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  1. Analyzed Page
  2. Matching Content Categories
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  4. Monthly Traffic Estimate
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  6. Keywords
  7. Topics
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We are analyzing https://link.springer.com/article/10.1007/s12307-012-0121-z.

Title:
Transcription Factor Networks in Invasion-Promoting Breast Carcinoma-Associated Fibroblasts | Cancer Microenvironment
Description:
Carcinoma-associated fibroblasts (CAFs) contribute to both tumor growth and cancer progression. In this report, we applied an emerging transcription factor (TF) activity array to fibroblasts to capture the activity of the intracellular signaling network and to define a signature that distinguishes mammary CAFs from normal mammary fibroblasts. Normal fibroblasts that restrained cancer cell invasion developed into an invasion-promoting CAF phenotype through exposure to conditioned medium from MDA-MB-231 breast cancer cells. A myofibroblast-like CAF cell line expressing high levels of smooth muscle actin was compared to normal mammary fibroblasts before and after induction. Comparison of TF activity profiles for all three fibroblast types identified a TF activity signature common to CAFs which included activation of reporters for TFs ELK1, GATA1, retinoic acid receptor (RAR), serum response factor (SRF), and vitamin D receptor (VDR). Additionally, CAFs resembling myofibroblasts, relative to normal fibroblasts, had elevated activation corresponding to NF-kappaB, RUNX2, and YY1, and distinct activity patterns for several differentiation-related TF reporters. Induction of CAFs by exposure of normal fibroblasts to conditioned medium from MDA-MB-231 cells resulted in increased activation of reporters for HIF1, several STAT TFs, and proliferation-related TFs such as AP1. Myofibroblast-like CAFs and induced normal mammary fibroblasts promoted invasion of breast cancer cells by distinct mechanisms, consistent with their distinct patterns of TF activation. The TF activity profiles of CAF subtypes provide an overview of intracellular signaling associated with the induction of a pro-invasive stroma, and provide a mechanistic link between the microenvironmental stimuli and phenotypic response.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {πŸ“š}

  • Education
  • Science
  • Health & Fitness

Content Management System {πŸ“}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {πŸ“ˆ}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {πŸ’Έ}

We find it hard to spot revenue streams.

Not all websites focus on profit; some are designed to educate, connect people, or share useful tools. People create websites for numerous reasons. And this could be one such example. Link.springer.com might be making money, but it's not detectable how they're doing it.

Keywords {πŸ”}

article, pubmed, google, scholar, cancer, cas, breast, fibroblasts, cell, stromal, tumor, cells, expression, normal, van, microenvironment, biol, human, shea, activity, res, transcription, factor, mammary, fibroblast, factors, usa, carcinomaassociated, stroma, rijn, gene, chicago, northwestern, university, analysis, jeruss, cafs, invasion, induction, activation, access, molecular, invasive, sci, brown, heterogeneity, privacy, cookies, content, growth,

Topics {βœ’οΈ}

nf-kappab-yy1-mir-29 regulatory circuitry nf-kappab-dependent manner month download article/chapter transcription factor networks stromal cell-derived factor-1 invasion-promoting caf phenotype invasion-promoting breast carcinoma orchestrate tumor-promoting inflammation mda-mb-231 cells resulted emerging transcription factor transcription factor activity fibroblast activation protein/seprase nf-kappab caveolin-1 predicts outcome tumor cell invasion article cancer microenvironment elevated sdf-1/cxcl12 secretion serum response factor full article pdf related subjects human breast carcinomas differentiation-related tf reporters smooth muscle actin worse clinical outcome tumour biol autocrine tgf-beta gene expression signature privacy choices/manage cookies retinoic acid receptor matrix metalloproteinase-1 proliferation-related tfs transcription factors gene expression programs stromal cell expression van de vijver fibroblast secreted factors martinez-outschoorn ue tumor stromal microenvironment invasive cancer growth invasive breast cancer human breast tumours modular cell biology cell type specificity tumor-stroma interactions receptor tyrosine kinases grant number pl1eb00854 invasive breast lesions cancer-stroma interactions tumor cells induce invasive breast carcinoma

