We are analyzing https://link.springer.com/article/10.1007/s12033-024-01256-w.

Title:
Enhanced SELEX Platforms for Aptamer Selection with Improved Characteristics: A Review | Molecular Biotechnology
Description:
This review delves into the advancements in molecular recognition through enhanced SELEX (Systematic Evolution of Ligands by Exponential Enrichment) platforms and post-aptamer modifications. Aptamers, with their superior specificity and affinity compared to antibodies, are central to this discussion. Despite the advantages of the SELEX process—encompassing stages like ssDNA library preparation, incubation, separation, and PCR amplification—it faces challenges, such as nuclease susceptibility. To address these issues and propel aptamer technology forward, we examine next-generation SELEX platforms, including microfluidic-based SELEX, capillary electrophoresis SELEX, cell-based aptamer selection, counter-SELEX, in vivo SELEX, and high-throughput sequencing SELEX, highlighting their respective merits and innovations. Furthermore, this article underscores the significance of post-aptamer modifications, particularly chemical strategies that enhance aptamer stability, reduce renal filtration, and expand their target range, thereby broadening their utility in diagnostics, therapeutics, and nanotechnology. By synthesizing these advanced SELEX platforms and modifications, this review illuminates the dynamic progress in aptamer research and outlines the ongoing efforts to surmount existing challenges and enhance their clinical applicability, charting a path for future breakthroughs in this evolving field.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Science
Education
Shopping

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

While many websites aim to make money, others are created to share knowledge or showcase creativity. People build websites for various reasons. This could be one of them. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

pubmed, article, google, scholar, cas, aptamers, central, selection, selex, aptamer, chemistry, dna, nucleic, molecular, rna, journal, research, chemical, nature, cancer, acid, acids, sciences, analytical, biology, biotechnology, review, modifications, therapeutics, platforms, ligands, molecules, microfluidic, reviews, ankara, privacy, cookies, content, information, evolution, capillary, applications, proceedings, national, academy, engineering, current, medicine, bowser, materials,

Topics {✒️}

2′-deoxy-2′-fluoro-d-arabinonucleic acids nimet yildirim-tirgil month download article/chapter nanoporous sol-gel arrays cell-specific aptamer probes aqueous sol−gel process aptamer-based biosensors oligodeoxyribonucleotide-polyethylene glycol conjugates g-rich oligonucleotide as1411 g-quadruplex induced stabilization generation selex platforms selex process—encompassing stages post-aptamer modifications including microfluidic-based selex cell-based aptamer selection tumor-targeting rna motifs gnrh binding rna small-molecule porphyrin target rna aptamer-based therapeutics enhanced selex platforms high-throughput sequencing selex molecular therapy-nucleic acids full article pdf membrane protein elucidation post-selex optimization effective molecular probes capillary electrophoresis–selex selection capillary electrophoresis-selex selection advanced selex platforms sol–gel materials privacy choices/manage cookies cell-based selection article didarian targeted drug delivery yıldırım beyazıt university nucleic acids research tumor cell selex cell-selex identifies related subjects hiv-1 reverse transcriptase high-throughput sequencing differential inhibitory activities accepted manuscript version technological research council rna aptamer selection pegylated rna library rna pseudoknot inhibitor unlocked nucleic acids improving aptamer performance capillary electrophoresis selex

