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We are analyzing https://link.springer.com/article/10.1007/s11011-025-01588-9.

Title:
Luteolin ameliorates ischemic/reperfusion injury by inhibiting ferroptosis | Metabolic Brain Disease
Description:
Ischaemic stroke is a large disease burden worldwide. Thrombolysis and thrombectomy are the main treatment methods for cerebral ischemia-reperfusion (I/R) injury. Luteolin, as a flavonoid compound, has an antagonistic effect on inflammation, oxidative stress, and tumorigenesis in disease. Therefore, the primary objective of this study is to determine the role of luteolin in cerebral I/R injury. Oxygen glucose deprivation/reoxygenation (OGD/R)-treated BV2 cells and a cerebral I/R rat model were established. Cell viability and death were verified using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and propidium iodide staining. The glutathione/oxidized glutathione (GSH/GSSG) ratio, superoxide dismutase (SOD) activity, and reactive oxygen species (ROS) and malondialdehyde (MDA) levels were determined using corresponding kits. Solute Carrier Family 7 Member 11 (SLC7A11), nuclear transcription factor erythroid 2-related factor 2 (NRF2), and glutathione peroxidase 4 (GPX4) levels were analyzed by western blotting. In addition, the infarct volume of brain tissues was examined by tetrazolium chloride (TTC) staining. Luteolin treatment significantly enhanced cell viability, decreased LDH release and intracellular ROS and MDA levels, and increased the GSH/GSSG ratio and SOD activity in OGD/R-treated BV2 cells. PI staining demonstrated that cell death was inhibited after luteolin treatment. Additionally, luteolin treatment significantly increased the SLC7A11, NRF2, and GPX4 protein levels. After treatment with ML385, an NRF2 inhibitor, the influence of luteolin on OGD/R-treated BV2 cells was reversed. Moreover, luteolin treatment significantly decreased the neurological score and infarct area in the brain tissues of cerebral I/R rats. Our research demonstrated that luteolin treatment inhibited ferroptosis by enhancing antioxidant functions through the NRF2 pathway, which provides a promising method for treating cerebral I/R injury.
Website Age:
28 years and 1 months (reg. 1997-05-29).

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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {🔍}

pubmed, article, google, scholar, cas, ferroptosis, luteolin, central, cerebral, injury, ischemiareperfusion, cell, nrf, hunan, liu, pathway, cancer, disease, research, brain, treatment, death, med, inhibiting, content, data, wanghua, oxidative, factor, rats, author, ischemia, privacy, cookies, publish, zeng, stroke, inflammation, role, access, axis, dis, biol, sci, activation, chinese, medicine, springer, information, search,

Topics {✒️}

tlr4/nf-κb/nlrp3 inflammasome pathway ting liu & wang-hua liu nuclear factor-e2‐related factor‐2 mir-378a-3p regulate ferroptosis p62-keap1-nrf2 pathway protects ros-rip1/rip3-exosome axis ogd/r-treated bv2 cells month download article/chapter gut microbiota-liver-brain axis wang-hua liu skin ischemia-reperfusion injury hepatic ischemia-reperfusion injury focal cerebral ischemia/reperfusion inhibiting ferroptosis-mediated neuroinflammation jin-xia li /r-induced renal injury inducible pd-l1 expression enhanced ros-boosted phototherapy improve anti-tumor immunity oxygen glucose deprivation/reoxygenation myocardial ischemia/reperfusion bdnf-mediated nrf2 activation nrf2/ho-1 signaling pathway kras-mutant lung cancer ischemia/reperfusion injuries vitro anti-inflammatory effects cystine transporter slc7a11/xct treating ferroptosis-related diseases cerebral ischemia-reperfusion cerebral ischemia reperfusion cerebral ischemia/reperfusion accepted manuscript version intestinal ischemia reperfusion inhibiting oxidative stress full article pdf slc7a11/gpx4 signaling pathway treated bv2 cells nrf2-mediated pathway xu-dong song anti-inflammatory effects nrf2/gpx4 axis activation brain glucose regulation acute ischemic stroke luteolin alleviates inflammation privacy choices/manage cookies activating pi3k/akt/nrf2 article li related subjects ampk/nrf2 pathway mitigating lipid peroxidation