Schema {πŸ—ΊοΈ}

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         headline:Transcription Factor Networks in Invasion-Promoting Breast Carcinoma-Associated Fibroblasts
         description:Carcinoma-associated fibroblasts (CAFs) contribute to both tumor growth and cancer progression. In this report, we applied an emerging transcription factor (TF) activity array to fibroblasts to capture the activity of the intracellular signaling network and to define a signature that distinguishes mammary CAFs from normal mammary fibroblasts. Normal fibroblasts that restrained cancer cell invasion developed into an invasion-promoting CAF phenotype through exposure to conditioned medium from MDA-MB-231 breast cancer cells. A myofibroblast-like CAF cell line expressing high levels of smooth muscle actin was compared to normal mammary fibroblasts before and after induction. Comparison of TF activity profiles for all three fibroblast types identified a TF activity signature common to CAFs which included activation of reporters for TFs ELK1, GATA1, retinoic acid receptor (RAR), serum response factor (SRF), and vitamin D receptor (VDR). Additionally, CAFs resembling myofibroblasts, relative to normal fibroblasts, had elevated activation corresponding to NF-kappaB, RUNX2, and YY1, and distinct activity patterns for several differentiation-related TF reporters. Induction of CAFs by exposure of normal fibroblasts to conditioned medium from MDA-MB-231 cells resulted in increased activation of reporters for HIF1, several STAT TFs, and proliferation-related TFs such as AP1. Myofibroblast-like CAFs and induced normal mammary fibroblasts promoted invasion of breast cancer cells by distinct mechanisms, consistent with their distinct patterns of TF activation. The TF activity profiles of CAF subtypes provide an overview of intracellular signaling associated with the induction of a pro-invasive stroma, and provide a mechanistic link between the microenvironmental stimuli and phenotypic response.
         datePublished:2012-10-23T00:00:00Z
         dateModified:2012-10-23T00:00:00Z
         pageStart:91
         pageEnd:107
         sameAs:https://doi.org/10.1007/s12307-012-0121-z
         keywords:
            Carcinoma-associated fibroblasts
            Breast cancer
            Transcription factors
            Gene regulation
            Induction
            Cancer Research
            Oncology
            Immunology
            Cell Biology
            Biochemistry
            general
            Biomedicine
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                     address:
                        name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
                        type:PostalAddress
                     type:Organization
                     name:Northwestern University
                     address:
                        name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
                        type:PostalAddress
                     type:Organization
                     name:Northwestern University
                     address:
                        name:Institute for BioNanotechnology in Medicine (IBNAM), Northwestern University, Chicago, USA
                        type:PostalAddress
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                     name:Northwestern University
                     address:
                        name:Chemistry of Life Processes Institute (CLP), Northwestern University, Evanston, USA
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      headline:Transcription Factor Networks in Invasion-Promoting Breast Carcinoma-Associated Fibroblasts
      description:Carcinoma-associated fibroblasts (CAFs) contribute to both tumor growth and cancer progression. In this report, we applied an emerging transcription factor (TF) activity array to fibroblasts to capture the activity of the intracellular signaling network and to define a signature that distinguishes mammary CAFs from normal mammary fibroblasts. Normal fibroblasts that restrained cancer cell invasion developed into an invasion-promoting CAF phenotype through exposure to conditioned medium from MDA-MB-231 breast cancer cells. A myofibroblast-like CAF cell line expressing high levels of smooth muscle actin was compared to normal mammary fibroblasts before and after induction. Comparison of TF activity profiles for all three fibroblast types identified a TF activity signature common to CAFs which included activation of reporters for TFs ELK1, GATA1, retinoic acid receptor (RAR), serum response factor (SRF), and vitamin D receptor (VDR). Additionally, CAFs resembling myofibroblasts, relative to normal fibroblasts, had elevated activation corresponding to NF-kappaB, RUNX2, and YY1, and distinct activity patterns for several differentiation-related TF reporters. Induction of CAFs by exposure of normal fibroblasts to conditioned medium from MDA-MB-231 cells resulted in increased activation of reporters for HIF1, several STAT TFs, and proliferation-related TFs such as AP1. Myofibroblast-like CAFs and induced normal mammary fibroblasts promoted invasion of breast cancer cells by distinct mechanisms, consistent with their distinct patterns of TF activation. The TF activity profiles of CAF subtypes provide an overview of intracellular signaling associated with the induction of a pro-invasive stroma, and provide a mechanistic link between the microenvironmental stimuli and phenotypic response.
      datePublished:2012-10-23T00:00:00Z
      dateModified:2012-10-23T00:00:00Z
      pageStart:91
      pageEnd:107
      sameAs:https://doi.org/10.1007/s12307-012-0121-z
      keywords:
         Carcinoma-associated fibroblasts
         Breast cancer
         Transcription factors
         Gene regulation
         Induction
         Cancer Research
         Oncology
         Immunology
         Cell Biology
         Biochemistry
         general
         Biomedicine
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                  name:Northwestern University
                  address:
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                     type:PostalAddress
                  type:Organization
                  name:Northwestern University Feinberg School of Medicine
                  address:
                     name:Medical Scientist Training Program, Northwestern University Feinberg School of Medicine, Chicago, USA
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                  type:Organization
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            name:E. Kniazeva
            affiliation:
                  name:Northwestern University
