We are analyzing https://link.springer.com/protocol/10.1007/978-1-0716-1060-2_10.

Title:
In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function | SpringerLink
Description:
Myeloid-derived suppressor cells (MDSC) appear at relatively low frequencies in diseased organs such as tumors or infection sites, but accumulate systemically in the spleen. So far MDSC have been reported in humans and experimental animals such as mice, rats, and...
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Keywords {🔍}

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Topics {✒️}

gm-csf monocyte-derived cells myeloid-derived suppressor cells myeloid-derived suppressor activity month download article/chapter akt/mtor license monocytes monocytic antigen-presenting cells myeloid dendritic cells murine dendritic cells tumor-induced tolerance ifn-gr/irf-1 privacy choices/manage cookies dendritic cell family device instant download cell-suppressive activity single-cell transcriptomics author information authors gm-csf signals bone marrow suppress nitric oxide production macrophage marker expression mouse bone marrow tumor-bearing mice eliana ribechini & manfred springer nature jordán garrote a de bruijn mf european economic area advanced culture method immune suppression depend disabling antitumor cytotoxicity monocytic lineages maintained intrinsic death pathways fetal calf serum editor information editors friedrich-loeffler-institut greifswald-insel riems conditions privacy policy van den bossche van den bergh generating large quantities journal finder publish van ginderachter ja protocol eckert accepting optional cookies gabrilovich di define developmental stages main content log fung jj prolong allograft survival protocol usd 49

Schema {🗺️}

ScholarlyArticle:
      headline:In Vitro Generation of Murine Myeloid-Derived Suppressor Cells, Analysis of Markers, Developmental Commitment, and Function
      pageEnd:114
      pageStart:99
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-0716-1060-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Myeloid-Derived Suppressor Cells
         isbn:
            978-1-0716-1060-2
            978-1-0716-1059-6
         type:Book
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Ina Eckert
            affiliation:
                  name:University of Würzburg
                  address:
                     name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Eliana Ribechini
            affiliation:
                  name:University of Würzburg
                  address:
                     name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Manfred B. Lutz
            affiliation:
                  name:University of Würzburg
                  address:
                     name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Myeloid-derived suppressor cells, In vitro generation , Differentiation , Activation , GM-CSF
      description:Myeloid-derived suppressor cells (MDSC) appear at relatively low frequencies in diseased organs such as tumors or infection sites, but accumulate systemically in the spleen. So far MDSC have been reported in humans and experimental animals such as mice, rats, and nonhuman primates. Therefore, methods to generate MDSC in large amounts in vitro can serve as an additional tool to study their biology. Here, we describe in detail the generation of murine MDSC with GM-CSF from bone marrow (BM). Both subsets of granulocytic (G-MDSC) and monocytic MDSC (M-MDSC) are generated by this cytokine. We provide panels of phenotypic markers to distinguish them from non-suppressive cells and define developmental stages of monocytes developing into M-MDSC by two subsequent steps in vitro.
      datePublished:2021
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Book:
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      isbn:
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         978-1-0716-1059-6
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      name:Springer US
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Würzburg
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      name:University of Würzburg
      address:
         name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
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      name:University of Würzburg
      address:
         name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
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Person:
      name:Ina Eckert
      affiliation:
            name:University of Würzburg
            address:
               name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
               type:PostalAddress
            type:Organization
      name:Eliana Ribechini
      affiliation:
            name:University of Würzburg
            address:
               name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
               type:PostalAddress
            type:Organization
      name:Manfred B. Lutz
      affiliation:
            name:University of Würzburg
            address:
               name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
      name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
      name:Institute for Virology and Immunobiology, University of Würzburg, Würzburg, Germany
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