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We are analyzing https://www.nature.com/articles/s41467-022-33831-7.

Title:
In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses | Nature Communications
Description:
Cancer vaccine, which can promote tumor-specific immunostimulation, is one of the most important immunotherapeutic strategies and holds tremendous potential for cancer treatment/prevention. Here, we prepare a series of nanoparticles composed of doxorubicin- and tyrosine kinase inhibitor-loaded and hyaluronic acid-coated dendritic polymers (termed HDDT nanoparticles) and find that the HDDT nanoparticles can convert various cancer cells to micrometer-sized vesicles (1.6−3.2 μm; termed HMVs) with ~100% cell-to-HMV conversion efficiency. We confirm in two tumor-bearing mouse models that the nanoparticles can restrain tumor growth, induce robust immunogenic cell death, and convert the primary tumor into an antigen depot by producing HMVs in situ to serve as personalized vaccines for cancer immunotherapy. Furthermore, the HDDT-healed mice show a strong immune memory effect and the HDDT treatment can realize long-term protection against tumor rechallenge. Collectively, the present work provides a general strategy for the preparation of tumor-associated antigen-containing vesicles and the development of personalized cancer vaccines. Autologous tumor cell vaccines can elicit anti-tumor immune responses. Here, the authors report the design of dendritic polymer-based nanoparticles loaded with doxorubicin and the tyrosine kinase inhibitor apatinib that can induce immunogenic cell death and the in situ production of immuno-stimulatory micrometer-sized vesicles, promoting anti-tumor immune responses in preclinical models.
Website Age:
30 years and 10 months (reg. 1994-08-11).

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Keywords {🔍}

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Topics {✒️}

nature portfolio privacy policy india ink-stained lungs t-helper-17-cell-mediated anti-tumour immunity metal−phenolic network-based nanocomplexes advertising cation-independent mannose-6-phosphate receptor anti-mouse glyceraldehyde-3-phosphate dehydrogenase fundamental research funds anti-mouse mch ii-pe hddt-induced micrometer-sized vesicles anti-mouse mhc ii-pe anti-mouse cd86-pe-cy7 nature 526 nature �hdda + anti-pd-1”-treated tumor tissues annexin v-fitc/propidium iodide research design hdda solutions/suspensions anti-mouse cd8-pe-cy7 reprints anti-mouse cd44-fitc antibody anti-mouse cd11c-pe antibodies uniform micrometer-sized vesicles immunology-guided biomaterial design author information authors anti-mhc ii-pe anti-cd86-pe-cy7 �hdda + anti-pd-1”-healed mice antibodies-stained single-cell suspensions �hdda + anti-pd-1” treatment showed uranyl acetate/lead citrate hif-1 hypoxia-inducible factor-1 dna nanodevice-based vaccine cytokine–cytokine receptor interaction data-dependent acquisition mode cancer cell-derived vesicles hdda-induced systemic inflammation bacteria-derived membrane vesicles reporting summary fu-gen wu elisa kits myeloid-derived suppressor cells enzyme-linked immunosorbent assay real-time proliferation processes hdda-induced cell death cd11c+cd86+mhc ii+ high-performance liquid chromatography anti-cd8-pe-cy7 anti-cd44-fluorescein isothiocyanate

Schema {🗺️}

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      headline:In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses
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      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Xiaodong Zhang
      url:http://orcid.org/0000-0003-4137-3535
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Ge Gao
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Yao-Wen Jiang
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Chengcheng Li
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Xiaokai Chen
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Shun-Yu Wu
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Yi Liu
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      name:Fu-Gen Wu
      url:http://orcid.org/0000-0003-1773-2868
      affiliation:
            name:Southeast University
            address:
               name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China
      name:State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China

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