We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-260-5_18.

Title:
mRNA Electroporation as a Tool for Immunomonitoring | SpringerLink
Description:
Monitoring the immune response is an essential aspect of numerous clinical vaccination trials in order to evaluate the efficacy. In these clinical vaccination trials, peripheral blood mononuclear cells (PBMC) are isolated at different time points from patient blood...
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Questions {❓}

Britten CM, Janetzki S, van der Burg SH, Gouttefangeas C, Hoos A (2008) Toward the harmonization of immune monitoring in clinical trials: quo vadis?
Kiecker F, Streitz M, Ay B, Cherepnev G, Volk HD, Volkmer-Engert R, Kern F (2004) Analysis of antigen-specific T-cell responses with synthetic peptides—what kind of peptide for which purpose?

Schema {🗺️}

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      headline:mRNA Electroporation as a Tool for Immunomonitoring
      pageEnd:303
      pageStart:293
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-260-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Synthetic Messenger RNA and Cell Metabolism Modulation
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            978-1-62703-260-5
            978-1-62703-259-9
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         name:Humana Press
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      author:
            name:Nathalie Cools
            affiliation:
                  name:University of Antwerp
                  address:
                     name:Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine & Health Sciences, University of Antwerp, Wilrijk, Belgium
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            email:[email protected]
            type:Person
            name:Kirsten Van Camp
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                     type:PostalAddress
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            name:Viggo Van Tendeloo
            affiliation:
                  name:University of Antwerp
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                     type:PostalAddress
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            name:Zwi Berneman
            affiliation:
                  name:University of Antwerp
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                     name:Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine & Health Sciences, University of Antwerp, Wilrijk, Belgium
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      keywords:mRNA, Electroporation, PBMC, Cryopreservation, Immunomonitoring
      description:Monitoring the immune response is an essential aspect of numerous clinical vaccination trials in order to evaluate the efficacy. In these clinical vaccination trials, peripheral blood mononuclear cells (PBMC) are isolated at different time points from patient blood samples and subsequently cryopreserved to allow batch analysis at a later time point. Here, we present a newly developed short-time assay which allows direct ex vivo analysis of multi-epitope antigen-specific immune responses using mRNA electroporation of cryopreserved PBMC. This novel method is a rapid and elegant tool and will be convenient for monitoring the cellular immune status of patients in clinical vaccination settings.
      datePublished:2013
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      email:[email protected]
      name:Kirsten Van Camp
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      name:Viggo Van Tendeloo
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      name:Zwi Berneman
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