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Title:
A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature | Nature Structural & Molecular Biology
Description:
The evolutionarily conserved antisense long noncoding RNA asFGFR2 influences cell type–specific alternative-splicing patterns of FGFR2 by recruiting chromatin modifiers to the locus. Alternative pre-mRNA splicing is a highly cell type–specific process essential to generating protein diversity. However, the mechanisms responsible for the establishment and maintenance of heritable cell-specific alternative-splicing programs are poorly understood. Recent observations point to a role of histone modifications in the regulation of alternative splicing. Here we report a new mechanism of chromatin-mediated splicing control involving a long noncoding RNA (lncRNA). We have identified an evolutionarily conserved nuclear antisense lncRNA, generated from within the human FGFR2 locus, that promotes epithelial-specific alternative splicing of FGFR2. The lncRNA acts through recruitment of Polycomb-group proteins and the histone demethylase KDM2a to create a chromatin environment that impairs binding of a repressive chromatin-splicing adaptor complex important for mesenchymal-specific splicing. Our results uncover a new function for lncRNAs in the establishment and maintenance of cell-specific alternative splicing via modulation of chromatin signatures.
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nature portfolio permissions reprints privacy policy advertising intramural research program cancer research ptb-dependent spliced pkm2-e9 epithelial cell-type-specific regulators social media nature 456 nature 465 nature 479 nature 472 nature biotech research splicing-specific chromatin signature lys36-methylated histone h3 long con-coding rnas alternative pre-mrna splicing long noncoding rna cell-specific alternative splicing polycomb/trithorax epigenetic switch strand-specific qrt-pcr emt-markers e-cadherin strand-specific rt-pcr springerlink instant access alternative isoform regulation mcf10a-snail-er cells noncoding rna anril national cancer institute chromatin signature characteristic personal data x-fold change relative mesenchymal-specific splicing data protection tissue-specific splicing structural properties evolutionarily conserved elements jichi medical school histone modification complexes fgfr2 pre-mrna antisense exon iiib strand-specific rt alternative splicing complexity privacy mesenchymal-transition system cancer drug resistance n7-methylguanosine modification spliced exons iiib fgfr2 exon iiib
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headline:A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature
description:The evolutionarily conserved antisense long noncoding RNA asFGFR2 influences cell typeâspecific alternative-splicing patterns of FGFR2 by recruiting chromatin modifiers to the locus. Alternative pre-mRNA splicing is a highly cell typeâspecific process essential to generating protein diversity. However, the mechanisms responsible for the establishment and maintenance of heritable cell-specific alternative-splicing programs are poorly understood. Recent observations point to a role of histone modifications in the regulation of alternative splicing. Here we report a new mechanism of chromatin-mediated splicing control involving a long noncoding RNA (lncRNA). We have identified an evolutionarily conserved nuclear antisense lncRNA, generated from within the human FGFR2 locus, that promotes epithelial-specific alternative splicing of FGFR2. The lncRNA acts through recruitment of Polycomb-group proteins and the histone demethylase KDM2a to create a chromatin environment that impairs binding of a repressive chromatin-splicing adaptor complex important for mesenchymal-specific splicing. Our results uncover a new function for lncRNAs in the establishment and maintenance of cell-specific alternative splicing via modulation of chromatin signatures.
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Alternative splicing
Histone post-translational modifications
Long non-coding RNAs
Life Sciences
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Biochemistry
Protein Structure
Membrane Biology
Biological Microscopy
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description:The evolutionarily conserved antisense long noncoding RNA asFGFR2 influences cell typeâspecific alternative-splicing patterns of FGFR2 by recruiting chromatin modifiers to the locus. Alternative pre-mRNA splicing is a highly cell typeâspecific process essential to generating protein diversity. However, the mechanisms responsible for the establishment and maintenance of heritable cell-specific alternative-splicing programs are poorly understood. Recent observations point to a role of histone modifications in the regulation of alternative splicing. Here we report a new mechanism of chromatin-mediated splicing control involving a long noncoding RNA (lncRNA). We have identified an evolutionarily conserved nuclear antisense lncRNA, generated from within the human FGFR2 locus, that promotes epithelial-specific alternative splicing of FGFR2. The lncRNA acts through recruitment of Polycomb-group proteins and the histone demethylase KDM2a to create a chromatin environment that impairs binding of a repressive chromatin-splicing adaptor complex important for mesenchymal-specific splicing. Our results uncover a new function for lncRNAs in the establishment and maintenance of cell-specific alternative splicing via modulation of chromatin signatures.
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Histone post-translational modifications
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Protein Structure
Membrane Biology
Biological Microscopy
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