
NCBI . NLM . NIH . GOV {
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Title[redir]:
Ribosome Profiling Reveals Pervasive Translation Outside of Annotated Protein-Coding Genes - PMC
Description:
Ribosome profiling suggests that ribosomes occupy many regions of the transcriptome thought to be non-coding, including 5′ UTRs and lncRNAs. Apparent ribosome footprints outside of protein-coding regions raise the possibility of artifacts unrelated ...
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Keywords {🔍}
ribosome, doi, footprints, translation, reading, sequences, profiling, google, scholar, pubmed, coding, rna, noncoding, ribosomes, fragments, figure, frames, pmc, length, article, rnas, lncrnas, transcript, free, data, cell, fragment, human, background, yeast, proteincoding, analysis, annotated, genes, true, aug, transcripts, frame, protein, cells, regions, ingolia, utrs, proteins, codons, affinity, floss, science, initiation, distribution,
Topics {✒️}
nonsense-mediated mrna decay [google scholar] ulitsky rabbit anti-hexokinase antibody guide pre-rrna modification science post-doctoral fellowship lineage-specific polysome isolation mouse anti-gfp antibody exert cis-acting effects emetine-versus cycloheximide-treated cells c-terminal gfp fusions supplementary materials included ordinary protein-coding genes pmc beta search annotated protein-coding genes yield cancer-specific antigens human protein-coding genes cycloheximide-stabilized yeast polysomes protein-coding reading frame annotated protein-coding sequences nuclear protein-coding genes conserved protein-coding genes protein-coding regions raise multi-small-nucleolar-rna annotated protein-coding regions aug-initiated reading frame clear protein-coding potential ribosome-protected mrna fragments sophisticated conservation-based metrics aug-initiated reading frames perez-ortin references aitken ce fully-spliced transcript shown cycloheximide-treated yeast polysomes streptavidin-coated magnetic beads secretion signal sequence genome-wide translational profiling ribosome profiling-derived reads chromatin signature reveals human-derived background reads mouse embryonic stem lin mf schneider-poetsch cumulative length distribution discrete reading frame ribosome fooptirnt organization cellular immune system license information pmcid remove rna-binding proteins streptavidin-bound sample relative long noncoding rna
Questions {❓}
- Execution of nonsense-mediated mRNA decay: what defines a substrate?
- The broad implications of pervasive translation and the discrepancy between ribosome profiling and conservation analysis pose an immediate question: do the footprint sequences detected in these profiling experiments indicate the presence of assembled (80S) ribosomes?
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