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We are analyzing https://www.nature.com/articles/s41467-019-10903-9.

Title:
IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation | Nature Communications
Description:
The uptake of apoptotic polymorphonuclear cells (PMN) by macrophages is critical for timely resolution of inflammation. High-burden uptake of apoptotic cells is associated with loss of phagocytosis in resolution phase macrophages. Here, using a transcriptomic analysis of macrophage subsets, we show that non-phagocytic resolution phase macrophages express a distinct IFN-β-related gene signature in mice. We also report elevated levels of IFN-β in peritoneal and broncho-alveolar exudates in mice during the resolution of peritonitis and pneumonia, respectively. Elimination of endogenous IFN-β impairs, whereas treatment with exogenous IFN-β enhances, bacterial clearance, PMN apoptosis, efferocytosis and macrophage reprogramming. STAT3 signalling in response to IFN-β promotes apoptosis of human PMNs. Finally, uptake of apoptotic cells promotes loss of phagocytic capacity in macrophages alongside decreased surface expression of efferocytic receptors in vivo. Collectively, these results identify IFN-β produced by resolution phase macrophages as an effector cytokine in resolving bacterial inflammation. Clearance of apoptotic neutrophils by macrophages is important for the resolution of inflammation. Here, the authors show that interferon-β produced by resolution phase macrophages promotes neutrophil apoptosis and efferocytosis and induces macrophage reprogramming to a pro-resolving phenotype, thereby identifying IFN-β as a multi-pronged pro-resolution cytokine.
Website Age:
30 years and 10 months (reg. 1994-08-11).

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Keywords {🔍}

macrophages, ifnβ, cells, fig, apoptotic, mice, resolution, article, ifnb, pmn, google, scholar, inflammation, peritoneal, apoptosis, cas, expression, nonphagocytic, phase, macrophage, dna, results, cell, neutrophils, data, ppi, efferocytosis, human, experiments, uptake, nature, coli, vivo, recovered, phagocytic, neutrophil, cpg, source, supplementary, culture, percentage, analysis, levels, injected, peritonitis, increased, reduced, proresolving, phagocytosis, reprogramming,

Topics {✒️}

nature portfolio privacy policy lipophilic fluorochrome chloromethyl-x-rosamine advertising macrophage-derived multi-pronged effector nature communications pe-conjugated anti-f4/80 abs apc-conjugated anti-mouse tim4 anti-ifn-β-treated wt mice specific pathogen-free conditions tlr9-mediated ifn-β production ifn-beta-stat3-dependent pathway anti-ifn-β antibody delayed source data file libraries reprints isotype-matched irrelevant ab phagocytic nature rabbit anti-mouse isg-15 pan-caspase inhibitor q-vd clinical research committee research center pro-resolving cytokine tgf-β percp anti-mouse cd45 apc anti-mouse mertk recombinant mouse ifn-β1 isotype-matched rat igg1 cyclin-dependent kinase inhibitors1 exogenous ifn-β enhances intracellular ifn-β protein pe anti-mouse f4/80 pe-conjugated gr1 antibodies atp-binding cassette a1 anti-ifn-β antibody calcium-dependent proapoptotic effect pro-resolving lipid mediators exogenous ifn-β acts ifn-β directly promotes mouse recombinant ifn-β nature nature 510 nature 484 nature 390 nature 477 nature 416 lipopolysaccharide-induced endotoxin shock nf-kappab signaling pathways endogenous ifn-β impairs research design blocking ifn-β abrogates

Schema {🗺️}

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      headline:IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation
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      name:Senthil Kumaran Satyanarayanan
      url:http://orcid.org/0000-0002-0513-4943
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Driss El Kebir
      affiliation:
            name:University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital
            address:
               name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
               type:PostalAddress
            type:Organization
      name:Soaad Soboh
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Sergei Butenko
      url:http://orcid.org/0000-0001-9759-9897
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Meriem Sekheri
      affiliation:
            name:University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital
            address:
               name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
               type:PostalAddress
            type:Organization
      name:Janan Saadi
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Neta Peled
      url:http://orcid.org/0000-0001-9128-8422
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Simaan Assi
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Amira Othman
      affiliation:
            name:University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital
            address:
               name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
               type:PostalAddress
            type:Organization
      name:Sagie Schif-Zuck
      url:http://orcid.org/0000-0001-5415-9488
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Yonatan Feuermann
      affiliation:
            name:ResCure Pharma
            address:
               name:ResCure Pharma, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Dalit Barkan
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:Noa Sher
      affiliation:
            name:University of Haifa
            address:
               name:Tauber Bioinformatics Center, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      name:János G. Filep
      url:http://orcid.org/0000-0001-5022-3534
      affiliation:
            name:University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital
            address:
               name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Amiram Ariel
      affiliation:
            name:University of Haifa
            address:
               name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:ResCure Pharma, Haifa, Israel
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel
      name:Tauber Bioinformatics Center, University of Haifa, Haifa, Israel
      name:Department of Pathology and Cell Biology, University of Montreal, and Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada
      name:Department of Biology and Human Biology, University of Haifa, Haifa, Israel

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