
NCBI . NLM . NIH . GOV {
}
Title[redir]:
TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer - PMC
Description:
RNA modifications are important regulatory elements of RNA functions. However, most genome-wide mapping of RNA modifications has focused on messenger RNAs and transfer RNAs, but such datasets have been lacking for small RNAs. Here we mapped ...
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Keywords {π}
doi, fig, rna, rnas, supplementary, pubmed, small, trfb, google, scholar, trfs, pmc, article, trf, free, mismatch, trmta, data, expression, analysis, trna, modification, rip, target, gene, cell, rnaseq, targets, trnas, protein, modifications, base, table, cancer, hekt, luciferase, reporter, fragments, enriched, seed, based, upr, sites, control, response, ago, sequences, macontaining, pairing, samples,
Topics {βοΈ}
pljm1-flag-ha-ago2-wt trans-blot semi-dry transfer interrupt regular watson-crick m1a4-modified trf-3b abolishes trf-3b gene-silencing activity ago-dependent gene-silencing mechanisms trmt6/61a-dependent m1a regulates peer review competing interests anti-m1a immuno-northern blot trans-blot turbo system rna-seq library preparation reverse-transcriptase-induced mismatch signature qiaseq qc spike-ins flag-ha-ago2 hek293t expressing unmodified trf-3004b tumor-free bladder lining pmc beta search dual-luciferase reporter assay tagged fh-ago2 protein trf-3-mediated gene silencing direct-zol rna miniprep acidic phenol-chloroform method ago2-bound trf-3b sequences tgirt template-switching activity12 dual-luciferase reporter assays stable fh-ago2 cells real-time quantitative pcr hrp-linked secondary antibodies high-resolution quantitative profiling ago-mediated gene silencing45 trmt6/61a-dependent m1a located m1a attenuates gene-silencing anti-pan-ago western blot footnotes publisher rna-induced silencing complex trf-3b prevents silencing trmt6/61a regulates upr 12% sds-page trmt6/61a regulates m1a activating pro-survival upr 3βmg/ml free 1-methyladenosine tgirt-seq identified trf-3s atf6-dependent promoter reporter biorad chemidoc xrsβ+βsystem improved tgirt-seq methods license information pmcid trmt6/61a promotes upr risc-mediated gene silencing18 rny4 y-rna fragment
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