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Keywords {🔍}

mrna, rna, casa, cells, sarscov, guides, infection, fig, article, supplementary, influenza, cas, ntcr, viral, guide, google, scholar, data, virus, delivery, performed, mice, knockdown, nature, hamsters, crrna, infected, min, hpi, multiple, cell, expression, strains, experiments, transfected, moi, approach, anova, lungs, scientific, animal, casanls, lung, qpcr, targeted, awsn, animals, gene, sequence, ratio,

Topics {✒️}

nature portfolio permissions reprints privacy policy unconventional molecular tools advertising hepa-filtered cage/rack system initial direct-repeat stem-loop physiological research laboratory regional mortality sars-cov-2 research broad-spectrum crispr-mediated inhibition middle 3d-printed nose cones stable stem-loop structure regn-cov2 antibodies prevent nature 550 nature 459 nature 538 nature 583 nature cy3b nhs-ester dye anti-tumor responses sebastian crispr-dcas13 system lekha glacial acetic acid prokaryote nucleotide-binding domain16 chloroform-based phase separation research design serum-free media sybr green-based commercially rna-targeting type vi dry ice-cooled isopentane rabbit anti-v5 tag sars-cov-2 genomes showing 10-μm-thick tissue sections taqman primer/probe assays n1-methyl-pseudouridine modified current sars-cov-2 pandemic10 nano-glo substrate solution rt–qpcr technical triplicates sars-cov-2-infected syrian hamsters current drug development sars-cov-2 copy number mouse monoclonal anti-influenza larger-diameter animal restraints mitigate sars-cov-2 infections permissions human-infective rna viruses cas13a-mediated immune responses anchored vhh-encoding mrna excitation 485 ± 20 nm/emission 528 ± 20 nm

Questions {❓}

• Finally, we answered a critical question regarding Cas13: can it be used in vivo against respiratory viruses?
• We then addressed the question: can this approach be applied directly to newly emerging pathogens such as SARS-CoV-2?
• Why polymerase genes?
• Why synthetic mRNA?

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         name:Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine University of Georgia, Athens, USA
         type:PostalAddress
      name:College of Veterinary Medicine University of Georgia
      address:
         name:Department of Infectious Diseases, College of Veterinary Medicine University of Georgia, Athens, USA
         type:PostalAddress
      name:Georgia Institute of Technology and Emory University
      address:
         name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
         type:PostalAddress
      name:Georgia Institute of Technology and Emory University
      address:
         name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
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      name:Emmeline L. Blanchard
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
               type:PostalAddress
            type:Organization
      name:Daryll Vanover
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
               type:PostalAddress
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      name:Swapnil Subhash Bawage
      url:http://orcid.org/0000-0001-9139-5375
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
               type:PostalAddress
            type:Organization
      name:Pooja Munnilal Tiwari
      url:http://orcid.org/0000-0001-8170-3602
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
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            type:Organization
      name:Laura Rotolo
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
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      name:Jared Beyersdorf
      url:http://orcid.org/0000-0002-6997-4175
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
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      name:Hannah E. Peck
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            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
               type:PostalAddress
            type:Organization
      name:Nicholas C. Bruno
      affiliation:
            name:School of Chemistry and Biochemistry, Georgia Institute of Technology
            address:
               name:School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA
               type:PostalAddress
            type:Organization
      name:Robert Hincapie
      url:http://orcid.org/0000-0001-7358-4543
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            address:
               name:School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA
               type:PostalAddress
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      name:Frank Michel
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            address:
               name:Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine University of Georgia, Athens, USA
               type:PostalAddress
            type:Organization
      name:Jackelyn Murray
      affiliation:
            name:College of Veterinary Medicine University of Georgia
            address:
               name:Department of Infectious Diseases, College of Veterinary Medicine University of Georgia, Athens, USA
               type:PostalAddress
            type:Organization
      name:Heena Sadhwani
      affiliation:
            name:Georgia State University
            address:
               name:Department of Biology, Georgia State University, Atlanta, USA
               type:PostalAddress
            type:Organization
      name:Bob Vanderheyden
      affiliation:
            name:Kennesaw State University
            address:
               name:Analytics and Data Science Institute, Kennesaw State University, Kennesaw, USA
               type:PostalAddress
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      name:M. G. Finn
      url:http://orcid.org/0000-0001-8247-3108
      affiliation:
            name:School of Chemistry and Biochemistry, Georgia Institute of Technology
            address:
               name:School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA
               type:PostalAddress
            type:Organization
      name:Margo A. Brinton
      affiliation:
            name:Georgia State University
            address:
               name:Department of Biology, Georgia State University, Atlanta, USA
               type:PostalAddress
            type:Organization
      name:Eric R. Lafontaine
      affiliation:
            name:College of Veterinary Medicine University of Georgia
            address:
               name:Department of Infectious Diseases, College of Veterinary Medicine University of Georgia, Athens, USA
               type:PostalAddress
            type:Organization
      name:Robert J. Hogan
      url:http://orcid.org/0000-0002-0225-830X
      affiliation:
            name:College of Veterinary Medicine University of Georgia
            address:
               name:Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine University of Georgia, Athens, USA
               type:PostalAddress
            type:Organization
            name:College of Veterinary Medicine University of Georgia
            address:
               name:Department of Infectious Diseases, College of Veterinary Medicine University of Georgia, Athens, USA
               type:PostalAddress
            type:Organization
      name:Chiara Zurla
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Philip J. Santangelo
      url:http://orcid.org/0000-0001-7352-0339
      affiliation:
            name:Georgia Institute of Technology and Emory University
            address:
               name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA
      name:School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA
      name:Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine University of Georgia, Athens, USA
      name:Department of Infectious Diseases, College of Veterinary Medicine University of Georgia, Athens, USA
      name:Department of Biology, Georgia State University, Atlanta, USA
      name:Analytics and Data Science Institute, Kennesaw State University, Kennesaw, USA
      name:School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, USA
      name:Department of Biology, Georgia State University, Atlanta, USA
      name:Department of Infectious Diseases, College of Veterinary Medicine University of Georgia, Athens, USA
      name:Department of Veterinary Biosciences and Diagnostic Imaging, College of Veterinary Medicine University of Georgia, Athens, USA
      name:Department of Infectious Diseases, College of Veterinary Medicine University of Georgia, Athens, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA
      name:Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA

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