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Development, cytokine profile and function of human interleukin 17–producing helper T cells | Nature Immunology
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TH-17 cells are a distinct lineage of proinflammatory T helper cells that are essential for autoimmune disease. In mice, commitment to the TH-17 lineage is dependent on transforming growth factor-β and interleukin 6 (IL-6). Here we demonstrate that IL-23 and IL-1β induced the development of human TH-17 cells expressing IL-17A, IL-17F, IL-22, IL-26, interferon-γ, the chemokine CCL20 and transcription factor RORγt. In situ, TH-17 cells were identified by expression of the IL-23 receptor and the memory T cell marker CD45RO. Psoriatic skin lesions contained IL-23-producing dendritic cells and were enriched in the cytokines produced by human TH-17 cells that promote the production of antimicrobial peptides in human keratinocytes. Our data collectively indicate that human and mouse TH-17 cells require distinct factors during differentiation and that human TH-17 cells may regulate innate immunity in epithelial cells.
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headline:Development, cytokine profile and function of human interleukin 17âproducing helper T cells
description:TH-17 cells are a distinct lineage of proinflammatory T helper cells that are essential for autoimmune disease. In mice, commitment to the TH-17 lineage is dependent on transforming growth factor-β and interleukin 6 (IL-6). Here we demonstrate that IL-23 and IL-1β induced the development of human TH-17 cells expressing IL-17A, IL-17F, IL-22, IL-26, interferon-γ, the chemokine CCL20 and transcription factor RORγt. In situ, TH-17 cells were identified by expression of the IL-23 receptor and the memory T cell marker CD45RO. Psoriatic skin lesions contained IL-23-producing dendritic cells and were enriched in the cytokines produced by human TH-17 cells that promote the production of antimicrobial peptides in human keratinocytes. Our data collectively indicate that human and mouse TH-17 cells require distinct factors during differentiation and that human TH-17 cells may regulate innate immunity in epithelial cells.
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name:Edward P Bowman
affiliation:
name:Schering-Plough Biopharma (formerly DNAX Research)
address:
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
type:PostalAddress
type:Organization
name:Rene de Waal Malefyt
affiliation:
name:Schering-Plough Biopharma (formerly DNAX Research)
address:
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Present address: Entelos, Foster City, California 94404, USA.,
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Experimental Pathology and Pharmacology, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Experimental Pathology and Pharmacology, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Experimental Pathology and Pharmacology, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Experimental Pathology and Pharmacology, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Experimental Pathology and Pharmacology, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Laboratoire Cytokines et Inflammation EA 3806, Universite de Poitiers, Centre Hospitalier Universitaire de Poitiers, Pole Biologie Sante, Pineau, Poitiers, France
name:Laboratoire Cytokines et Inflammation EA 3806, Universite de Poitiers, Centre Hospitalier Universitaire de Poitiers, Pole Biologie Sante, Pineau, Poitiers, France
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Experimental Pathology and Pharmacology, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
name:Department of Discovery Research, Schering-Plough Biopharma (formerly DNAX Research), Palo Alto, USA
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