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Title:
Crocin and Metformin suppress metastatic breast cancer progression via VEGF and MMP9 downregulations: in vitro and in vivo studies | Molecular and Cellular Biochemistry
Description:
Metastatic breast cancer remains a serious health concern and numerous investigations recommended medicinal plants as a complementary therapy. Crocin is one of the known anticancer bio-component. Recently, the inhibitory effect of metformin has been studied on the various aspects of cancer. However, no study reported their combination effects on metastatic breast cancer. In the present study, we have assessed their anti-metastatic effects on in vitro and in vivo breast cancer models. Using MTT assay, scratch, and adhesion tests, we have evaluated the cytotoxic, anti-invasive and anti-adhesion effects of crocin and metformin on 4T1 cell line, respectively. Their protective effects and MMP9 as well as VEGF protein expression levels (Western blotting) investigated in the 4T1 murine breast cancer model. Our results showed that both crocin and metformin reduced cell viability, delayed scratch healing and inhibited the cell adhesion, in vitro. While crocin alone restored the mice’s weight reduction, crocin, metformin, and their combination significantly reduced the tumor volume size and enhanced animal survival rate in murine breast cancer model, responses that were associated with VEGF and MMP9 down-regulation. These findings suggest that a combination of crocin and metformin could serve as a novel therapeutic approach to enhance the effectiveness of metastatic breast cancer therapy.
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pubmed, cancer, article, google, scholar, cas, breast, metformin, central, crocin, cell, metastasis, effects, cells, hoshyar, wang, university, abedini, human, biol, metastatic, molecular, mmp, study, oncol, med, birjand, department, research, anticancer, effect, tumor, saffron, int, zhang, privacy, cookies, content, data, information, progression, vegf, vitro, farahi, expression, model, clin, res, pathway, medical,
Topics {✒️}
cd19-chimeric antigen receptor-modified wnt/β-catenin pathway genes triple-negative breast cancer wnt/β-catenin signaling pathway month download article/chapter western blotting wnt/β-catenin pathway promotes cancer metastasis shahr-e-qods branch elham chamani metastatic breast cancer breast cancer cells vegf-vegfr1 signaling pathway breast cancer chemotherapy postmenopausal breast cancer full article pdf 4t1 cell line human gynecologic cancers de hoon jp privacy choices/manage cookies human gastric adenocarcinoma drives malignant progression global cancer statistics mohammad reza abedini anti-metastatic effects facilitates tumor metastasis author information authors molecular research center bmc cancer 13 cellular biochemistry aims related subjects metformin inhibits proliferation breast cancer melanoma cell metastasis anti-diabetic drug article farahi tumor volume size main extracellular matrix article molecular molecular genetics department human cancers anticancer bio-component michigan state university european economic area north indian women tjan-heijnen vc mda-mb-231 histone h1 structure h1–dna interaction bax/bcl-2 ratio
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headline:Crocin and Metformin suppress metastatic breast cancer progression via VEGF and MMP9 downregulations: in vitro and in vivo studies
description:Metastatic breast cancer remains a serious health concern and numerous investigations recommended medicinal plants as a complementary therapy. Crocin is one of the known anticancer bio-component. Recently, the inhibitory effect of metformin has been studied on the various aspects of cancer. However, no study reported their combination effects on metastatic breast cancer. In the present study, we have assessed their anti-metastatic effects on in vitro and in vivo breast cancer models. Using MTT assay, scratch, and adhesion tests, we have evaluated the cytotoxic, anti-invasive and anti-adhesion effects of crocin and metformin on 4T1 cell line, respectively. Their protective effects and MMP9 as well as VEGF protein expression levels (Western blotting) investigated in the 4T1 murine breast cancer model. Our results showed that both crocin and metformin reduced cell viability, delayed scratch healing and inhibited the cell adhesion, in vitro. While crocin alone restored the mice’s weight reduction, crocin, metformin, and their combination significantly reduced the tumor volume size and enhanced animal survival rate in murine breast cancer model, responses that were associated with VEGF and MMP9 down-regulation. These findings suggest that a combination of crocin and metformin could serve as a novel therapeutic approach to enhance the effectiveness of metastatic breast cancer therapy.
datePublished:2021-04-30T00:00:00Z
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Metastasis
Crocin
Metformin
Western Blotting
Biochemistry
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Cancer Research
Medical Biochemistry
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headline:Crocin and Metformin suppress metastatic breast cancer progression via VEGF and MMP9 downregulations: in vitro and in vivo studies
description:Metastatic breast cancer remains a serious health concern and numerous investigations recommended medicinal plants as a complementary therapy. Crocin is one of the known anticancer bio-component. Recently, the inhibitory effect of metformin has been studied on the various aspects of cancer. However, no study reported their combination effects on metastatic breast cancer. In the present study, we have assessed their anti-metastatic effects on in vitro and in vivo breast cancer models. Using MTT assay, scratch, and adhesion tests, we have evaluated the cytotoxic, anti-invasive and anti-adhesion effects of crocin and metformin on 4T1 cell line, respectively. Their protective effects and MMP9 as well as VEGF protein expression levels (Western blotting) investigated in the 4T1 murine breast cancer model. Our results showed that both crocin and metformin reduced cell viability, delayed scratch healing and inhibited the cell adhesion, in vitro. While crocin alone restored the mice’s weight reduction, crocin, metformin, and their combination significantly reduced the tumor volume size and enhanced animal survival rate in murine breast cancer model, responses that were associated with VEGF and MMP9 down-regulation. These findings suggest that a combination of crocin and metformin could serve as a novel therapeutic approach to enhance the effectiveness of metastatic breast cancer therapy.
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