
NCBI . NLM . NIH . GOV {
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Title[redir]:
ALKBH1 is an RNA dioxygenase responsible for cytoplasmic and mitochondrial tRNA modifications - PMC
Description:
ALKBH1 is a 2-oxoglutarate- and Fe2+-dependent dioxygenase responsible for multiple cellular functions. Here, we show that ALKBH1 is involved in biogenesis of 5-hydroxymethyl-2΄-O-methylcytidine (hm5Cm) and 5-formyl-2΄-O-methylcytidine (f5Cm) at the ...
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Keywords {🔍}
alkbh, cells, google, scholar, pubmed, doi, mitochondrial, mttrnamet, nsun, pmc, hmc, rna, figure, article, free, trna, formation, cttrnaleucaa, fcm, protein, form, human, hmcm, trnas, modification, vitro, position, supplementary, isolated, mutations, ftsj, modifications, cell, fragments, involved, biogenesis, found, translation, indicating, demethylation, activity, wobble, oxidation, fragment, responsible, detected, dna, hekt, lines, mass,
Topics {✒️}
10 μm isopropyl β-d-1-thiogalactopyranoside his6-sumo-tagged human alkbh1 autosomal-recessive intellectual disability canonical watson–crick geometry proto-spacer adjacent motif pmc beta search l-cys-free medium sex-ratio distortion ribosomal a-site revealed 2΄-o-methylation takes place alkbh1-knockout cells exhibited s-adenosylmethionine-binding protein sole mt-trnamet acts post-spliced trna precursor modulating codon–anticodon interaction [google scholar] 3 [google scholar] 4 [google scholar] 15 crispr-cas9 system basically repairs alkylation damage yield pe-sumo-alkbh1 dna alkylation damage fe2+-dependent rna dioxygenase fe2+-dependent dioxygenase responsible fe2+/2-og-dependent oxygenases vitro transcribed mt-trnamet fine-tuning decoding properties alkbh1-mediated f5c34 formation nsun3-mediated m5c34 formation ribosomal a-site introduced plasmid-encoded alkbh1 negative ions derived his6-sumo tag rnase t1–digested fragments f5c34 enables mt-trnamet mutant mt-trnasmet normalized anticodon–stem–loop region transcriptome-wide distribution reciprocal circulating chromatography supplementary materials included [35s] met/[35s] cys supplementary table s1 supplementary table s2 license information pmcid nucleic acids res isolated mt-trnamet revealed mouse fetus ct-trnasleu post-transcriptional modifications publisher site pdf trm4/nsun2 methylates c34
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