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We are analyzing https://www.nature.com/articles/nsmb.2230.

Title:
An alternative mode of microRNA target recognition | Nature Structural & Molecular Biology
Description:
MicroRNAs are thought to repress mRNA targets through perfect pairing with their seed region, but a sizeable number of miRNA interaction sites are orphans, without a perfect canonical miRNA partner. Now a large number of miRNAs are found to use an alternative binding mode that involves the bulging out of an unpaired mRNA nucleotide, leading to a functional mRNA-miRNA interaction. MicroRNAs (miRNAs) regulate mRNA targets through perfect pairing with their seed region (positions 2–7). Recently, a precise genome-wide map of miRNA interaction sites in mouse brain was generated by high-throughput sequencing and analysis of clusters of ~50-nucleotide mRNA tags cross-linked to Argonaute (Ago HITS-CLIP). By analyzing Ago HITS-CLIP
Website Age:
30 years and 10 months (reg. 1994-08-11).

Matching Content Categories {📚}

  • Education
  • Science
  • Animals & Wildlife

Content Management System {📝}

What CMS is nature.com built with?

Custom-built

No common CMS systems were detected on Nature.com, and no known web development framework was identified.

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What is the average monthly size of nature.com audience?

🌆 Monumental Traffic: 20M - 50M visitors per month


Based on our best estimate, this website will receive around 42,554,915 visitors per month in the current month.

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How Does Nature.com Make Money? {💸}


Display Ads {🎯}


The website utilizes display ads within its content to generate revenue. Check the next section for further revenue estimates.

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Direct Advertisers (10)
google.com, pmc.com, doceree.com, yourbow.com, audienciad.com, onlinemediasolutions.com, advibe.media, aps.amazon.com, getmediamx.com, onomagic.com

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How Much Does Nature.com Make? {💰}


Display Ads {🎯}

$536,300 per month
Our calculations suggest that Nature.com earns between $357,503 and $983,134 monthly online from display advertisements.

Keywords {🔍}

article, cas, google, scholar, nature, cell, microrna, rna, mol, micrornas, access, chi, nat, target, sites, biol, content, wook, darnell, argonaute, cookies, molecular, recognition, sung, hannon, mirnas, brain, hitsclip, struct, silencing, privacy, data, structural, biology, targets, pairing, seed, ago, binding, open, regulation, genes, health, institute, research, supplementary, information, alternative, mouse, interactions,

Topics {✒️}

nature portfolio permissions reprints privacy policy inosine editing author information authors research advertising social media ago clip-based imputation sung wook chi development precise genome-wide map piwi/mid domain protein microrna targeting specificity ago hits-clip facilitate age-specific functions nature 431 nature 433 nature 455 nature 456 nature 460 nature 466 nature 461 nature ago-mirna interactions regulate mrna targets seed-target recognition step springerlink instant access mirna interaction sites permissions risc slicer activity cold spring harbor personal data samsung advanced institute mirna-target interactions mir-302/367 microrna cluster samsung medical center genome-wide identification article chi data protection ago silencing complexes microrna target recognition privacy mice lacking mirna-1–2 microrna targeting code g-bulge sites competing financial interests microrna target sites microrna/target duplexes ago2 binding sites

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         headline:An alternative mode of microRNA target recognition
         description:MicroRNAs are thought to repress mRNA targets through perfect pairing with their seed region, but a sizeable number of miRNA interaction sites are orphans, without a perfect canonical miRNA partner. Now a large number of miRNAs are found to use an alternative binding mode that involves the bulging out of an unpaired mRNA nucleotide, leading to a functional mRNA-miRNA interaction. MicroRNAs (miRNAs) regulate mRNA targets through perfect pairing with their seed region (positions 2–7). Recently, a precise genome-wide map of miRNA interaction sites in mouse brain was generated by high-throughput sequencing and analysis of clusters of ~50-nucleotide mRNA tags cross-linked to Argonaute (Ago HITS-CLIP). By analyzing Ago HITS-CLIP 'orphan clusters'—Ago binding regions from HITS-CLIP that cannot be explained by canonical seed matches—we have now identified an alternative binding mode used by miRNAs. Specifically, G-bulge sites (positions 5–6) are often bound and regulated by miR-124 in brain. More generally, bulged sites comprise ≥15% of all Ago-miRNA interactions in mouse brain and are evolutionarily conserved. We call position 6 the 'pivot' nucleotide and suggest a model in which a transitional 'nucleation bulge' leads to functional bulge mRNA-miRNA interactions, expanding the number of potential miRNA regulatory sites.
         datePublished:2012-02-12T00:00:00Z
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      headline:An alternative mode of microRNA target recognition
      description:MicroRNAs are thought to repress mRNA targets through perfect pairing with their seed region, but a sizeable number of miRNA interaction sites are orphans, without a perfect canonical miRNA partner. Now a large number of miRNAs are found to use an alternative binding mode that involves the bulging out of an unpaired mRNA nucleotide, leading to a functional mRNA-miRNA interaction. MicroRNAs (miRNAs) regulate mRNA targets through perfect pairing with their seed region (positions 2–7). Recently, a precise genome-wide map of miRNA interaction sites in mouse brain was generated by high-throughput sequencing and analysis of clusters of ~50-nucleotide mRNA tags cross-linked to Argonaute (Ago HITS-CLIP). By analyzing Ago HITS-CLIP 'orphan clusters'—Ago binding regions from HITS-CLIP that cannot be explained by canonical seed matches—we have now identified an alternative binding mode used by miRNAs. Specifically, G-bulge sites (positions 5–6) are often bound and regulated by miR-124 in brain. More generally, bulged sites comprise ≥15% of all Ago-miRNA interactions in mouse brain and are evolutionarily conserved. We call position 6 the 'pivot' nucleotide and suggest a model in which a transitional 'nucleation bulge' leads to functional bulge mRNA-miRNA interactions, expanding the number of potential miRNA regulatory sites.
      datePublished:2012-02-12T00:00:00Z
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         miRNAs
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         Biological Microscopy
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