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Title[redir]:
Presenilin mutations deregulate mitochondrial Ca2+ homeostasis and metabolic activity causing neurodegeneration in Caenorhabditis elegans - PMC
Description:
Mitochondrial dysfunction and subsequent metabolic deregulation is observed in neurodegenerative diseases and aging. Mutations in the presenilin (PSEN) encoding genes (PSEN1 and PSEN2) cause most cases of familial Alzheimerโs disease (AD); however, ...
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Keywords {๐}
mitochondrial, figure, animals, sel, doi, data, mutants, levels, type, wild, abeta, neuronal, pubmed, google, scholar, supplement, selty, ros, neurodegeneration, elegans, psen, activity, observed, function, elife, mitochondria, day, neurons, alm, mechanosensory, pmc, article, expressing, morphology, touch, quantification, response, fad, free, mutations, presenilin, disease, fibroblasts, ocr, source, cells, gammasecretase, raw, analysis, signaling,
Topics {โ๏ธ}
5 mg/ml 5-fluorour-aci1-2'-deoxyribose amyloid-beta-induced pathological behaviors funding statement pmc beta search er ca2+ย release improves funding information characteristic egg-laying defect beta-secretase recognition sequences crispr/cas9 mediated correction mediated gamma-secretase activity elife-33052-fig6-figsupp2-data2 editorial decision letter free radical generation reducing er ca2+release 5-trisphosphate-mediated calcium signaling mitochondrial ca2+concentration measurement elife-33052-fig6-figsupp1-data1 zhsex17 [pmec-4mitols normal c-terminal fragment visible gfp-labeled branch elife-33052-fig1-figsupp1-data1 elife-33052-fig3-figsupp1-data1 elife-33052-fig4-figsupp1-data1 elife-33052-fig5-figsupp1-data1 elife-33052-fig7-figsupp1-data1 elife-33052-fig8-figsupp1-data1 elife-33052-fig9-figsupp1-data1 accompanying author responses ros-mediated oxidative damage er-mitochondrial calcium handling co2 -free incubator ฮฑ-glycerophosphate dehydrogenase require gamma-secretase activity gamma-secretase mediated cleavage muscle-specific promoter fails mitochondria-targeted antioxidant suppresses org/mutations area-gomez 3xtg-ad mouse model license information pmcid additional null allele gamma-secretase dead sel-12 oxygen consumption rate ratiometric gcamp6/mcherry construct seahorse xfp analyzer measuring oxygen consumption h2dcf-da assay alzheimer's-linked mutation reactive oxygen species ฮณ-secretase independent role mitochondrial matrix ca2+
Questions {โ}
- 1) Does ETC respiration play a direct role on the phenotypes observed?
- 5-30 ฮผg/ml range) have been shown to affect mitochondrial function in cells, while still not affecting viability, why is such a high amount used here?
- How could the authors reconcile their proposed mechanism with this work?
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