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We are analyzing https://www.nature.com/articles/s41587-024-02470-2.

Title:
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys | Nature Biotechnology
Description:
Transplantation of ex vivo engineered hematopoietic stem cells (HSCs) can lead to robust clinical responses but carries risks of adverse events from bone marrow mobilization, chemotherapy conditioning and other factors. HSCs have been modified in vivo using lipid nanoparticles (LNPs) decorated with targeting moieties, which increases manufacturing complexity. Here we screen 105 LNPs without targeting ligands for effective homing to the bone marrow in mouse. We report an LNP named LNP67 that delivers mRNA to murine HSCs in vivo, primary human HSCs ex vivo and CD34+ cells in rhesus monkeys (Macaca mulatta) in vivo at doses of 0.25 and 0.4 mg kg−1. Without mobilization and conditioning, LNP67 can mediate delivery of mRNA to HSCs and their progenitor cells (HSPCs), as well as to the liver in rhesus monkeys, without serum cytokine activation. These data support the hypothesis that in vivo delivery to HSCs and HSPCs in nonhuman primates is feasible without targeting ligands. A lipid nanoparticle is used to deliver mRNA to hematopoietic stem cells in macaques.
Website Age:
30 years and 10 months (reg. 1994-08-11).

Matching Content Categories {📚}

  • Education
  • Science
  • Telecommunications

Content Management System {📝}

What CMS is nature.com built with?

Custom-built

No common CMS systems were detected on Nature.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of nature.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


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How Does Nature.com Make Money? {💸}


Display Ads {🎯}


The website utilizes display ads within its content to generate revenue. Check the next section for further revenue estimates.

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Direct Advertisers (10)
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Reseller Advertisers (38)
conversantmedia.com, rubiconproject.com, pubmatic.com, appnexus.com, openx.com, smartadserver.com, lijit.com, sharethrough.com, video.unrulymedia.com, google.com, yahoo.com, triplelift.com, onetag.com, sonobi.com, contextweb.com, 33across.com, indexexchange.com, media.net, themediagrid.com, adform.com, richaudience.com, sovrn.com, improvedigital.com, freewheel.tv, smaato.com, yieldmo.com, amxrtb.com, adyoulike.com, adpone.com, criteo.com, smilewanted.com, 152media.info, e-planning.net, smartyads.com, loopme.com, opera.com, mediafuse.com, betweendigital.com

How Much Does Nature.com Make? {💰}


Display Ads {🎯}

$63,100 per month
Our estimates place Nature.com's monthly online earnings from display ads at $42,042 to $115,616.

Keywords {🔍}

pubmed, article, google, scholar, cas, central, nature, vivo, cell, delivery, lipid, mrna, nanoparticles, cells, data, stem, med, nat, hematopoietic, editing, access, engl, information, gene, usa, content, rhesus, tarantal, targeting, ther, research, university, cookies, hscs, review, mol, singlecell, jed, davis, privacy, biotechnology, monkeys, kim, lnp, nonhuman, genome, national, center, srr, crisprcas,

Topics {✒️}

gov/vaccines-blood-biologics/biologics-guidances/cellular-gene-therapy-guidances /news-releases/news-release-details/verve-therapeutics-doses nature portfolio journals nature-inspired platform nanotechnology nature portfolio privacy policy crispr–cas gene editing advertising fourier light-field microscopy β0/β0 genotype β-thalassemia translational research crispr–cas9 gene editing social media bone-marrow-homing lipid nanoparticles visualizing single-cell data multimodal single-cell data reprints gov/datasets/genome/gcf_000001635 anti-peg antibodies inhibit lung-targeting lipid nanoparticles vivo gene editing tissue-specific mrna delivery nature+ nature 592 nature mrna-1273 sars-cov-2 vaccine publishing agreement vivo genome editing messenger rna delivery cryo-tem data gov/editing targeted lipid nanoparticles lipid nanoparticle formulations permissions verve therapeutics https cryo-tem imaging springerlink instant access genome editing lipid nanoparticles enabled accepted manuscript version gene therapy human-investigational-vivo-base broadly neutralizing antibodies vivo rna delivery hematopoietic stem cells reveal endothelial niches sirna lipid nanoparticles selective organ targeting single-cell analysis vivo mrna delivery

Schema {🗺️}

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