Here's how NATURE.COM makes money* and how much!

*Please read our disclaimer before using our estimates.
Loading...

NATURE . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Nature.com Make Money
  6. How Much Does Nature.com Make
  7. Keywords
  8. Topics
  9. Schema
  10. Social Networks
  11. External Links
  12. Analytics And Tracking
  13. Libraries
  14. Hosting Providers
  15. CDN Services

We are analyzing https://www.nature.com/articles/s41598-018-21998-3.

Title:
ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages | Scientific Reports
Description:
The NLRC4 inflammasome recognizes bacterial flagellin and components of the type III secretion apparatus. NLRC4 stimulation leads to caspase-1 activation followed by a rapid lytic cell death known as pyroptosis. NLRC4 is linked to pathogen-free auto-inflammatory diseases, suggesting a role for NLRC4 in sterile inflammation. Here, we show that NLRC4 activates an alternative cell death program morphologically similar to apoptosis in caspase-1-deficient BMDMs. By performing an unbiased genome-wide CRISPR/Cas9 screen with subsequent validation studies in gene-targeted mice, we highlight a critical role for caspase-8 and ASC adaptor in an alternative apoptotic pathway downstream of NLRC4. Furthermore, caspase-1 catalytically dead knock-in (Casp1 C284A KI) BMDMs genetically segregate pyroptosis and apoptosis, and confirm that caspase-1 does not functionally compete with ASC for NLRC4 interactions. We show that NLRC4/caspase-8-mediated apoptotic cells eventually undergo plasma cell membrane damage in vitro, suggesting that this pathway can lead to secondary necrosis. Unexpectedly, we found that DFNA5/GSDME, a member of the pore-forming gasdermin family, is dispensable for the secondary necrosis that follows NLRC4-mediated apoptosis in macrophages. Together, our data confirm the existence of an alternative caspase-8 activation pathway diverging from the NLRC4 inflammasome in primary macrophages.
Website Age:
30 years and 10 months (reg. 1994-08-11).

Matching Content Categories {📚}

  • Telecommunications
  • Science
  • Education

Content Management System {📝}

What CMS is nature.com built with?

Custom-built

No common CMS systems were detected on Nature.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of nature.com audience?

🌆 Monumental Traffic: 20M - 50M visitors per month


Based on our best estimate, this website will receive around 42,554,915 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Nature.com Make Money? {💸}


Display Ads {🎯}


The website utilizes display ads within its content to generate revenue. Check the next section for further revenue estimates.

Ads are managed by yourbow.com. Particular relationships are as follows:

Direct Advertisers (10)
google.com, pmc.com, doceree.com, yourbow.com, audienciad.com, onlinemediasolutions.com, advibe.media, aps.amazon.com, getmediamx.com, onomagic.com

Reseller Advertisers (38)
conversantmedia.com, rubiconproject.com, pubmatic.com, appnexus.com, openx.com, smartadserver.com, lijit.com, sharethrough.com, video.unrulymedia.com, google.com, yahoo.com, triplelift.com, onetag.com, sonobi.com, contextweb.com, 33across.com, indexexchange.com, media.net, themediagrid.com, adform.com, richaudience.com, sovrn.com, improvedigital.com, freewheel.tv, smaato.com, yieldmo.com, amxrtb.com, adyoulike.com, adpone.com, criteo.com, smilewanted.com, 152media.info, e-planning.net, smartyads.com, loopme.com, opera.com, mediafuse.com, betweendigital.com

How Much Does Nature.com Make? {💰}


Display Ads {🎯}

$536,300 per month
Estimations show Nature.com's display ad online revenue falls between $357,503 and $983,134 per month.

