We are analyzing https://link.springer.com/article/10.1186/bcr3459.

Title:
Evidence for a multipotent mammary progenitor with pregnancy-specific activity | Breast Cancer Research
Description:
Introduction The mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, our understanding of the mammary developmental hierarchy remains incomplete. Methods We used the MMTV promoter linked to the reverse tetracycline transactivator to induce H2BGFP expression in the mouse mammary gland. Mammary epithelial cells (MECs) from virgin mice were sorted by flow cytometry for expression of the mammary stem cell/progenitor markers CD24 and CD29, and H2BGFP. Sorted populations were analyzed for in vivo repopulation ability, expression of mammary lineage markers, and differential gene expression. Results The reconstituting activity of CD24+/CD29+ cells in cleared fat pad transplantation assays was not distinguished in GFP+ compared to GFP- subpopulations. However, within the CD24+/CD29lo luminal progenitor-enriched population, H2BGFP+, but not H2BGFP-, MECs formed mammary structures in transplantation assays; moreover, this activity was dramatically enhanced in pregnant recipients. These outgrowths contained luminal and myoepithelial mammary lineages and produced milk, but lacked the capacity for serial transplantation. Transcriptional microarray analysis revealed that H2BGFP+/CD24+/CD29lo MECs are distinct from H2BGFP-/CD24+/CD29lo MECs and enriched for gene expression signatures with both the stem cell (CD24+/CD29+) and luminal progenitor (CD24+/CD29lo/CD61+) compartments. Conclusions We have identified a population of MECs containing pregnancy-activated multipotent progenitors that are present in the virgin mammary gland and contribute to the expansion of the mammary gland during pregnancy.
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mammary, cells, hbgfp, stem, expression, mecs, cell, hbgfpcdcdlo, pubmed, article, figure, mice, google, scholar, populations, progenitors, gland, cdcd, data, pregnancy, cas, cdcdlo, population, outgrowths, glands, mouse, luminal, epithelial, mmtvrttahbgfp, expressed, additional, file, analysis, cancer, progenitor, usa, ebioscience, genes, activity, development, gfp, receptor, levels, ability, found, hbgfpcdcd, markers, transgenic, mmtvrtta, fat,

Topics {✒️}

asselin-labat ml 1 μg/ml dapi h2bgfp-/cd24+/cd29lo mecs segregated long-label-retaining mammary cells cd24+/cd29lo/cd61- mature cells apparent h2bgfp-/cd24+/cd29+ outgrowths article download pdf h2bgfp+/cd24+/cd29lo mecs produced single-hit poisson assumption fluorescence-activated cell sorting matrigel-dependent outgrowth-generating ability label-retaining epithelial cells h2bgfp-/cd24+/cd29+-derived glands rosa26-lox-lacz allele live label-retaining cells mammary stem cell/progenitor mammary stem-cell enriched mammary stem cell-enriched cd24+/cd29lo cells possessing half-grown mammary gland h2gfp-/cd24e/cd29lo cells mammary stem cells/progenitors t-cell receptor delta separating h2bgfp+/cd24+/cd29lo cd24+/cd29lo compartment labels cd24e/cd29lo/cd61+ population inverted y-shaped incision h2gfp+/cd24+/cd29lo cells mmtvrtta/h2bgfp label retention h2bgfp+/cd24+/cd29lo cells h2bgfp-/cd24+/cd29lo cells cd24+/cd29lo/cd61+ mecs cd24+/cd29lo/cd61- mecs h2bgfp+/cd24+/cd29e cells pregnancy-activated progenitors remains gfp- cd24+/cd29lo cells cd24+/cd29lo/cd61+ subpopulations cd24+/cd29lo/cd61+ populations cd24+/cd29e/cd61+ subpopulations breast cancer research h2bgfp+/cd24+/cd29lo mecs h2bgfp-/cd24+/cd29lo mecs h2bgfp+ cd24+/cd29lo mecs h2gfp- cd24+/cd29lo populations pregnancy-activated multipotent progenitor mmtvrtta transgene-induced expression h2bgfp+/cd24+/cd29+ cells h2bgfp-/cd24+/cd29+ cells h2bgfp+/cd24+/cd29- cells pregnancy-activated progenitors conflicts

Questions {❓}

Vaillant F, Lindeman GJ, Visvader JE: Jekyll or Hyde: does Matrigel provide a more or less physiological environment in mammary repopulating assays?