                  address:
                     name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:J. S. Jeruss
            affiliation:
                  name:Northwestern University Feinberg School of Medicine
                  address:
                     name:Department of Surgery, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, USA
                     type:PostalAddress
                  type:Organization
                  name:Northwestern University
                  address:
                     name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:L. D. Shea
            affiliation:
                  name:Northwestern University
                  address:
                     name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
                     type:PostalAddress
                  type:Organization
                  name:Northwestern University
                  address:
                     name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
                     type:PostalAddress
                  type:Organization
                  name:Northwestern University
                  address:
                     name:Institute for BioNanotechnology in Medicine (IBNAM), Northwestern University, Chicago, USA
                     type:PostalAddress
                  type:Organization
                  name:Northwestern University
                  address:
                     name:Chemistry of Life Processes Institute (CLP), Northwestern University, Evanston, USA
                     type:PostalAddress
                  type:Organization
                  name:Northwestern University
                  address:
                     name:Department of Chemical and Biological Engineering, Northwestern University, Evanston, USA
                     type:PostalAddress
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      name:Northwestern University
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         name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
         type:PostalAddress
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      address:
         name:Medical Scientist Training Program, Northwestern University Feinberg School of Medicine, Chicago, USA
         type:PostalAddress
      name:Northwestern University
      address:
         name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
         type:PostalAddress
      name:Northwestern University Feinberg School of Medicine
      address:
         name:Department of Surgery, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, USA
         type:PostalAddress
      name:Northwestern University
      address:
         name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
         type:PostalAddress
      name:Northwestern University
      address:
         name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
         type:PostalAddress
      name:Northwestern University
      address:
         name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
         type:PostalAddress
      name:Northwestern University
      address:
         name:Institute for BioNanotechnology in Medicine (IBNAM), Northwestern University, Chicago, USA
         type:PostalAddress
      name:Northwestern University
      address:
         name:Chemistry of Life Processes Institute (CLP), Northwestern University, Evanston, USA
         type:PostalAddress
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      affiliation:
            name:Northwestern University
            address:
               name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
               type:PostalAddress
            type:Organization
            name:Northwestern University Feinberg School of Medicine
            address:
               name:Medical Scientist Training Program, Northwestern University Feinberg School of Medicine, Chicago, USA
               type:PostalAddress
            type:Organization
      name:E. Kniazeva
      affiliation:
            name:Northwestern University
            address:
               name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
               type:PostalAddress
            type:Organization
      name:J. S. Jeruss
      affiliation:
            name:Northwestern University Feinberg School of Medicine
            address:
               name:Department of Surgery, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, USA
               type:PostalAddress
            type:Organization
            name:Northwestern University
            address:
               name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
               type:PostalAddress
            type:Organization
      name:L. D. Shea
      affiliation:
            name:Northwestern University
            address:
               name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
               type:PostalAddress
            type:Organization
            name:Northwestern University
            address:
               name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
               type:PostalAddress
            type:Organization
            name:Northwestern University
            address:
               name:Institute for BioNanotechnology in Medicine (IBNAM), Northwestern University, Chicago, USA
               type:PostalAddress
            type:Organization
            name:Northwestern University
            address:
               name:Chemistry of Life Processes Institute (CLP), Northwestern University, Evanston, USA
               type:PostalAddress
            type:Organization
            name:Northwestern University
            address:
               name:Department of Chemical and Biological Engineering, Northwestern University, Evanston, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
      name:Medical Scientist Training Program, Northwestern University Feinberg School of Medicine, Chicago, USA
      name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
      name:Department of Surgery, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, USA
      name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
      name:Department of Chemical and Biological Engineering, McCormick School of Engineering, Northwestern University, Evanston, USA
      name:Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, USA
      name:Institute for BioNanotechnology in Medicine (IBNAM), Northwestern University, Chicago, USA
      name:Chemistry of Life Processes Institute (CLP), Northwestern University, Evanston, USA
      name:Department of Chemical and Biological Engineering, Northwestern University, Evanston, USA
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