Keywords {🔍}

caspase, cell, pubmed, death, article, nlrc, cells, bmdms, google, scholar, cas, asc, flagellin, activation, fig, caspcasp, nature, inflammasome, apoptosis, pyroptosis, dfna, macrophages, apoptotic, pathway, prestimulation, central, data, sgrna, ads, release, mice, membrane, secondary, alternative, necrosis, nlrcmediated, gsdmd, caspaseindependent, electroporation, caspasedependent, signaling, yoyo, httpsdoiorgnature, casp, plasma, conditions, pamcsk, ldh, absence, response,

Topics {✒️}

nature portfolio genome-wide crispr/cas9 screens privacy policy genome-wide crispr/cas9 screen genome-wide crispr-cas9 screen triple-deficient casp1−/−casp11−/−asc−/− bmdms advertising unbiased crispr/cas9 screen undergo asc/caspase-8-mediated apoptosis naip5/nlrc4-mediated cell death nlrc4/caspase-8-mediated cell death nlrc4-mas/scan4 patients develop academic libraries 48 tlr/nf-κb activation45 promote nf-κb signaling nlrc4-mediated caspase-1-dependent pyroptosis pathogen-free auto-inflammatory diseases fas/cd95-induced chemokines generated casp1−/−casp11−/−casp8−/−rip3k−/− mice stat-signaling activators ifn-α pathogen-induced cell death undergo caspase-8-mediated apoptosis caspase-8-dependent apoptotic pathway conserved carboxy-terminal domain casp1−/−casp11−/− cas9+ imac reprints caspase-1-independent cell death nlrc4-mediated cell death distinct dfna5-independent program caspase-8-mediated apoptosis pathway flagellin-induced cell death transcriptional anti-apoptotic program39 casp1−/−casp11−/− imacs proceeded interrogated casp1−/−casp11−/− imacs pore-forming gasdermin superfamily block caspase-8-dependent death caspase-1/gsdmd-dependent pyroptosis17 log2 fold-change greater nanoparticle-mediated cell pyroptosis nf-κb signaling tlr signaling-free conditions nf-kappab signals induce nlrc4/caspase-8-mediated apoptosis type-iii secretion system dfna5-independent secondary necrosis pore-forming gasdermin family nf-κb activators multi-protein inflammasome complexes caspase-3-induced secondary necrosis nlrc4-mas/scan4 focus

