
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Modulation of deregulated chaperone-mediated autophagy by a phosphopeptide - PMC
Description:
The P140 peptide, a 21-mer linear peptide (sequence 131–151) generated from the spliceosomal SNRNP70/U1–70K protein, contains a phosphoserine residue at position 140. It significantly ameliorates clinical manifestations in autoimmune patients with ...
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Keywords {🔍}
cells, peptide, cma, hspa, lysosomal, mrllpr, mice, lysosomes, doi, pmid, fig, pubmed, google, scholar, httpdxdoiorg, effect, lampa, cell, protein, activity, pmc, article, lupus, proteins, autophagy, free, observed, pathway, gapdh, incubated, experiments, results, binding, hspaa, lumenal, splenocytes, expression, substrate, fluor, purified, muller, lumen, levels, peptides, alexa, degradation, min, values, green, inhibitory,
Topics {✒️}
biotin-coupled anti-goat antibody allophycocyanin-labeled anti-cd19 antibody mrl/lpr lupus-prone mice1 spliceosomal snrnp70/u1–70k protein clathrin-dependent endo-lysosomal pathway mrl/lpr lupus-prone mice improm-ii reverse transcriptase methoxycarbonyl solid-phase chemistry high-content microscopy allowing liquid chromatography-mass spectrometry autoimmune mrl-lpr/lpr mice spliceosomal snrnp70/u1–70k serum-free medium supplemented p140-treated mrl/lpr mice spliceosomal autoantigen u1-70k pmc beta search hsp40-stimulated atpase activities h-2db-restricted cd8+ lysosensor yellow/blue dnd-160 alzheimer-related ps1 mutations peroxidase-conjugated secondary antibody anti-dna antibody production disrupt caveolae-dependent endocytosis sick mrl/lpr mice unprimed mrl/lpr mice peptide dose-dependent manner anti-hspa8 monoclonal antibody serum-free medium enzyme-linked immunosorbent assay untreated mrl/lpr mice energy-dependent cellular process clathrin-dependent endosomal pathway blue/yellow fluorescent ratio lysosensor yellow/blue revealed mrl/lpr mice exhibit track chaperone-mediated autophagy anti-hspa8 antibody 1b5 blue/yellow fluorescence ratio serum-free conditions mrl/lpr lupus mice healthy age-matched cba/ plastic plate-immobilized p140 real-time biodistribution experiments mrl/lpr extracts due peptide concentration-dependent manner autophagy markers sqstm1/p62 5 mm methyl-β-cyclodextrin counter-balance p140 inhibition cma-targeting motif kferq substrate-chaperone complex binds
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