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Keywords {🔍}

doi, boutons, synaptic, agrp, neurons, google, scholar, fig, pubmed, pomc, cell, pvh, synapses, circuit, pmc, vesicles, release, analysis, mouse, neuron, article, types, electron, defined, nonsynaptic, free, labeling, mice, molecularly, supplementary, postsynaptic, labeled, axonal, sites, circuits, arcagrppvh, viral, projections, vesicle, vamphrp, expression, axons, contact, ultrastructural, hrp, volume, neural, microscopy, membrane, dab,

Topics {✒️}

raav2/1-cag-flex-rev-chr2 raav2/1-cag-flex-rev-vamp2 raav2-cag-flex-rev-vamp2 hsv129-loxp-stop-loxp-tdtomato dab-reaction product electron-dense raav2-cag-flex backbone tannic acid-glutaraldehyde-saponin fixation α-melanocyte stimulating hormone alpha-melanocyte-stimulating hormone multi-synapse arcagrp→pvh connections channelrhodopsin-2-assisted circuit mapping long-range callosal projections visualize long-range connectivity long-range circuit mapping high-throughput cre reporting guinea pig anti-vglut2 cell type-specific connectomics pathway-specific feedforward circuits cell type-specific connectivity long-range axonal projections loxp-stop-loxp-tdtomato vesicular glutamate transporter-2-positive guinea pig anti-rfp24 cell type-specific marker cell type-specific labeling cell type-specific connections long-range ultrastructure mapping prevents fasting-induced suppression hrp enables high-resolution cre-dependent stable inversion pmc beta search gesem-labeling enables systematic cell type-specific ultrastructure key long-range connections arcpomc→pvh projections suggests arcagrp→pvh projections showed primary antibodies diluted cre-dependent viral vector melanocortin receptor-independent pathway24 prior immuno-em analysis29 hrp-mediated dab oxidation ferrocyanide-reduced osmium methods h129δtk-tt viral vector gal4-expressing cell types mann-whitney u-test identifiable post-synaptic partner multiple gesem-labeled boutons trans-synaptic viral tracing5 hypothalamic arcuate nucleus immunohistochemical/light microscopic investigation

Questions {❓}

• Do synaptic and non-synaptic types of boutons arise from separate neuronal subpopulations?

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