Schema {🗺️}

WebPage:
      mainEntity:
         headline:ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages
         description:The NLRC4 inflammasome recognizes bacterial flagellin and components of the type III secretion apparatus. NLRC4 stimulation leads to caspase-1 activation followed by a rapid lytic cell death known as pyroptosis. NLRC4 is linked to pathogen-free auto-inflammatory diseases, suggesting a role for NLRC4 in sterile inflammation. Here, we show that NLRC4 activates an alternative cell death program morphologically similar to apoptosis in caspase-1-deficient BMDMs. By performing an unbiased genome-wide CRISPR/Cas9 screen with subsequent validation studies in gene-targeted mice, we highlight a critical role for caspase-8 and ASC adaptor in an alternative apoptotic pathway downstream of NLRC4. Furthermore, caspase-1 catalytically dead knock-in (Casp1 C284A KI) BMDMs genetically segregate pyroptosis and apoptosis, and confirm that caspase-1 does not functionally compete with ASC for NLRC4 interactions. We show that NLRC4/caspase-8-mediated apoptotic cells eventually undergo plasma cell membrane damage in vitro, suggesting that this pathway can lead to secondary necrosis. Unexpectedly, we found that DFNA5/GSDME, a member of the pore-forming gasdermin family, is dispensable for the secondary necrosis that follows NLRC4-mediated apoptosis in macrophages. Together, our data confirm the existence of an alternative caspase-8 activation pathway diverging from the NLRC4 inflammasome in primary macrophages.
         datePublished:2018-02-28T00:00:00Z
         dateModified:2018-02-28T00:00:00Z
         pageStart:1
         pageEnd:12
         license:http://creativecommons.org/licenses/by/4.0/
         sameAs:https://doi.org/10.1038/s41598-018-21998-3
         keywords:
            Apoptosis
            Cell death and immune response
            Science
            Humanities and Social Sciences
            multidisciplinary
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig1_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig2_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig3_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig4_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig5_HTML.jpg
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig6_HTML.jpg
         isPartOf:
            name:Scientific Reports
            issn:
               2045-2322
            volumeNumber:8
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Nature Publishing Group UK
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Bettina L. Lee
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kathleen M. Mirrashidi
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Irma B. Stowe
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Sarah K. Kummerfeld
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Colin Watanabe
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Benjamin Haley
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Trinna L. Cuellar
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Michael Reichelt
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Pathology, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Nobuhiko Kayagaki
               affiliation:
                     name:Genentech Inc., South San Francisco
                     address:
                        name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages
      description:The NLRC4 inflammasome recognizes bacterial flagellin and components of the type III secretion apparatus. NLRC4 stimulation leads to caspase-1 activation followed by a rapid lytic cell death known as pyroptosis. NLRC4 is linked to pathogen-free auto-inflammatory diseases, suggesting a role for NLRC4 in sterile inflammation. Here, we show that NLRC4 activates an alternative cell death program morphologically similar to apoptosis in caspase-1-deficient BMDMs. By performing an unbiased genome-wide CRISPR/Cas9 screen with subsequent validation studies in gene-targeted mice, we highlight a critical role for caspase-8 and ASC adaptor in an alternative apoptotic pathway downstream of NLRC4. Furthermore, caspase-1 catalytically dead knock-in (Casp1 C284A KI) BMDMs genetically segregate pyroptosis and apoptosis, and confirm that caspase-1 does not functionally compete with ASC for NLRC4 interactions. We show that NLRC4/caspase-8-mediated apoptotic cells eventually undergo plasma cell membrane damage in vitro, suggesting that this pathway can lead to secondary necrosis. Unexpectedly, we found that DFNA5/GSDME, a member of the pore-forming gasdermin family, is dispensable for the secondary necrosis that follows NLRC4-mediated apoptosis in macrophages. Together, our data confirm the existence of an alternative caspase-8 activation pathway diverging from the NLRC4 inflammasome in primary macrophages.
      datePublished:2018-02-28T00:00:00Z
      dateModified:2018-02-28T00:00:00Z
      pageStart:1
      pageEnd:12
      license:http://creativecommons.org/licenses/by/4.0/
      sameAs:https://doi.org/10.1038/s41598-018-21998-3
      keywords:
         Apoptosis
         Cell death and immune response
         Science
         Humanities and Social Sciences
         multidisciplinary
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig1_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig2_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig3_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig4_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig5_HTML.jpg
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41598-018-21998-3/MediaObjects/41598_2018_21998_Fig6_HTML.jpg
      isPartOf:
         name:Scientific Reports
         issn:
            2045-2322
         volumeNumber:8
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Nature Publishing Group UK
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Bettina L. Lee
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kathleen M. Mirrashidi
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Irma B. Stowe
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Sarah K. Kummerfeld
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Colin Watanabe
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Benjamin Haley
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Trinna L. Cuellar
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Michael Reichelt
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Pathology, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Nobuhiko Kayagaki
            affiliation:
                  name:Genentech Inc., South San Francisco
                  address:
                     name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Scientific Reports
      issn:
         2045-2322
      volumeNumber:8
Organization:
      name:Nature Publishing Group UK
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Pathology, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
      name:Genentech Inc., South San Francisco
      address:
         name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Bettina L. Lee
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Kathleen M. Mirrashidi
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Irma B. Stowe
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Sarah K. Kummerfeld
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Colin Watanabe
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Benjamin Haley
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Trinna L. Cuellar
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Michael Reichelt
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Pathology, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      name:Nobuhiko Kayagaki
      affiliation:
            name:Genentech Inc., South San Francisco
            address:
               name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
      name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
      name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA
      name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
      name:Department of Bioinformatics, Genentech Inc., South San Francisco, California, USA
      name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
      name:Department of Molecular Biology, Genentech Inc., South San Francisco, California, USA
      name:Department of Pathology, Genentech Inc., South San Francisco, California, USA
      name:Department of Physiological Chemistry, Genentech Inc., South San Francisco, California, USA

External Links {🔗}(285)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Prism.js
  • Zoom.js

Emails and Hosting {✉️}

Mail Servers:

  • mxa-002c5801.gslb.pphosted.com
  • mxb-002c5801.gslb.pphosted.com

Name Servers:

  • pdns1.ultradns.net
  • pdns2.ultradns.net
  • pdns3.ultradns.org
  • pdns4.ultradns.org
  • pdns5.ultradns.info
  • pdns6.ultradns.co.uk

CDN Services {📦}

  • Crossref

4.57s.