We are analyzing https://link.springer.com/article/10.1007/s13258-021-01055-6.

Title:
Transglutaminase 2 mediates transcriptional regulation through BAF250a polyamination | Genes & Genomics
Description:
Background Transglutaminase 2 (TG2) mediates protein modifications by crosslinking or by incorporating polyamine in response to oxidative or DNA-damaging stress, thereby regulating apoptosis, extracellular matrix formation, and inflammation. The regulation of transcriptional activity by TG2-mediated histone serotonylation or by Sp1 crosslinking may also contribute to cellular stress responses. Objective In this study, we attempted to identify TG2-interacting proteins to better understand the role of TG2 in transcriptional regulation. Methods Using a yeast two-hybrid assay to screen a HeLa cell cDNA library, we found that TG2 bound BAF250a, a core subunit of the cBAF chromatin remodeling complex, through an interaction between the TG2 barrel 1 and BAF250a C-terminal domains. Results TG2 was pulled down with a GST-BAF250a C-term fusion protein. Moreover, TG2 and BAF250a were co-fractionated using P11 chromatography, and co-immunoprecipitated. A transamidation reaction showed that TG2 mediated incorporation of polyamine into BAF250a. In glucocorticoid response-element reporter-expressing cells, TG2 overexpression increased the luciferase reporter activity in a transamidation-dependent manner. In addition, a comparison of genome-wide gene expression between wild-type and TG2-deficient primary hepatocytes in response to dexamethasone treatment showed that TG2 further enhanced or suppressed the expression of dexamethasone-regulated genes that were identified by a gene ontology enrichment analysis. Conclusion Thus, our results indicate that TG2 regulates transcriptional activity through BAF250a polyamination.
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Keywords {🔍}

pubmed, article, google, scholar, cas, transglutaminase, central, cell, transcriptional, bafa, kim, gene, biol, complex, regulation, lee, jeon, activity, access, death, mol, swisnf, seoul, privacy, cookies, content, research, mediates, polyamination, cho, ingyu, protein, stress, inflammation, chromatin, remodeling, human, chem, nature, korea, data, information, publish, search, genes, cells, transcription, cancer, sci, biology,

Topics {✒️}

month download article/chapter swi/snf chromatin remodeling brahma chromatin-remodeling complex genome-wide gene expression baf250a c-terminal domains mediates protein modifications cell type-specific activation transglutaminase gene family identify tg2-interacting proteins transcription factor sp1 enhances tfiid binding gh/tissue transglutaminase ii human-environment interface biology full article pdf tg2-deficient primary hepatocytes national research foundation swi/snf complex ju-hong jeon ju-hong jeon drosophila osa gene multi-tasking protein tg2-mediated histone serotonylation dexamethasone-regulated genes related subjects age-related cataractogenesis transcriptional regulation selenite-induced cataract privacy choices/manage cookies transamidation reaction showed cellular stress responses luciferase reporter activity jin-haeng lee sung-yup cho sung-yup cho baf remodeling complex glucocorticoid receptor requires article kim dna-damaging stress transamidation-dependent manner dexamethasone treatment showed hypoxic tumor cells dexamethasone treatment increases potential molecular marker transcriptional activity transglutaminase 2 gene life sci alliance human papillomavirus e7 arm-repeat domains nf-kappab activity european economic area

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         description:Transglutaminase 2 (TG2) mediates protein modifications by crosslinking or by incorporating polyamine in response to oxidative or DNA-damaging stress, thereby regulating apoptosis, extracellular matrix formation, and inflammation. The regulation of transcriptional activity by TG2-mediated histone serotonylation or by Sp1 crosslinking may also contribute to cellular stress responses. In this study, we attempted to identify TG2-interacting proteins to better understand the role of TG2 in transcriptional regulation. Using a yeast two-hybrid assay to screen a HeLa cell cDNA library, we found that TG2 bound BAF250a, a core subunit of the cBAF chromatin remodeling complex, through an interaction between the TG2 barrel 1 and BAF250a C-terminal domains. TG2 was pulled down with a GST-BAF250a C-term fusion protein. Moreover, TG2 and BAF250a were co-fractionated using P11 chromatography, and co-immunoprecipitated. A transamidation reaction showed that TG2 mediated incorporation of polyamine into BAF250a. In glucocorticoid response-element reporter-expressing cells, TG2 overexpression increased the luciferase reporter activity in a transamidation-dependent manner. In addition, a comparison of genome-wide gene expression between wild-type and TG2-deficient primary hepatocytes in response to dexamethasone treatment showed that TG2 further enhanced or suppressed the expression of dexamethasone-regulated genes that were identified by a gene ontology enrichment analysis. Thus, our results indicate that TG2 regulates transcriptional activity through BAF250a polyamination.
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            BAF250a
            Transamidation
            Dexamethasone
            Transcriptional activity
            Microbial Genetics and Genomics
            Plant Genetics and Genomics
            Animal Genetics and Genomics
            Human Genetics
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      description:Transglutaminase 2 (TG2) mediates protein modifications by crosslinking or by incorporating polyamine in response to oxidative or DNA-damaging stress, thereby regulating apoptosis, extracellular matrix formation, and inflammation. The regulation of transcriptional activity by TG2-mediated histone serotonylation or by Sp1 crosslinking may also contribute to cellular stress responses. In this study, we attempted to identify TG2-interacting proteins to better understand the role of TG2 in transcriptional regulation. Using a yeast two-hybrid assay to screen a HeLa cell cDNA library, we found that TG2 bound BAF250a, a core subunit of the cBAF chromatin remodeling complex, through an interaction between the TG2 barrel 1 and BAF250a C-terminal domains. TG2 was pulled down with a GST-BAF250a C-term fusion protein. Moreover, TG2 and BAF250a were co-fractionated using P11 chromatography, and co-immunoprecipitated. A transamidation reaction showed that TG2 mediated incorporation of polyamine into BAF250a. In glucocorticoid response-element reporter-expressing cells, TG2 overexpression increased the luciferase reporter activity in a transamidation-dependent manner. In addition, a comparison of genome-wide gene expression between wild-type and TG2-deficient primary hepatocytes in response to dexamethasone treatment showed that TG2 further enhanced or suppressed the expression of dexamethasone-regulated genes that were identified by a gene ontology enrichment analysis. Thus, our results indicate that TG2 regulates transcriptional activity through BAF250a polyamination.
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         Transglutaminase 2 (TG2)
         BAF250a
         Transamidation
         Dexamethasone
         Transcriptional activity
         Microbial Genetics and Genomics
         Plant Genetics and Genomics
         Animal Genetics and Genomics
         Human Genetics
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