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We began analyzing https://journals.lww.com/hep/abstract/1997/12000/an_improved_digitonin_collagenase_perfusion.31.aspx, but it redirected us to https://journals.lww.com/hep/abstract/1997/12000/an_improved_digitonin_collagenase_perfusion.31.aspx. The analysis below is for the second page.

Title[redir]:
Hepatology
Description:
portal (PP) or perivenous (PV) hepatocytes are isolated from different livers, allowing only comparison between cell populations issued from two separate animals. To overcome this drawback, we have modified this technique by perfusing the two main rat liver lobes of a single animal in succession. The procedure involved alternate clamping of the median and the left lateral lobes, restricting digitonin infusion to one lobe via the portal vein, and to the other via the caudal vena cava. Lobe exclusion during digitonin perfusion, and zonal restriction of digitonin-induced damage, were monitored using macroscopic and histological controls. We compared our results with previous data on PP and PV hepatocytes issued from two different livers using the conventional digitonin-collagenase perfusion technique. First, we found that the cellular sensitivity to angiotensin II, a calcium-mobilizing agonist, was 60% to 80% higher in PV than in PP hepatocytes, whereas, previously, no difference had been recorded. Second, we found that albumin messenger RNAs (mRNAs) were 35% more abundant in PP than in PV hepatocytes, whereas, previously, larger differences had been reported. Our results show that PP and PV hepatocytes may be isolated from a single liver using an improved digitonin-collagenase perfusion technique. Furthermore, we suggest that zonal differences can be artificially masked or amplified when comparing PP and PV cell populations from two different livers, indicating that it is preferable to use a single liver for accurate zonal comparisons between hepatocytes. Copyright © 1997 American Association for the Study of Liver Diseases....

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🌍 Impressive Traffic: 500k - 1M visitors per month


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Keywords {🔍}

liver, hepatocytes, aasld, perfusion, technique, log, journal, digitonincollagenase, single, access, hepatology, subscribe, issue, alerts, abstract, heterogeneity, study, data, livers, zonal, register, member, logo, articles, browse, submit, previous, improved, periportal, perivenous, rat, buy, procedure, isolated, cell, populations, issued, lobes, digitonin, lobe, results, found, previously, differences, copyright, american, association, diseases, text, subscribers,

Topics {✒️}

digitonin-collagenase perfusion technique aasld members log open access policy single rat liver individual subscribers log restricting digitonin infusion digitonin-induced damage left lateral lobes institutional users access cell populations issued caudal vena cava calcium-mobilizing agonist albumin messenger rnas artificial intelligence training yielded reliable data accurate zonal comparisons full text access liver diseases digitonin perfusion pv cell populations study hepatocyte heterogeneity single liver pv hepatocytes issued journal tables liver lobule register subscribe service request 800-638-3030 previous data journal functional heterogeneity website subscribe zonal restriction zonal differences data mining single animal physiological implications separate animals technique portal vein histological controls cellular sensitivity angiotensin ii larger differences artificially masked email inbox rights reserved pv hepatocytes issue lobe exclusion results show

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