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We began analyzing https://link.springer.com/protocol/10.1007/978-1-59745-232-8_5, but it redirected us to https://link.springer.com/protocol/10.1007/978-1-59745-232-8_5. The analysis below is for the second page.

Title[redir]:
Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases | SpringerLink
Description:
Zinc-finger nucleases (ZFNs) are promising new tools for enhancing the efficiency of gene targeting in many organisms. Because of the flexibility of zinc finger DNA recognition, ZFNs can be designed to bind many different genomic sequences. The double-strand breaks...

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Education
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Keywords {🔍}

google, scholar, pubmed, cas, article, gene, nucleases, zinc, finger, drosophila, carroll, cold, spring, harbor, targeting, zincfinger, dna, press, laboratory, cleavage, mutagenesis, elegans, beumer, trautman, doublestrand, homologous, recombination, targeted, golic, cells, segal, mol, proc, natl, acad, sci, usa, biol, privacy, cookies, content, information, publish, protocol, caenorhabditis, morton, designed, domains, bibikova, search,

Topics {✒️}

double-strand-break-induced homologous recombination double-strand dna break designer zinc-finger proteins custom zinc-finger nucleases custom dna-binding domains double-strand break repair designed zinc-finger nucleases custom-designed molecular scissors high-frequency homologous recombination double-strand breaks double-strand cleavage cold spring harbor zinc finger nucleases zinc-finger nucleases zinc finger domains targeted chromosomal cleavage privacy choices/manage cookies zinc finger fusions efficient gene targeting enhancing gene targeting dna doublestrand breaks zinc finger tools exogenous donor dna sigma-aldrich corporation targeted gene knockout step gene disruption controlling gene expression gene conversion tracts chimeric restriction enzymes journal finder publish european economic area nucleic acids res hybrid restriction enzymes plasmid-borne sequence salt lake city foki cleavage domain mammalian gene targeting protocol cite humana press conditions privacy policy artificial transcription factors targeted gene replacement ana bozas & jonathan chromosomal mutagenesis accepting optional cookies nematode caenorhabditis elegans main content log dna cleavage drosophila female germline chapter log

Schema {🗺️}

ScholarlyArticle:
      headline:Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases
      pageEnd:77
      pageStart:63
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-59745-232-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Chromosomal Mutagenesis
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      author:
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            name:Kelly J. Beumer
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      keywords:Caenorhabditis elegans, DNA repair, Drosophila, fruit flies, gene targeting, homologous recombination, mutagenesis, nematodes, nonhomologous end joining, zinc finger, zinc-finger nuclease
      description:Zinc-finger nucleases (ZFNs) are promising new tools for enhancing the efficiency of gene targeting in many organisms. Because of the flexibility of zinc finger DNA recognition, ZFNs can be designed to bind many different genomic sequences. The double-strand breaks they create are repaired by cellular processes that generate new mutations at the cleavage site. In addition, the breaks can be repaired by homologous recombination with an exogenous donor DNA, allowing the experimenter to introduce designed sequence alterations. We describe the construction of ZFNs for novel targets and their application to targeted mutagenesis and targeted gene replacement in Drosophila melanogaster and Caenorhabditis elegans.
      datePublished:2008
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      name:Kelly J. Beumer
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      name:J. Jason Morton
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      name:Ana Bozas
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