCs) from the DU145 prostate cancer cell line using the NANOG-GFP expression system.
datePublished:2018
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address:
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name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
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