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Title[redir]:
Transplantation
Description:
nsplantation with myeloid-derived suppressor cells (MDSCs) without requirement of immunosuppression and determine the role of inducible nitric oxide synthase (iNOS) produced by MDSCs in immune regulation. Methods Bone marrow cells were isolated from wild-type (WT) or iNOS−/− mice and cultured in the presence of granulocyte-macrophage colony-stimulating factor and hepatic stellate cells (HSCs), resulting in the generation of MDSCs. WT or iNOS−/− MDSCs were cotransplanted with islet allografts under the renal capsule of diabetic recipient mice. Results Addition of HSCs into DC culture promoted generation of MDSCs (instead of DCs). MDSCs had elevated expression of iNOS upon exposure to IFN-γ and inhibited T-cell responses in an MLR culture. Cotransplantation with WT MDSCs markedly prolonged survival of islet allografts, which was associated with reduced infiltration of CD8+ T cells resulting from inhibited proliferative response. These effects were significantly attenuated when MDSCs were deficient in iNOS. Furthermore, iNOS−/− MDSCs largely lost their ability to protect islet allografts. Conclusions Cotransplantation with HSC-induced MDSCs significantly extends islet allograft survival through iNOS-mediated T-cell inhibition. The results demonstrate the potential use of in vitro generated MDSCs as a novel adjunctive immunotherapy for islet transplantation....
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Keywords {🔍}
mdscs, cells, inos, islet, hscs, tcell, cotransplantation, mice, transplantation, fig, allografts, expression, ifnγ, response, grafts, recipients, immune, dcs, activity, culture, panels, mdsc, survival, isolated, panel, flow, days, addition, proliferative, percentage, cdb, cell, vitro, group, left, graft, analysis, compared, results, cdc, determined, staining, data, journal, ability, hscinduced, liver, inhibit, balbc, immunosuppression,
Topics {✒️}
granulocyte-macrophage colony-stimulating factor fluorochrome-conjugated avidin-biotin system induce cd4+cd25+foxp3+ regulatory th1-cell-derived ifn-γ achieve long-term survival inos-mediated t-cell inhibition induce t-cell apoptosis round-bottom microculture plates inhibit t-cell responses inhibit t-cell response myeloid-derived suppressor cells t-cell proliferative response animal models—liver transplants α-smooth muscle actin extracellular signal-regulated kinase nylon wool–eluted splenic inhibited t-cell responses mdsc-mediated immune suppression administered anti-cd28 mab isotype-matched irrelevant antibodies cd8+ t-cell populations cell-mediated immune response enhanced l-arginine catabolism inos inhibitor l-nmma cd8+ t-cell numbers expressed ova-specific tcr csfe-labeled cd8+ 2c β-cell function mdsc-treated recipients generated mice lacking ifn-γ exerting inhibitory activity dc-treated group elicited t-cell response immature dc [cd11b+cd11c+] islet allograft survival t-cell proliferation t-cell inhibition low proliferative activity inhibit proliferative response hsc-conditioned myeloid inhibit immune response blood glucose level syngeneic hepatocyte transplants t-cell hyporesponsiveness ifn-γ-positive cells flow cytometric analysis hsc-induced mdscs journal authors submit b6 bone marrow significantly prolong survival
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