
NCBI . NLM . NIH . GOV {
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Title[redir]:
Differential Inhibitory Activities and Stabilisation of DNA Aptamers against the SARS Coronavirus Helicase - PMC
Description:
The helicase from severe acute respiratory syndrome coronavirus (SARSâCoV) possesses NTPase, duplex RNA/DNAâunwinding and RNAâcapping activities that are essential for viral replication and proliferation. Here, we have isolated DNA aptamers against ...
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Keywords {đ}
aptamers, helicase, aptamer, sarscov, doi, pubmed, google, scholar, gquadruplex, figure, pmc, dna, activity, article, structure, atpase, buffer, selection, free, binding, modified, gel, activities, inverted, nucleic, selected, thymidine, beads, sequence, nongquadruplex, inhibition, magnetic, biotin, rna, sequences, assay, acid, presence, min, electrophoresis, previously, library, values, inhibit, serum, purified, random, kcl, concentration, shown,
Topics {âď¸}
pcrÂŽâblunt iiâtopoÂŽ vector sarsâcovâhelicaseâbinding aptamer relied ďż˝terminal hexahistidine tag simple michaelisâmenten fit beadâbound sarsâcov helicase simple michaelisâmenten model qiaprep spin miniâprep bigâdye terminator kit niânta magnetic beads gave halfâmaximal stimulation pmc beta search conserved stemâloop structure ďż˝terminal metalâbinding domain ďż˝tagged sarsâcov helicase duplex rna/dnaâunwinding singleâstranded dna region sarsâcov helicase domain slowâmigrating multimeric band inhibit helicaseâunwinding activity excess sarsâcov helicase singleâstranded dna library sarsâcov helicase activities inhibit sarsâcov replication antiâsarsâcov drugs rnaâcapping enzymatic activity positive maximum peak apparent halfâstimulation values fretâbased fluorimetric assay purified sarsâcov helicase halfâmaximal stimulation sarsâcov genome license information pmcid single amino acid dna substrate contained publisher site pdf magnetic bead preparation metalâbinding domain helicaseâunwinding activity oligomeric nucleic acids stimulated atpase activity ďż˝quadruplexâforming aptamers ng1 high atpase turnover sarsâcov replication sarsâcov helicase stimulate atpase activity native polyacrylamide gel observe helicase activity ďż˝quadruplexâforming aptamers g5 singleâstranded aptamers dntp energy source
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