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Title:
Bystander IFN-γ activity promotes widespread and sustained cytokine signaling altering the tumor microenvironment | Nature Cancer
Description:
The cytokine interferon (IFN)-γ produced by tumor-reactive T cells is a key effector molecule with pleiotropic effects during anti-tumor immune responses. Although IFN-γ production is targeted at the immunologic synapse, its spatiotemporal activity within the tumor remains elusive. In the present study, we report that, although IFN-γ secretion requires local antigen recognition, IFN-γ diffuses extensively to alter the tumor microenvironment in distant areas. Using intravital imaging and a reporter for STAT1 translocation, we provide evidence that T cells mediate sustained IFN-γ signaling in remote tumor cells. Furthermore, tumor phenotypic alterations required several hours of exposure to IFN-γ, a feature that disfavored local IFN-γ activity over diffusion and bystander activity. Finally, single-cell RNA-sequencing data from melanoma patients also suggested bystander IFN-γ activity in human tumors. Thus, tumor-reactive T cells act collectively to create large cytokine fields that profoundly modify the tumor microenvironment. Two papers by Schumacher and colleagues and Bousso and colleagues show that sensing of IFN-γ by a small number of tumor cells leads to propagation of IFN-γ sensing and response across a larger fraction of the tumor.
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nature portfolio journals permissions reprints nature portfolio privacy policy european research council tailed mann-whitney u-test single-cell rna-sequencing data advertising social media tailed unpaired t-test research pre-clinical development content carpet-bombing tumors showing ifn-γ-deficient ot nature+ nature 494 nature 569 nature 545 nature aminomethyl-4-methoxymethyl-5-hydroxy-6-methylpyridine bristol-myers squibb foundation tumor development ifn-γ-mediated inhibition antigen-expressing cellular patches ifn-γ diffuses extensively anti-ifn-γ mab putative ifn-γ concentration dimensional intact-tissue sequencing high-incidence spontaneous lymphoma cell-derived ifn-γ ebv-positive burkitt lymphoma ifn-gamma pathway genes anti-tumor immune responses tunable diffusion–consumption mechanism antigen-positive tumor cells intratumoral ifn-γ concentration ifn-γ-deficient ot single-cell transcriptional states integrative single-cell analysis mu-myc transgenic mouse permissions springerlink instant access rag2-/-γc-/- recipient mice recipient rag2-/-γc-/- mice recipient rag2−/−γc−/− mice ova-expressing eµ-myc eµ-myc cells expressing long-lived inflammation histograms showing h2-kb pd-1 immunoinhibitory receptor
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- Interferon-γ: teammate or opponent in the tumour microenvironment?
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name:Dynamics of Immune Responses Unit, Equipe Labellisée Ligue Contre le Cancer, Institut Pasteur, INSERM U1223, Paris, France
name:University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France
name:Dynamics of Immune Responses Unit, Equipe Labellisée Ligue Contre le Cancer, Institut Pasteur, INSERM U1223, Paris, France
name:Dynamics of Immune Responses Unit, Equipe Labellisée Ligue Contre le Cancer, Institut Pasteur, INSERM U1223, Paris, France
name:Department of Immunology, Weizmann Institute of Science, Rehovot, Israel
name:Dynamics of Immune Responses Unit, Equipe Labellisée Ligue Contre le Cancer, Institut Pasteur, INSERM U1223, Paris, France
name:Dynamics of Immune Responses Unit, Equipe Labellisée Ligue Contre le Cancer, Institut Pasteur, INSERM U1223, Paris, France
name:Systems Biology Group, Center for Bioinformatics, Biostatistics, and Integrative Biology and USR 3756, Institut Pasteur CNRS, Paris, France
name:Dynamics of Immune Responses Unit, Equipe Labellisée Ligue Contre le Cancer, Institut Pasteur, INSERM U1223, Paris, France
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