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Title[redir]:
Characterization of the human tRNA-guanine transglycosylase: Confirmation of the heterodimeric subunit structure - PMC
Description:
The eukaryotic tRNA-guanine transglycosylase (TGT) has been reported to exist as a heterodimer, in contrast to the homodimeric eubacterial TGT. While ubiquitin-specific protease 14 (USP14) has been proposed to act as a regulatory subunit of the ...
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Keywords {🔍}
tgt, human, queuine, transglycosylase, hqtrtd, protein, pubmed, google, scholar, coli, hqtrt, activity, doi, hthqtrt, trnatyr, heterodimer, trna, kda, hthqtrthqtrtd, usp, subunit, guanine, cells, trnaguanine, qtrt, kinetic, enzyme, buffer, mass, gene, sdspage, pmc, qtrtd, min, free, purified, katze, shown, observed, studies, subunits, purification, garcia, article, reported, salvage, heterodimeric, eukaryotic, chromatography, proteins,
Topics {✒️}
matrix-assisted laser desorption/ionization wild-type pht-hqtrt1·hqtrtd1 wild-type ht-hqtrt1•hqtrtd1 higher-molecular-weight protein band wild-type ht-hqtrt1·qtrtd1 5-cis-dihydroxy-2-cyclopenten-1-yl cary uv-visible spectrophotometer 5-cis-dihydroxy-1-cyclopenten-3-ylaminomethyl cross-linked ht-hqtrt1·hqtrtd1 n-terminal polyhistidine-tagged proteins pmc beta search ht-hqtrt1•hqtrtd1 cross-linking covalent rna–tgt intermediate cross-linked protein band site-directed mutagenesis confirms quikchange site-directed mutagenesis �free” ht-hqtrt1 monomer wild-type human tgt low-molecular-weight resolution mitochondria-localized heteromeric transglycosylase purified ht-hqtrt1·hqtrtd1 complex dms-treated ht-hqtrt1•hqtrtd1 ni2+-nta wash buffer low-melting seaplaque agarose site-directed mutagenesis studies low-molecular-weight standards sodium dodecyl sulfate �queuine trna-ribosyltransferase domain queuine trna-ribosyltransferase domain polyhistidine-tagged human qtrt1 tgt active-site residues 2% agarose- tris-acetate-edta ni2+-nta bind buffer cross-linked tgt heterodimer germ-free mice polyacrylamide gel electrophoresis queuine-lacking transfer rnas chemical cross-linking confirm transfer rna-guanine ribosyltransferase ubiquitin-specific protease family aminomethane hydrochloride [tris-hcl] generates free queuine produce free queuine wild-type enzyme human trna-guanine transglycosylase coli trna-guanine transglycosylase bisimidoester cross-linker org/cgi/doi/10 plasmid pht-hqtrt1·hqtrtd1 eukaryotic trna-guanine transglycosylase
Questions {❓}
- Transglycosylation: A mechanism for RNA modification (and editing?
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