Questions {❓}

Aptamers as therapeutic agents: Has the initial euphoria subsided?
Aptamers: Novel molecules as diagnostic markers in bacterial and viral infections?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Enhanced SELEX Platforms for Aptamer Selection with Improved Characteristics: A Review
         description:This review delves into the advancements in molecular recognition through enhanced SELEX (Systematic Evolution of Ligands by Exponential Enrichment) platforms and post-aptamer modifications. Aptamers, with their superior specificity and affinity compared to antibodies, are central to this discussion. Despite the advantages of the SELEX process—encompassing stages like ssDNA library preparation, incubation, separation, and PCR amplification—it faces challenges, such as nuclease susceptibility. To address these issues and propel aptamer technology forward, we examine next-generation SELEX platforms, including microfluidic-based SELEX, capillary electrophoresis SELEX, cell-based aptamer selection, counter-SELEX, in vivo SELEX, and high-throughput sequencing SELEX, highlighting their respective merits and innovations. Furthermore, this article underscores the significance of post-aptamer modifications, particularly chemical strategies that enhance aptamer stability, reduce renal filtration, and expand their target range, thereby broadening their utility in diagnostics, therapeutics, and nanotechnology. By synthesizing these advanced SELEX platforms and modifications, this review illuminates the dynamic progress in aptamer research and outlines the ongoing efforts to surmount existing challenges and enhance their clinical applicability, charting a path for future breakthroughs in this evolving field.
         datePublished:2024-08-16T00:00:00Z
         dateModified:2024-08-16T00:00:00Z
         pageStart:1
         pageEnd:16
         sameAs:https://doi.org/10.1007/s12033-024-01256-w
         keywords:
            Aptamers
            SELEX
            Chemical modifications
            Post-aptamer modifications
            Next-generation SELEX platforms
            Biotechnology
            Biochemistry
            general
            Cell Biology
            Protein Science
            Biological Techniques
            Human Genetics
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig5_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig6_HTML.png
         isPartOf:
            name:Molecular Biotechnology
            issn:
               1559-0305
               1073-6085
            type:
               Periodical
         publisher:
            name:Springer US
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Reza Didarian
               affiliation:
                     name:Ankara Yıldırım Beyazıt University
                     address:
                        name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Hatice K. Ozbek
               affiliation:
                     name:Ankara Yıldırım Beyazıt University
                     address:
                        name:Metallurgical and Materials Engineering Department, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Veli C. Ozalp
               affiliation:
                     name:Atilim University
                     address:
                        name:Department of Medical Biology, School of Medicine, Atilim University, Ankara, Türkiye
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ozcan Erel
               affiliation:
                     name:Yıldırım Beyazıt University
                     address:
                        name:Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Türkiye
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Nimet Yildirim-Tirgil
               url:http://orcid.org/0000-0002-5973-8830
               affiliation:
                     name:Ankara Yıldırım Beyazıt University
                     address:
                        name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Enhanced SELEX Platforms for Aptamer Selection with Improved Characteristics: A Review
      description:This review delves into the advancements in molecular recognition through enhanced SELEX (Systematic Evolution of Ligands by Exponential Enrichment) platforms and post-aptamer modifications. Aptamers, with their superior specificity and affinity compared to antibodies, are central to this discussion. Despite the advantages of the SELEX process—encompassing stages like ssDNA library preparation, incubation, separation, and PCR amplification—it faces challenges, such as nuclease susceptibility. To address these issues and propel aptamer technology forward, we examine next-generation SELEX platforms, including microfluidic-based SELEX, capillary electrophoresis SELEX, cell-based aptamer selection, counter-SELEX, in vivo SELEX, and high-throughput sequencing SELEX, highlighting their respective merits and innovations. Furthermore, this article underscores the significance of post-aptamer modifications, particularly chemical strategies that enhance aptamer stability, reduce renal filtration, and expand their target range, thereby broadening their utility in diagnostics, therapeutics, and nanotechnology. By synthesizing these advanced SELEX platforms and modifications, this review illuminates the dynamic progress in aptamer research and outlines the ongoing efforts to surmount existing challenges and enhance their clinical applicability, charting a path for future breakthroughs in this evolving field.
      datePublished:2024-08-16T00:00:00Z
      dateModified:2024-08-16T00:00:00Z
      pageStart:1
      pageEnd:16
      sameAs:https://doi.org/10.1007/s12033-024-01256-w
      keywords:
         Aptamers
         SELEX
         Chemical modifications
         Post-aptamer modifications
         Next-generation SELEX platforms
         Biotechnology
         Biochemistry
         general
         Cell Biology
         Protein Science
         Biological Techniques
         Human Genetics
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig5_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs12033-024-01256-w/MediaObjects/12033_2024_1256_Fig6_HTML.png
      isPartOf:
         name:Molecular Biotechnology
         issn:
            1559-0305
            1073-6085
         type:
            Periodical
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Reza Didarian
            affiliation:
                  name:Ankara Yıldırım Beyazıt University
                  address:
                     name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hatice K. Ozbek
            affiliation:
                  name:Ankara Yıldırım Beyazıt University
                  address:
                     name:Metallurgical and Materials Engineering Department, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Veli C. Ozalp
            affiliation:
                  name:Atilim University
                  address:
                     name:Department of Medical Biology, School of Medicine, Atilim University, Ankara, Türkiye
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ozcan Erel
            affiliation:
                  name:Yıldırım Beyazıt University
                  address:
                     name:Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Türkiye
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Nimet Yildirim-Tirgil
            url:http://orcid.org/0000-0002-5973-8830
            affiliation:
                  name:Ankara Yıldırım Beyazıt University
                  address:
                     name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical"]:
      name:Molecular Biotechnology
      issn:
         1559-0305
         1073-6085
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Ankara Yıldırım Beyazıt University
      address:
         name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
         type:PostalAddress
      name:Ankara Yıldırım Beyazıt University
      address:
         name:Metallurgical and Materials Engineering Department, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
         type:PostalAddress
      name:Atilim University
      address:
         name:Department of Medical Biology, School of Medicine, Atilim University, Ankara, Türkiye
         type:PostalAddress
      name:Yıldırım Beyazıt University
      address:
         name:Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Türkiye
         type:PostalAddress
      name:Ankara Yıldırım Beyazıt University
      address:
         name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Reza Didarian
      affiliation:
            name:Ankara Yıldırım Beyazıt University
            address:
               name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
               type:PostalAddress
            type:Organization
      name:Hatice K. Ozbek
      affiliation:
            name:Ankara Yıldırım Beyazıt University
            address:
               name:Metallurgical and Materials Engineering Department, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
               type:PostalAddress
            type:Organization
      name:Veli C. Ozalp
      affiliation:
            name:Atilim University
            address:
               name:Department of Medical Biology, School of Medicine, Atilim University, Ankara, Türkiye
               type:PostalAddress
            type:Organization
      name:Ozcan Erel
      affiliation:
            name:Yıldırım Beyazıt University
            address:
               name:Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Türkiye
               type:PostalAddress
            type:Organization
      name:Nimet Yildirim-Tirgil
      url:http://orcid.org/0000-0002-5973-8830
      affiliation:
            name:Ankara Yıldırım Beyazıt University
            address:
               name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
      name:Metallurgical and Materials Engineering Department, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
      name:Department of Medical Biology, School of Medicine, Atilim University, Ankara, Türkiye
      name:Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Türkiye
      name:Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ankara, Türkiye
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(289)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.3s.

LINK . SPRINGER . COM {}