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We began analyzing https://www.nature.com/articles/2403463, but it redirected us to https://www.nature.com/articles/2403463. The analysis below is for the second page.

Title[redir]:
RNA-based gene transfer for adult stem cells and T cells | Leukemia
Description:
Electroporation of mRNA has become an established method for gene transfer into dendritic cells for immunotherapeutic purposes. However, many more cell types and applications might benefit from an efficient mRNA-based gene transfer method. In this study, we investigated the potential of mRNA-based gene transfer to induce short-term transgene expression in adult stem cells and activated T cells, based on electroporation with mRNA encoding the enhanced green fluorescent protein. The results show efficient transgene expression in CD34-positive hematopoietic progenitor cells (35%), in in vitro cultured mesenchymal cells (90%) and in PHA-stimulated T cells (50%). Next to presentation of gene transfer results, potential applications of mRNA-based gene transfer in stem cells and T cells are discussed.

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Keywords {🔍}

article, cells, google, scholar, cas, gene, nature, van, human, leukemia, transfer, access, stem, ponsaerts, mrna, expression, therapy, tendeloo, content, dendritic, cookies, electroporation, cell, hematopoietic, immunol, bone, privacy, methods, lenjou, nijs, berneman, applications, efficient, progenitor, primary, lymphocytes, open, blood, research, data, adult, smits, mrnabased, transgene, vitro, mesenchymal, cancer, grant, scientific, author,

Topics {✒️}

nature portfolio verzekeringen-financed cancer research permissions reprints privacy policy cd8-mediated t-cell responses high-level transgene expression nature 2002 nature advertising electroporation-mediated gene delivery social media tools rna-based gene transfer fiber-mutant adenoviral vector mrna-based gene transfer infectious epstein–barr virus autologous epstein–barr virus rna-loaded dendritic cells rna-transfected dendritic cells gene-based cancer vaccines van tendeloo vf scientific committee personal data springerlink instant access author correspondence human hematopoietic cd34 mesenchymal stem cell data protection gene inhibits proliferation permissions mesenchymal stem cells gene transfer results human dendritic cells human hematopoietic cells adenovirus vector successful gene therapy human haematopoietic stem immunol methods 2002 immunol methods 2003 privacy microfluidic transfection mrna-based electrotransfection cell cycle kinetics adult stem cells human primary lymphocytes primary carcinoembryonic antigen progenitor cells telomerase catalytic subunit explore content subscription content

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