Schema {🗺️}

WebPage:
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         headline:Luteolin ameliorates ischemic/reperfusion injury by inhibiting ferroptosis
         description:Ischaemic stroke is a large disease burden worldwide. Thrombolysis and thrombectomy are the main treatment methods for cerebral ischemia-reperfusion (I/R) injury. Luteolin, as a flavonoid compound, has an antagonistic effect on inflammation, oxidative stress, and tumorigenesis in disease. Therefore, the primary objective of this study is to determine the role of luteolin in cerebral I/R injury. Oxygen glucose deprivation/reoxygenation (OGD/R)-treated BV2 cells and a cerebral I/R rat model were established. Cell viability and death were verified using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and propidium iodide staining. The glutathione/oxidized glutathione (GSH/GSSG) ratio, superoxide dismutase (SOD) activity, and reactive oxygen species (ROS) and malondialdehyde (MDA) levels were determined using corresponding kits. Solute Carrier Family 7 Member 11 (SLC7A11), nuclear transcription factor erythroid 2-related factor 2 (NRF2), and glutathione peroxidase 4 (GPX4) levels were analyzed by western blotting. In addition, the infarct volume of brain tissues was examined by tetrazolium chloride (TTC) staining. Luteolin treatment significantly enhanced cell viability, decreased LDH release and intracellular ROS and MDA levels, and increased the GSH/GSSG ratio and SOD activity in OGD/R-treated BV2 cells. PI staining demonstrated that cell death was inhibited after luteolin treatment. Additionally, luteolin treatment significantly increased the SLC7A11, NRF2, and GPX4 protein levels. After treatment with ML385, an NRF2 inhibitor, the influence of luteolin on OGD/R-treated BV2 cells was reversed. Moreover, luteolin treatment significantly decreased the neurological score and infarct area in the brain tissues of cerebral I/R rats. Our research demonstrated that luteolin treatment inhibited ferroptosis by enhancing antioxidant functions through the NRF2 pathway, which provides a promising method for treating cerebral I/R injury.
         datePublished:2025-03-26T00:00:00Z
         dateModified:2025-03-26T00:00:00Z
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            Cerebral ischemia-reperfusion
            Luteolin
            NRF2
            Ferroptosis
            Neurosciences
            Neurology
            Metabolic Diseases
            Biochemistry
            general
            Oncology
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                        name:Key Laboratory of TCM Heart and Lung Syndrome Differentiation & Medicated Diet and Dietotherapy, Changsha, China
                        type:PostalAddress
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      headline:Luteolin ameliorates ischemic/reperfusion injury by inhibiting ferroptosis
      description:Ischaemic stroke is a large disease burden worldwide. Thrombolysis and thrombectomy are the main treatment methods for cerebral ischemia-reperfusion (I/R) injury. Luteolin, as a flavonoid compound, has an antagonistic effect on inflammation, oxidative stress, and tumorigenesis in disease. Therefore, the primary objective of this study is to determine the role of luteolin in cerebral I/R injury. Oxygen glucose deprivation/reoxygenation (OGD/R)-treated BV2 cells and a cerebral I/R rat model were established. Cell viability and death were verified using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and propidium iodide staining. The glutathione/oxidized glutathione (GSH/GSSG) ratio, superoxide dismutase (SOD) activity, and reactive oxygen species (ROS) and malondialdehyde (MDA) levels were determined using corresponding kits. Solute Carrier Family 7 Member 11 (SLC7A11), nuclear transcription factor erythroid 2-related factor 2 (NRF2), and glutathione peroxidase 4 (GPX4) levels were analyzed by western blotting. In addition, the infarct volume of brain tissues was examined by tetrazolium chloride (TTC) staining. Luteolin treatment significantly enhanced cell viability, decreased LDH release and intracellular ROS and MDA levels, and increased the GSH/GSSG ratio and SOD activity in OGD/R-treated BV2 cells. PI staining demonstrated that cell death was inhibited after luteolin treatment. Additionally, luteolin treatment significantly increased the SLC7A11, NRF2, and GPX4 protein levels. After treatment with ML385, an NRF2 inhibitor, the influence of luteolin on OGD/R-treated BV2 cells was reversed. Moreover, luteolin treatment significantly decreased the neurological score and infarct area in the brain tissues of cerebral I/R rats. Our research demonstrated that luteolin treatment inhibited ferroptosis by enhancing antioxidant functions through the NRF2 pathway, which provides a promising method for treating cerebral I/R injury.
      datePublished:2025-03-26T00:00:00Z
      dateModified:2025-03-26T00:00:00Z
      pageStart:1
      pageEnd:10
      sameAs:https://doi.org/10.1007/s11011-025-01588-9
      keywords:
