
NCBI . NLM . NIH . GOV {
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Title[redir]:
Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells - PMC
Description:
A PCR strategy is described for global amplification of DNA from a single eukaryotic cell that enables the comprehensive analysis of the whole genome. By comparative genomic hybridization, not only gross DNA copy number variations, such as monosomic ...
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Keywords {๐}
cells, cell, dna, single, doi, tumor, cgh, pubmed, google, scholar, pcr, marrow, analysis, amplification, bone, cancer, control, genomic, loss, min, sec, genome, amplified, mcf, pmc, line, primary, isolated, performed, hybridization, sequence, syndrome, individual, loh, msei, fragments, profiles, open, tab, normal, showed, chromosomal, chromosome, table, detected, results, fragment, conditions, gene, fig,
Topics {โ๏ธ}
b-phycoerythrin-conjugated goat antibody immunofluorescent cytokeratin-positive cell dinucleotide repeat polymorphism cytokeratin-positive cells originated leica dmxa-rf8 microscope pmc beta search applying matrix/array cgh degenerate oligonucleotide-primed pcr nick-translated dna prepared primer-extension preamplification methods indirect immunofluorescence 1 ฮผl t4-dna-ligase license information pmcid comparative genomic hybridization 50 mm trisโ hcl agarose gel electrophoresis previously published methods dna copy number expected average length publisher site pdf d5s346 locus linked t4 dna ligase degenerate oligonucleotides techniques antigenโantibody complexes disseminated tumor cells single individual genomes expand long template nucleated cell suspension nick-translated dna restriction enzymes recognizing advanced solid metastasis individual cells fails individual diploid cells color interphase fish color interphase-fish single normal cell oleg schmidt-kittler sensys ccd camera ceph-yacs 695h7 sole detectable abnormality bright cytoplasmic staining excellent technical assistance mildred scheel stiftung german federal ministry prog histochem cytochem mcf-7 cell line restriction enzyme digest prospective studies showing individual malignant tumor dna polymerase mixture
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