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Keywords {🔍}

pkm, cells, acetylation, doi, figure, protein, cell, hsc, pubmed, google, scholar, activity, glucose, level, mutant, western, pcaf, degradation, binding, acetylated, cancer, pmc, tumor, levels, blot, pyruvate, growth, cma, article, interaction, treatment, tsa, determined, kinase, lysosomes, regulation, proteins, nam, decreases, decreased, free, promotes, enzyme, high, glycolytic, increased, antibody, endogenous, wildtype, expression,

Topics {✒️}

pkm2-q393a/l394a mutant pmc beta search fluoro-2-deoxy-d-glucose flag-tagged wild-type tables nihms784975-supplement-document_s1__five_figures_and_two_tables_ lc-ms/ms analysis pancreatic beta-cell line original stable h1299/pkm2 degrades short-lived proteins q223a/d224a mutation decreased lacks acetylation-induced inhibition vector expressing flag-pkm2 glucose-induced pkm2 degradation mol cell summary h1299/pkm2k305q-expressing cells promotes lysosome-dependent degradation high glucose-induced decrease h1299/pkm2k305q cells proliferated h1299 cells wild-type accumulates glycolytic intermediates h1299/pkm2k305q cells accumulated cma-mediated protein degradation higher-order chromatin structure acetyl mimetic substitution nadp/nadph assay kit enriched k305-acetylated pkm2 increases pk activity anti-acetyllysine antibody confirmed lc-ms/ms accumulate glycolytic intermediates h1299/pkm2k305q pools supplementary materials included acetylation mimetic mutation ectopically expressing pcaf mutant enzyme activity promotes cell proliferation h1299/pkm2k305r cells accumulated major acetylation site decreased pk activity license information pmcid mass spectrometric analyses short hairpin rna glucose-induced acetylation k305q mutant displayed k305q mutation decreases histone deacetylase hdac flag-tagged pkm2 high glucose promotes k305q mutant pkm2 lamp2a knockdown blocks

Questions {❓}

• What is the physiological significance of this glucose-dependent regulation in cancer cells?

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• What's the financial outcome of https://scholar.google.com/scholar_lookup?journal=Biochem%20J&title=Proteins%20containing%20peptide%20sequences%20related%20to%20KFERQ%20are%20selectively%20depleted%20in%20liver%20and%20heart,%20but%20notskeletal%20muscle,%20of%20fasted%20rats&author=S%20Wing&author=HL%20Chiang&author=AL%20Goldberg&author=JF%20Dice&volume=275&publication_year=1991&pages=165-169&pmid=2018472&doi=10.1042/bj2750165&?
• What's the financial outcome of https://doi.org/10.1126/science.1166088?
• Income figures for https://scholar.google.com/scholar_lookup?journal=Science&title=Regulation%20of%20neuronal%20survival%20factor%20MEF2D%20by%20chaperone-mediated%20autophagy&author=Q%20Yang&author=H%20She&author=M%20Gearing&author=E%20Colla&author=M%20Lee&volume=323&publication_year=2009&pages=124-127&pmid=19119233&doi=10.1126/science.1166088&
• How much does https://doi.org/10.1093/emboj/17.11.3155 pull in?
• https://scholar.google.com/scholar_lookup?journal=EMBO%20J&title=Essential%20and%20redundant%20functions%20of%20histone%20acetylation%20revealed%20by%20mutation%20of%20target%20lysines%20and%20loss%20of%20the%20Gcn5p%20acetyltransferase&author=W%20Zhang&author=JR%20Bone&author=DG%20Edmondson&author=BM%20Turner&author=SY%20Roth&volume=17&publication_year=1998&pages=3155-3167&pmid=9606197&doi=10.1093/emboj/17.11.3155& income
• Revenue of https://doi.org/10.1126/science.1179689
• How much income is https://scholar.google.com/scholar_lookup?journal=Science&title=Regulation%20of%20cellular%20metabolism%20by%20protein%20lysine%20acetylation&author=S%20Zhao&author=W%20Xu&author=W%20Jiang&author=W%20Yu&author=Y%20Lin&volume=327&publication_year=2010&pages=1000-1004&pmid=20167786&doi=10.1126/science.1179689& earning monthly?
• https://github.com/ncbi's total income per month
• https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/%4038.9959508, -77.101021,17z/data%3D!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb%3A0x19156f88b27635b8!8m2!3d38.9959508! 4d-77.0988323's total income per month
• How much does https://www.nlm.nih.gov/web_policies.html net monthly?
• How much revenue does https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office produce monthly?
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• See how much https://www.nlm.nih.gov/careers/careers.html makes per month
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• https://www.nih.gov/'s total income per month
• What's the profit of https://www.hhs.gov/?
• How much income does https://www.usa.gov/ have?

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