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Title[redir]:
Estrogen-Related Receptor Beta Interacts with Oct4 To Positively Regulate Nanog Gene Expression - PMC
Description:
Embryonic stem (ES) cell self-renewal is regulated by transcription factors, including Oct4, Sox2, and Nanog. A number of additional transcriptional regulators of ES cell self-renewal have recently been identified, including the orphan nuclear ...
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Keywords {🔍}
esrrb, nanog, oct, cells, cell, expression, promoter, fig, octsox, binding, google, scholar, doi, pubmed, protein, sox, erre, site, levels, control, gene, analysis, chip, antibodies, stem, transcription, dna, antibody, shrna, regulation, experiments, error, pmc, activity, embryonic, selfrenewal, differentiation, zhbtc, element, genes, complex, independent, receptor, shown, doxycycline, luciferase, mol, nuclear, regulates, foct,
Topics {✒️}
estrogen-related receptor beta frt-ires-pac-frt cassette estrogen-related receptor family dual cross-linked chromatin cross-linking agent confirms step cross-linking method dual cross-linking chip pmc beta search empty vector pgl-basic ltq-orbitrap mass spectrometer nano-electrospray mass spectrometry fbxl10/jhdm1b histone demethylase puromycin-sensitive tng-ps cells estrogen-related receptors estrogen-related receptors 1 protein-protein interactions facilitated composite oct-sox site protein g-sepharose beads nf-kappab gene network rnai constructs psuper-esrrb-sh1 oct4-interacting partner protein downstream oct-sox element ring1b/rnf2 interactors identifies genome-wide chromatin immunoprecipitation pgl-basic control vector silver-stained sds-paa gel oct-sox site compared orphan nuclear receptors flag-esrrb-transfected es cells tng-ps es cells xx-chip analysis showed oct-sox site sequence support esrrb-mediated transcription esrrb xx-chip shows formaldehyde cross-linking band representing flag-oct4 bacterially expressed gst-oct4 protein-protein interactions f-oct4 es cells transcription factor interactions positively regulate transcription oct-sox target genes nanog promoter-luciferase construct bcl6 interacting corepressor nanog-mediated gene regulation medical research council pnanog-luc constructs 50 μm beta-mercaptoethanol figure 3e shows f-oct4 extracts identified
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