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recseq, cre, dna, specificity, article, fig, google, scholar, cas, loxp, recombinase, positions, brec, tre, recombinases, substrate, supplementary, substrates, interactions, position, enrichment, halfsite, recombination, ssrs, cells, activity, sequence, data, wildtype, nature, sequences, base, residues, preference, offtarget, showed, genome, proteindna, bxb, target, library, acids, relative, site, evolved, variants, ssr, halfsites, values, res,

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search parameters a14c13nt11nnt8a7t6t5nnnnnnnnt5′a6′t7′nnc10′a11′a12′ c11ng9nt7t6a5nnnnnnnnt5′a6′a7′nnc10′nt12′g13′ nature portfolio privacy policy slide-a-lyzer dialysis cassette 1 mm isopropyl-β-d-1-thiogalactopyranoside advertising nature communications poly-d-lysine-coated plates pcalnl-egfp-bsai acceptor vector pbt100-neomycin-terminator donor vector tyrosine-based site-specific recombinases high-throughput sequencing-based method reprints author information authors site-specific recombination synapse zymo research corporation modified pcalnl-egfp-esp3i vector half-site libraries based ptet-mrfp cassette flanked endotoxin-removal plasmid-purification system half-site log-enrichment values nature 389 nature cationic lipid-mediated delivery site-specific recombination systems pcalnl-gfp subcloning vector full log-enrichment profile phage-encoded serine integrases yeast endonuclease i-scei bac/pac recombineering techniques post-recombination library versus t-sne visualization relates long-range specificity-determining role source data file efficient site-specific integration donor pbt100-neomycin-terminator single-stranded bridging oligonucleotides model-guided engineering site-specific genomic integration pcalnl-egfp-esp3i vector19 high-throughput dna sequencing multi-dimensional similarity analysis n-terminal tev-cleavable pcalnl-egfp-esp3i vector full rec-seq profile high-resolution conditional mutagenesis post-recombinase-treated dataset r1–r4 half-sites flanking site-specific dna recombinases

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