We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-7401-6_13.

Title:
Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System | SpringerLink
Description:
Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations is the key to successful development of drug or...
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

cancer, cells, stem, article, google, scholar, pubmed, cell, cas, prostate, protocol, human, privacy, cookies, content, information, publish, research, isolation, regad, chapter, search, identification, line, zhou, download, university, springer, usd, personal, data, log, journal, system, buczek, reeder, tarik, book, methods, stemlike, access, tang, zhang, john, van, geest, nottingham, author, ebook, essential,

Topics {✒️}

nanog-egfp reporter system month download article/chapter spheroid body-forming cells nanog-gfp expression system cancer stem cells human colorectal cancer human ips cells cell line privacy choices/manage cookies xenograft human tumors device instant download nanog modulates stemness metastatic progenitor cells sphere-propagating cells cancer stem author information authors editor information editors prostate cancer european economic area eos lentiviral vectors mirnas expressed differently campania “luigi vanvitelli” journal finder publish conditions privacy policy cancer cells cross-functional network reeder & tarik regad accepting optional cookies cell sci 114 main content log protocol usd 49 nottingham trent university social media permissions reprints protocol cite cancer patients chapter log properties humana press immune cells tarik regad journal publish privacy policy check access ethics access protocol buczek uk magdalena books a optional cookies personal data

Questions {❓}

Regad T (2017) Tissue-specific cancer stem cells: reality or a mirage?
Wei C, Guomin W, Yujun L, Ruizhe Q (2007) Cancer stem-like cells in human prostate carcinoma cells DU145: the seeds of the cell line?

Schema {🗺️}

ScholarlyArticle:
      headline:Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System
      pageEnd:148
      pageStart:139
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-7401-6.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Cancer Stem Cells
         isbn:
            978-1-4939-7401-6
            978-1-4939-7400-9
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Magdalena E. Buczek
            affiliation:
                  name:Nottingham Trent University
                  address:
                     name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Stephen P. Reeder
            affiliation:
                  name:Nottingham Trent University
                  address:
                     name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tarik Regad
            affiliation:
                  name:Nottingham Trent University
                  address:
                     name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Cancer stem cells (CSCs), Prostate cancer (PCa), NANOG, GFP reporter gene, Lentiviral expression system, Fluorescence-activated cell sorting, Sphere formation
      description:Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations is the key to successful development of drug or immunotherapeutic assays. Here, we present a method of identification, isolation of Prostate Cancer Stem Cells (PCSCs) from the DU145 prostate cancer cell line using the NANOG-GFP expression system.
      datePublished:2018
      isAccessibleForFree:
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         type:WebPageElement
      context:https://schema.org
Book:
      name:Cancer Stem Cells
      isbn:
         978-1-4939-7401-6
         978-1-4939-7400-9
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Nottingham Trent University
      address:
         name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
         type:PostalAddress
      name:Nottingham Trent University
      address:
         name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
         type:PostalAddress
      name:Nottingham Trent University
      address:
         name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Magdalena E. Buczek
      affiliation:
            name:Nottingham Trent University
            address:
               name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
               type:PostalAddress
            type:Organization
      name:Stephen P. Reeder
      affiliation:
            name:Nottingham Trent University
            address:
               name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
               type:PostalAddress
            type:Organization
      name:Tarik Regad
      affiliation:
            name:Nottingham Trent University
            address:
               name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
      name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
      name:The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

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