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         headline:Applications of single-cell sequencing in cancer research: progress and perspectives
         description:Single-cell sequencing, including genomics, transcriptomics, epigenomics, proteomics and metabolomics sequencing, is a powerful tool to decipher the cellular and molecular landscape at a single-cell resolution, unlike bulk sequencing, which provides averaged data. The use of single-cell sequencing in cancer research has revolutionized our understanding of the biological characteristics and dynamics within cancer lesions. In this review, we summarize emerging single-cell sequencing technologies and recent cancer research progress obtained by single-cell sequencing, including information related to the landscapes of malignant cells and immune cells, tumor heterogeneity, circulating tumor cells and the underlying mechanisms of tumor biological behaviors. Overall, the prospects of single-cell sequencing in facilitating diagnosis, targeted therapy and prognostic prediction among a spectrum of tumors are bright. In the near future, advances in single-cell sequencing will undoubtedly improve our understanding of the biological characteristics of tumors and highlight potential precise therapeutic targets for patients.
         datePublished:2021-06-09T00:00:00Z
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      headline:Applications of single-cell sequencing in cancer research: progress and perspectives
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         Heterogeneity
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         Hematology
         Cancer Research
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      name:Fudan University
      address:
         name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
         type:PostalAddress
      name:Fudan University Shanghai Cancer Center
      address:
         name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
         type:PostalAddress
      name:Fudan University
      address:
         name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
         type:PostalAddress
      name:Shanghai Pancreatic Cancer Institute
      address:
         name:Shanghai Pancreatic Cancer Institute, Shanghai, China
         type:PostalAddress
      name:Fudan University
      address:
         name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
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      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Yalan Lei
      affiliation:
            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
               type:PostalAddress
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            name:Shanghai Pancreatic Cancer Institute
            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
      name:Rong Tang
      affiliation:
            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Shanghai Pancreatic Cancer Institute
            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
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            name:Fudan University
            address:
               name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
               type:PostalAddress
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      name:Jin Xu
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            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
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            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
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            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
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            name:Fudan University
            address:
               name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
               type:PostalAddress
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      name:Wei Wang
      affiliation:
            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
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            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
               type:PostalAddress
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            name:Shanghai Pancreatic Cancer Institute
            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
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            address:
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               type:PostalAddress
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      name:Bo Zhang
      affiliation:
            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
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            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
               type:PostalAddress
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            name:Shanghai Pancreatic Cancer Institute
            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
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            name:Fudan University
            address:
               name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
      name:Jiang Liu
      affiliation:
            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Shanghai Pancreatic Cancer Institute
            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
      name:Xianjun Yu
      url:http://orcid.org/0000-0002-6697-7143
      affiliation:
            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Shanghai Pancreatic Cancer Institute
            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Si Shi
      affiliation:
            name:Fudan University Shanghai Cancer Center
            address:
               name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Shanghai Pancreatic Cancer Institute
            address:
               name:Shanghai Pancreatic Cancer Institute, Shanghai, China
               type:PostalAddress
            type:Organization
            name:Fudan University
            address:
               name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China
      name:Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China
      name:Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
      name:Shanghai Pancreatic Cancer Institute, Shanghai, China
      name:Pancreatic Cancer Institute, Fudan University, Shanghai, China

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