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Title:
Antibiotic-loaded nanoparticles targeted to the site of infection enhance antibacterial efficacy | Nature Biomedical Engineering
Description:
Bacterial resistance to antibiotics has made it necessary to resort to using antibacterial drugs that have considerable toxicities. Here, we show that conjugation of vancomycin-loaded nanoparticles with the cyclic 9-amino-acid peptide CARGGLKSC (CARG), identified via phage display on Staphylococcus aureus (S. aureus) bacteria and through in vivo screening in mice with S. aureus-induced lung infections, increases the antibacterial activity of the nanoparticles in S. aureus-infected tissues and reduces the systemic dose needed, minimizing side effects. CARG binds specifically to S. aureus bacteria but not Pseudomonas bacteria in vitro, selectively accumulates in S. aureus-infected lungs and skin of mice but not in non-infected tissue and Pseudomonas-infected tissue, and significantly enhances the accumulation of intravenously injected vancomycin-loaded porous silicon nanoparticles bearing CARG in S. aureus-infected mouse lung tissue. The targeted nanoparticles more effectively suppress staphylococcal infections in vivo relative to equivalent doses of untargeted vancomycin nanoparticles or of free vancomycin. The therapeutic delivery of antibiotic-carrying nanoparticles bearing peptides targeting infected tissues may help combat difficult-to-treat infections. Nanoparticles carrying an antibiotic and conjugated with a peptide identified via phage display that binds specifically to Staphylococcus aureus effectively suppress staphylococcal infections in vivo.
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nature portfolio journals nature portfolio permissions reprints interest policies folic-acid-based receptor targeting privacy policy advertising antibiotic-loaded nanoparticles targeted research findings included nature+ nature 527 nature scientific founder methicillin-resistant staphylococcus aureus killing multidrug-resistant bacteria iron-regulating sigma factors tumor-penetrating peptide enhances peptide-conjugated quantum dots ly6g-specific monoclonal antibody porous silicon nanoparticles tumor vasculature-targeted imaging social media business media aureus-induced lung infections author correspondence engineering program antimicrobial resistance aureus-infected tissues overcome bacterial resistance research vancomycin-loaded nanoparticles permissions vancomycin-modified nanoparticles springerlink instant access interest management based tumor-homing peptide policy asan medical center personal data tambet teesalu staphylococcus-binding peptide pseudomonas-binding peptide pseudomonas-infected tissue università di messina published maps mesoporous silicon particles porous silicon development enhance antibacterial efficiency methicillin-resistant
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- Are bloodstream leukocytes Trojan horses for the metastasis of Staphylococcus aureus?
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headline:Antibiotic-loaded nanoparticles targeted to the site of infection enhance antibacterial efficacy
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type:PostalAddress
type:Organization
name:Aman P. Mann
affiliation:
name:Sanford Burnham Prebys Medical Discovery Institute
address:
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
type:PostalAddress
type:Organization
name:Tarmo Mölder
affiliation:
name:University of Tartu
address:
name:Laboratory of Cancer Biology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
type:PostalAddress
type:Organization
name:Tambet Teesalu
affiliation:
name:Sanford Burnham Prebys Medical Discovery Institute
address:
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
type:PostalAddress
type:Organization
name:University of Tartu
address:
name:Laboratory of Cancer Biology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
type:PostalAddress
type:Organization
name:University of California, Santa Barbara
address:
name:Center for Nanomedicine, and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, USA
type:PostalAddress
type:Organization
name:Santina Carnazza
affiliation:
name:Università di Messina
address:
name:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali- ChiBioFarAm, Università di Messina, Messina, Italy
type:PostalAddress
type:Organization
name:Salvatore Guglielmino
affiliation:
name:Università di Messina
address:
name:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali- ChiBioFarAm, Università di Messina, Messina, Italy
type:PostalAddress
type:Organization
name:Michael J. Sailor
url:http://orcid.org/0000-0002-4809-9826
affiliation:
name:University of California, San Diego
address:
name:Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, USA
type:PostalAddress
type:Organization
name:University of California, San Diego
address:
name:Department of Nanoengineering, University of California, San Diego, La Jolla, USA
type:PostalAddress
type:Organization
name:University of California, San Diego
address:
name:Materials Science and Engineering Program, University of California, San Diego, La Jolla, USA
type:PostalAddress
type:Organization
name:Erkki Ruoslahti
affiliation:
name:Sanford Burnham Prebys Medical Discovery Institute
address:
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
type:PostalAddress
type:Organization
name:University of California, Santa Barbara
address:
name:Center for Nanomedicine, and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, USA
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
name:Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, USA
name:Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea
name:Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul, Republic of Korea
name:Department of Nanoengineering, University of California, San Diego, La Jolla, USA
name:Materials Science and Engineering Program, University of California, San Diego, La Jolla, USA
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
name:STEMCELL Technologies Inc., Vancouver, Canada
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
name:Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
name:Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, USA
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
name:Laboratory of Cancer Biology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
name:Laboratory of Cancer Biology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia
name:Center for Nanomedicine, and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, USA
name:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali- ChiBioFarAm, Università di Messina, Messina, Italy
name:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali- ChiBioFarAm, Università di Messina, Messina, Italy
name:Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, USA
name:Department of Nanoengineering, University of California, San Diego, La Jolla, USA
name:Materials Science and Engineering Program, University of California, San Diego, La Jolla, USA
name:Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA
name:Center for Nanomedicine, and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, USA
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