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Title[redir]:
Micro-RNA Regulation of the Mammalian lin-28 Gene during Neuronal Differentiation of Embryonal Carcinoma Cells - PMC
Description:
Vertebrate genomes each encode hundreds of micro-RNAs (miRNAs), yet for few of these miRNAs is there empirical evidence as to which mRNA(s) they regulate. Here we report the identification of human lin-28 mRNA as a regulatory target of human ...
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Keywords {🔍}
lin, cells, mirb, mire, mrna, gene, reporter, doi, pubmed, google, scholar, mira, rna, human, utr, luciferase, mirna, repression, expression, fig, mirnas, encoding, cell, abundance, mires, protein, plasmid, concentration, elements, genes, copies, pmc, complementary, βgalactosidase, transfected, activity, article, element, elegans, ratio, free, function, effect, translational, retinoic, bearing, microrna, nucleotide, control, dna,
Topics {✒️}
plasmid pbc21/cmv/β-gal noncomplementary 2′-o-methylated oligonucleotide pre-mir-125b stem-loop complementary 2′-o-methylated oligonucleotide pre-mir-125a stem-loop serum-free medium combination hybond-xl nylon membrane plasmid encoding pre-mir-125b nonspecific 2′-o-methyl oligonucleotide 2′ ome anti-mir-125b double-stranded rna directs poly-d-lysine pmc beta search complementary 2′-o-methyl oligonucleotide large stem-loop structure pre-mirna stem-loop polyclonal anti-lin-28 antibodies mirna variant mir-125b-u1a 70-nt stem-loop intermediate agarose-coated petri dishes mir-125b levels rise plasmid encoding β-galactosidase mir-125b-specific probe mir-125b represses lin-28 mir-125a-specific probe accelerated mrna degradation sirna-induced rna silencing gene encoding mir-125b cold spring harbor rna-interference enzyme dicer induce sequence-specific silencing luciferase-sv40 reporter gene naturally produce mir-125b gene encoding mir-125a plasmid encoding mir-125b pre-mir-125b segment α-minimal essential medium mammalian micro-rna homologous human microrna-mrna interactions mirna-related transcript whatsoever mir-125a increases significantly stem-loop structure 2′-o-methyl oligonucleotide mir-125b gene versus putative rna-binding protein mir-125b gene segment retinoic acid-treated cells plasmids encoding mir-125a consecutive base pairs double-stranded rna
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