We are analyzing https://link.springer.com/article/10.1007/s00262-020-02648-y.

Title:
PD-L1+ lung cancer stem cells modify the metastatic lymph-node immunomicroenvironment in nsclc patients | Cancer Immunology, Immunotherapy
Description:
Introduction Cancer stem cells (CSCs) are implicated in tumor initiation and development of metastasis. However, whether CSCs also affect the immune system is not fully understood. We investigated correlations between the PD-L1+ CSCs, changes in T-cell phenotype in metastatic and non-metastatic lymph nodes (LNs) and response to treatment. Methods LNs’ aspirates were obtained during the EBUS/TBNA procedure of 20 NSCLC patients at different stages of the disease. CSCs and T-cell characteristics were determined by flow cytometry. Results PD-L1+ CSCs positively correlated with the percentage of Tregs, PD-1+ CD4 T cells and Tim3+ CD4+ T cells, whereas PD-L1+ CSCs were negatively correlated with CD4+ T cells and CD28+ CD4+ T cells. The percentage of PD-L1+ CSCs was higher in patients with progressive disease (PD) as compared to patients with stable disease (SD) or partial response (PR). Among T cells, only PD-1+ CD4+ T cells and Tim3+ CD4+ T-cell frequencies were higher in patients with PD as compared to patients with SD or PR. Conclusion The frequency of PD-L1+ CSCs associates with an altered T-cell frequency and phenotype indicating that CSCs can affect the immune system. The higher percentage of PD-L1+ CSCs in patients with PD may confirm their resistance to conventional therapy, suggesting that CSCs may be an interesting target for immunotherapy.
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Keywords {🔍}

cells, cscs, cancer, pdl, patients, lns, metastatic, pubmed, article, percentage, lung, immune, cell, google, scholar, nonmetastatic, expression, tumor, correlated, frequency, tcell, tim, cas, stem, study, central, nsclc, tregs, confirmed, differences, flow, significant, analysis, phenotype, disease, samples, supplementary, fig, response, molecules, data, ebustbna, positively, lymphocyte, system, lymph, cytometry, higher, tme, performed,

Topics {✒️}

cd45−/cd184+/epcam+/cd133+/cd44+/cd90+ [14 anti-pd-1/pd-l1 monotherapy article download pdf pd-l1-negative cscs correlated van de ven metastatic lymph-node immunomicroenvironment anti-pd-l1/pd-1 therapies altered t-cell phenotype endobronchial ultrasound-guided biopsy t-cell subtypes frequencies thoracic oncology assembly routine ebus/tbna procedure altered t-cell frequency cd4 t-cell phenotype pd-l1+ cscs differs cd8+ t-cell phenotype tumor pd-l1 immunohistochemistry elevated foxp3/cd8 ratio cancer-immune set point pd-l1+ cscs associates anti-pd-l1 therapies lymph node metastasis metastatic lymph nodes pd-l1+ cancer cells pd-l1+ cscs correlates tumor antigen-specific cd4+ cd8+ t-cell frequency pd-l1+ cscs’ frequencies t-cell subsets lower cd8/treg ratio t-cell phenotyping standard chemo-radiotherapy depending related subjects t-cell phenotype high pd-l1 expression lung cancer diagnosis cancer stem cells privacy choices/manage cookies t-cell characteristics lung cancer progression cancer immunology–analysis pd-l1+ cscs suggest lung cancer aggressiveness lymph nodes lung cancer patients epithelial–mesenchymal transition epithelial-mesenchymal transition lung cancers lung cancer tme tumor cell heterogeneity

Questions {❓}

Costantini A, Grynovska M, Lucibello F, Moises J et al (2018) Immunotherapy: a new standard of care in thoracic malignancies?
Plaks V, Kong N, Werb Z (2015) The cancer stem cell niche: how essential is the niche in regulating stemness of tumor cells?

Schema {🗺️}

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