
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Optimal induction of T helper 17 cells in humans requires T cell receptor ligation in the context of Toll-like receptor-activated monocytes - PMC
Description:
Recently, a new lineage of CD4+ T cells has been described in the mouse that specifically secretes IL-17 [T helper (Th) 17]. This discovery has led to a revision of the hypothesis that many autoimmune diseases are predominantly a Th1 phenomenon and ...
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Keywords {🔍}
cells, doi, monocytes, cell, presence, google, scholar, pubmed, fig, tregs, induction, data, memory, human, ifnγ, shown, conditions, addition, humans, percentage, immunol, pmc, lps, anticd, absence, cultured, production, article, naïve, tgfβ, stimulated, expression, skewing, mab, ngml, activated, cdcdfoxp, free, population, days, experiments, contact, increase, found, effects, hril, cytokine, generation, observed, average,
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org/cgi/content/full/0708426104/dc1 cfse/ddao-labeled cd4+cd45ro+cd25−foxp3− ddao-labeled cd4+cd25−foxp3− effector ddao-labeled cd4+cd25+foxp3+ tregs cd4+cd25+foxp3+ tregs promote immobilized anti-cd3/cd28 mabs functional cd4+cd25+foxp3+ tregs anti-cd3/cd28 mab stimulation cd4+cd25+foxp3+ treg population nonparametric matched-pairs test cd4+cd25+foxp3+ tregs suppressed ifn-γ-producing th1 cells pmc beta search medical research council efficiently suppress ifn-γ long-term repetitive stimulation short-term culture period plate-bound anti-cd3 mj research/bio-rad apc-free stimulation cells cd4+cd25+foxp3+ tregs anti-ifn-γ mab anti-ifn-γ fitc fitc-labeled ifn-γ anti-cd8-pe-cy5 anti-cd3/cd28 microbeads monocyte/macrophage-derived cytokines antigen-presenting cells ifn-γ-producing cells anti-cd14-pe-cy5 cd4+cd25−cd45ro+ responder apc-free environment lineage-defining transcription factors apc-free system supplementary materials included receptor-activated monocytes hayley ifn-γ+/il-17+ population additional human recombinant double positive cd4+ induce il-17-producing cells 100 ng/ml anti-cd3 monocyte-free system supporting information online anti-cd28 mab tgf-β dramatically reduced anti-cd25-pe th2 skewing significantly anti-ifn-γ reaction mix consisting red dye ddao
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