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Keywords {🔍}

cells, matrigel, doi, mammary, cell, stem, luminal, pubmed, google, scholar, transplantation, outgrowths, pmc, cancer, activity, article, progenitor, subset, shown, free, nlm, breast, repopulating, assays, matrix, population, capacity, mascs, subpopulation, data, human, growth, nature, ncbi, search, vaillant, lindeman, visvader, vivo, mouse, fat, epithelial, mascenriched, glands, mice, masc, studies, transplanted, selfrenewal, subpopulations,

Topics {✒️}

engelbreth-holm-swarm mouse sarcoma fvb/n-rosa26 female mice pmc beta search enhance tumor-initiating capacity current 'gold-standard' assay luminal cell-fate commitment x-gal-stained outgrowths references daniel cw license information pmcid publisher site pdf growth factor-reduced matrigel breast cancer res rare bipotent cells moraes rc mouse sarcoma line breast cancer research human breast cancers human breast carcinomas reconstituted basement membrane competing interests cell stem cell mammary stem cell functional mammary gland transplanted double-sorted cells human breast tissue mammary repopulating frequency squamous cell carcinomas luminal cell-derived single stem cell x-gal staining small branched structures mammary epithelial outgrowths tumor stem cells masc-enriched basal population stem cell activity β-galactosidase activity mammary stem cells luminal progenitor cells single cell suspensions bona fide mascs fat pads transplanted mammary fat pad luminal cell subset cancer stem cells mammary repopulating assays pmc disclaimer unsorted mammary cells aberrant luminal progenitors abbreviations masc increased repopulating capacity

Questions {❓}

• Context, tissue plasticity, and cancer: are tumor stem cells also regulated by the microenvironment?
• Jekyll or Hyde: does Matrigel provide a more or less physiological environment in mammary repopulating assays?

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