
NCBI . NLM . NIH . GOV {
}
Title[redir]:
tRNA base methylation identification and quantification via high-throughput sequencing - PMC
Description:
Eukaryotic transfer RNAs contain on average 14 modifications. Investigations of their biological functions require the determination of the modification sites and the dynamic variations of the modification fraction. Base methylation represents a ...
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Keywords {π}
trna, modification, modifications, trnas, rna, fig, google, scholar, pubmed, doi, stop, demethylase, pmc, sequencing, article, sites, free, modified, treatment, stops, human, methylations, rrna, type, base, values, position, supplemental, site, data, reads, mutation, high, reaction, dmtrnaseq, mitochondrial, fraction, aspgtc, methylation, fractions, method, mutations, shown, quantitative, present, primer, extension, leucag, bowtie, cells,
Topics {βοΈ}
high-throughput dm-trna-seq data published dm-trna-seq results [google scholar] grosjean methyl-2-thio-n6-threonylcarbamoyl-adenosine methyl-2-thio-n6-isopentenyl-adenosine results establish dm-trna-seq pmc beta search 5-methoxy-carbonyl-methyl-2-thio trm9-catalyzed trna modifications zorbax eclipse xdb-c18 genome-wide protein coding discovering rna-protein interactome methods dm-trna-seq high-throughput sequencing wesley reverse transcription-based methods Ξ±-32p-dntp incorporated dm-trna-seq data cell-state-dependent pattern divalent metal ions dm-trna-seq method org/cgi/doi/10 dm-rna-seq method dynamic n-methyladenosine methylome respective Ξ±-32p-dntp reverse transcription-based method dynamic n1-methyladenosine methylome applying dm-trna-seq supplementary materials included enable transcriptome-wide analysis demethylase-treated samples show qqq lc-ms showed dm-trna-seq open post-transcriptional rna modifications references occurred simultaneously demethylase-treated sequencing data n1-methyl-adenosine modification hydrazine/aniline treatment yielded rapid resolution ht codon-biased translation rnaβcdna structure effects m3c-specific base chemistry dm-trna-seq perform structural roles dm-rna-seq dynamic ranges exist custom-made trna microarrays high-throughput method standard quality control high-throughput annotation high-throughput sequencing
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