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Title:
The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs | Nature Communications
Description:
RNA-binding proteins (RBPs) exert a broad range of biological functions. To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. Combined analyses of these and recently published data sets define the core RBP repertoire conserved from yeast to man. Conserved RBPs harbour defined repetitive motifs within disordered regions, which display striking evolutionary expansion. Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed ‘enigmRBPs’), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. RNA-binding proteins (RBPs) are implicated in many biological functions. Here the authors expand the human and yeast RNA interactome identifying new and conserved RBPs, several of which with no prior function assigned to RNA biology or structural motifs known to mediate RNA-binding, and suggesting new roles of RNA as modulators of protein function.
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nature portfolio privacy policy advertising nature 494 nature 290 nature social media ovarian research middle upper tools 0/ reprints regulatory rna-binding proteins thermo fisher scientific complete mass-spectrometric map transcriptome-wide binding sites noncoding rna revolution-trashing double-stranded rna viruses enhance rna-binding specificity clear rna-binding specificity regular lc-ms runs mitochondrial cardiomyopathy/neuropathy syndrome cell type-specific expression research rna-binding mitochondrial enzyme proteome-wide protein quantification bifunctional methyltransferase--extensive moonlighting iclip--transcriptome-wide mapping humboldt-universität zu berlin rna-binding protein zc3h41 mammalian mrna-binding proteins photoactivatable-ribonucleoside-enhanced crosslinking rna-binding protein repertoire conserved rna-binding activity human rna-binding proteins rna-protein crosslink sites rna-binding orthologous proteins author correspondence defined rna-binding domains 2 ml screw-capped tubes tap-tagged yeast strains tap-tagged yeast proteins estimated energy content show dose-dependent enrichment disordered amino-acid residues permissions post-transcriptional gene regulation anti-gpf magnetic beads lc-ms/ms core mrna interactome mitochondrial hydroxysteroid dehydrogenase
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headline:The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs
description:RNA-binding proteins (RBPs) exert a broad range of biological functions. To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. Combined analyses of these and recently published data sets define the core RBP repertoire conserved from yeast to man. Conserved RBPs harbour defined repetitive motifs within disordered regions, which display striking evolutionary expansion. Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed âenigmRBPsâ), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. RNA-binding proteins (RBPs) are implicated in many biological functions. Here the authors expand the human and yeast RNA interactome identifying new and conserved RBPs, several of which with no prior function assigned to RNA biology or structural motifs known to mediate RNA-binding, and suggesting new roles of RNA as modulators of protein function.
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name:European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
name:Present address: University of Ljubljana, Faculty of Computer and Information Science, VeÄna pot 113, SI-1001 Ljubljana, Slovenia,
name:European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
name:European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
name:European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
name:European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
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