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WebPage:
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         headline:Evidence for a multipotent mammary progenitor with pregnancy-specific activity
         description:The mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, our understanding of the mammary developmental hierarchy remains incomplete. We used the MMTV promoter linked to the reverse tetracycline transactivator to induce H2BGFP expression in the mouse mammary gland. Mammary epithelial cells (MECs) from virgin mice were sorted by flow cytometry for expression of the mammary stem cell/progenitor markers CD24 and CD29, and H2BGFP. Sorted populations were analyzed for in vivo repopulation ability, expression of mammary lineage markers, and differential gene expression. The reconstituting activity of CD24+/CD29+ cells in cleared fat pad transplantation assays was not distinguished in GFP+ compared to GFP- subpopulations. However, within the CD24+/CD29lo luminal progenitor-enriched population, H2BGFP+, but not H2BGFP-, MECs formed mammary structures in transplantation assays; moreover, this activity was dramatically enhanced in pregnant recipients. These outgrowths contained luminal and myoepithelial mammary lineages and produced milk, but lacked the capacity for serial transplantation. Transcriptional microarray analysis revealed that H2BGFP+/CD24+/CD29lo MECs are distinct from H2BGFP-/CD24+/CD29lo MECs and enriched for gene expression signatures with both the stem cell (CD24+/CD29+) and luminal progenitor (CD24+/CD29lo/CD61+) compartments. We have identified a population of MECs containing pregnancy-activated multipotent progenitors that are present in the virgin mammary gland and contribute to the expansion of the mammary gland during pregnancy.
         datePublished:2013-08-15T00:00:00Z
         dateModified:2013-08-15T00:00:00Z
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            Mammary stem cells
            Pregnancy
            MMTVrtTA
            H2BGFP
            Cancer Research
            Oncology
            Surgical Oncology
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ScholarlyArticle:
      headline:Evidence for a multipotent mammary progenitor with pregnancy-specific activity
      description:The mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, our understanding of the mammary developmental hierarchy remains incomplete. We used the MMTV promoter linked to the reverse tetracycline transactivator to induce H2BGFP expression in the mouse mammary gland. Mammary epithelial cells (MECs) from virgin mice were sorted by flow cytometry for expression of the mammary stem cell/progenitor markers CD24 and CD29, and H2BGFP. Sorted populations were analyzed for in vivo repopulation ability, expression of mammary lineage markers, and differential gene expression. The reconstituting activity of CD24+/CD29+ cells in cleared fat pad transplantation assays was not distinguished in GFP+ compared to GFP- subpopulations. However, within the CD24+/CD29lo luminal progenitor-enriched population, H2BGFP+, but not H2BGFP-, MECs formed mammary structures in transplantation assays; moreover, this activity was dramatically enhanced in pregnant recipients. These outgrowths contained luminal and myoepithelial mammary lineages and produced milk, but lacked the capacity for serial transplantation. Transcriptional microarray analysis revealed that H2BGFP+/CD24+/CD29lo MECs are distinct from H2BGFP-/CD24+/CD29lo MECs and enriched for gene expression signatures with both the stem cell (CD24+/CD29+) and luminal progenitor (CD24+/CD29lo/CD61+) compartments. We have identified a population of MECs containing pregnancy-activated multipotent progenitors that are present in the virgin mammary gland and contribute to the expansion of the mammary gland during pregnancy.
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         Mammary stem cells
         Pregnancy
         MMTVrtTA
         H2BGFP
         Cancer Research
         Oncology
         Surgical Oncology
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            name:Harvard Medical School
            address:
               name:Department of Cell Biology, Harvard Medical School, Boston, USA
               type:PostalAddress
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      email:[email protected]
      name:Benjamin G Neel
      affiliation:
            name:Beth Israel Deaconess Medical Center, Harvard Medical School
            address:
               name:Department of Medicine, Cancer Biology Program, Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA
               type:PostalAddress
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               type:PostalAddress
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PostalAddress:
      name:Department of Medicine, Cancer Biology Program, Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA
      name:Department of Cell Biology, Harvard Medical School, Boston, USA
      name:Campbell Family Cancer Research Institute, Ontario Cancer Institute, Princess Margaret Cancer Center, University Health Network, Toronto, Canada
      name:Department of Cell Biology, Harvard Medical School, Boston, USA
      name:Department of Cell Biology, Harvard Medical School, Boston, USA
      name:Department of Medicine, Cancer Biology Program, Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA
      name:Campbell Family Cancer Research Institute, Ontario Cancer Institute, Princess Margaret Cancer Center, University Health Network, Toronto, Canada
      name:Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Canada

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