         Cerebral ischemia-reperfusion
         Luteolin
         NRF2
         Ferroptosis
         Neurosciences
         Neurology
         Metabolic Diseases
         Biochemistry
         general
         Oncology
      image:
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         name:Springer US
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            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Hua Li
            url:http://orcid.org/0009-0008-6130-4813
            affiliation:
                  name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine
                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
                     type:PostalAddress
                  type:Organization
                  name:The Key Laboratory of Diagnostics of TCM in Hunan Province
                  address:
                     name:The Key Laboratory of Diagnostics of TCM in Hunan Province, Changsha, China
                     type:PostalAddress
                  type:Organization
                  name:Key Laboratory of TCM Heart and Lung Syndrome Differentiation & Medicated Diet and Dietotherapy
                  address:
                     name:Key Laboratory of TCM Heart and Lung Syndrome Differentiation & Medicated Diet and Dietotherapy, Changsha, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jin-xia Li
            url:http://orcid.org/0000-0002-0949-8365
            affiliation:
                  name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine
                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
                     type:PostalAddress
                  type:Organization
                  name:The Key Laboratory of Diagnostics of TCM in Hunan Province
                  address:
                     name:The Key Laboratory of Diagnostics of TCM in Hunan Province, Changsha, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yi-di Zeng
            url:http://orcid.org/0009-0005-0767-6921
            affiliation:
                  name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine
                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
                     type:PostalAddress
                  type:Organization
                  name:The Key Laboratory of Diagnostics of TCM in Hunan Province
                  address:
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                     type:PostalAddress
                  type:Organization
            type:Person
            name:Cai-xing Zheng
            url:http://orcid.org/0009-0008-3990-3318
            affiliation:
                  name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine
                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
                     type:PostalAddress
                  type:Organization
                  name:The Key Laboratory of Diagnostics of TCM in Hunan Province
                  address:
                     name:The Key Laboratory of Diagnostics of TCM in Hunan Province, Changsha, China
                     type:PostalAddress
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            name:Si-si Dai
            url:http://orcid.org/0009-0002-7143-9160
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                  name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine
                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
                     type:PostalAddress
                  type:Organization
                  name:The Key Laboratory of Diagnostics of TCM in Hunan Province
                  address:
                     name:The Key Laboratory of Diagnostics of TCM in Hunan Province, Changsha, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jian Yi
            url:http://orcid.org/0009-0009-6611-2818
            affiliation:
                  name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine
                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
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            type:Person
            name:Xu-dong Song
            url:http://orcid.org/0009-0005-1969-9127
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                  name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine
                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
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                  address:
                     name:Hunan University of Traditional Chinese Medicine Cultivates World First-class Disciplines of Traditional Chinese Medicine, Changsha, China
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                  name:Key Laboratory of TCM Heart and Lung Syndrome Differentiation & Medicated Diet and Dietotherapy
                  address:
                     name:Key Laboratory of TCM Heart and Lung Syndrome Differentiation & Medicated Diet and Dietotherapy, Changsha, China
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                  name:Hunan University of Traditional Chinese Medicine
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