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Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma | Nature Genetics
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We have examined the structure and expression of the products associated with the t(2;13)(q35;q14) translocation associated with alveolar rhabdomyosarcoma. The chromosome 13 gene (FKHR) is identified as a member of the fork head domain family of transcription factors characterized by a conserved DNA binding motif. Polymerase chain reaction analysis demonstrates that a 5′ PAX3–3′ FKHR chimaeric transcript is expressed in all eight alveolar rhabdomyosarcomas investigated. Immunoprecipitation experiments detect the predicted fusion protein. These findings indicate that the t(2;13) generates a potentially tumorigenic fusion transcription factor consisting of intact PAX3 DNA binding domains, a truncated fork head DNA binding domain and C–terminal FKHR regions.
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nature portfolio permissions reprints privacy policy advertising nature genet social media dna-binding domain encoded nature 359 nature 363 nature hepatocyte-enriched transcription factor tissue-specific transcription factors development 118 rac1-mediated actin remodeling exhibits copy-number amplification pax3-foxo1 chromatin occupancy dna binding motif pax-foxo1 fusion proteins personal data springerlink instant access transcription factor family data protection fork head domain permissions chimaeric transcription factor human pax3 gene rna-binding protein privacy transcription factors characterized predicted fusion protein article galili journals search log minimal repression domain transcriptional activation domains jun proto-oncogene explore content alveolar rhabdomyosarcomas investigated subscription content telencephalon-restricted expression eukaryotic transcription factors mammalian transcription factors isolated human genes european economic area immunoprecipitation experiments detect institutional subscriptions read axial pattern formation klein-waardenburg syndrome waardenburg syndrome type höltingflorencia cidre-aranazmaximilian highly sensitive approach
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- The fork head domain: A novel DNA binding motif of eukaryotic transcription factors?
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