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We began analyzing https://www.nature.com/articles/379466a0, but it redirected us to https://www.nature.com/articles/379466a0. The analysis below is for the second page.

Title[redir]:
Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry | Nature
Description:
MOLECULAR analysis of complex biological structures and processes increasingly requires sensitive methods for protein sequencing. Electrospray mass spectrometry1 has been applied to the high-sensitivity sequencing of short peptides2, but technical difficulties have prevented similar success with gel-isolated proteins. Here we report a simple and robust technique for the sequencing of proteins isolated by polyacrylamide gel electro-phoresis, using nano-electrospray3,4 tandem mass spectrometry5,6. As little as 5ng protein starting material on Coomassie- or silver-stained gels can be sequenced. Multiple-sequence stretches of up to 16 amino acids are obtained, which identify the protein unambiguously if already present in databases or provide information to clone the corresponding gene. We have applied this method to the sequencing and cloning of a protein which inhibits the proliferation of capillary endothelial cells in vitroand thus may have potential antiangiogenic effects on solid tumours.

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article, nature, google, scholar, cas, protein, access, ads, content, sequencing, mann, cookies, mass, wilm, privacy, matthias, fotsis, peptide, research, data, information, journal, proteins, spectrometry, open, analyt, advertising, subscribe, polyacrylamide, gels, nanoelectrospray, shevchenko, houthaeve, lothar, schweigerer, theodore, present, institution, buy, science, sci, author, university, germany, heidelberg, permissions, communications, optional, analysis, media,

Topics {✒️}

nature portfolio permissions reprints privacy policy peptide research advertising social media subscribe nature nature 368 nature 310 nature 379 nature nano-electrospray mass spectrometry apoptosis-inducing active protein polyacrylamide gel electro-phoresis lothar schweigerer personal data springerlink instant access data protection permissions capillary endothelial cells electrospray mass spectrometry1 prevented similar success privacy article wilm chip protein separation complex biological structures explore content subscription content high-sensitivity sequencing european economic area multiple-sequence stretches potential antiangiogenic effects institutional subscriptions read generative ai guided oncogenic kras-induced accepting optional cookies journals search log issue learn silver-stained gels manage preferences loannina medical school article purchase gel-isolated proteins usage analysis content article cite access protein sequencing present provide information

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