NATURE . COM {}

Matching Content Categories {📚}

• Social Networks
• Science
• Mobile Technology & AI

Content Management System {📝}

What CMS is nature.com built with?

Custom-built

No common CMS systems were detected on Nature.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of nature.com audience?

🌆 Monumental Traffic: 20M - 50M visitors per month


Based on our best estimate, this website will receive around 42,555,829 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Nature.com Make Money? {💸}

Display Ads {🎯}

Direct Advertisers (10)

Reseller Advertisers (38)

How Much Does Nature.com Make? {💰}

Display Ads {🎯}

$536,300 per month
According to our algorithms, Nature.com's monthly online income from display advertising ranges from $357,511 to $983,155.

Keywords {🔍}

nature, article, caspase, content, cell, enzymes, cookies, death, privacy, human, open, research, data, information, garciacalvo, access, advertising, differentiation, april, catalytic, properties, family, members, margarita, peterson, rasper, apoptosis, differ, january, author, permissions, optional, media, personal, parties, policy, journals, journal, original, paper, published, purification, erin, dita, john, vaillancourt, robert, zamboni, donald, nicholson,

Topics {✒️}

nature portfolio permissions reprints merck research laboratories privacy policy nature advertising general structure ac-xexd-amc social media author correspondence assemble α-acyloxyenamide electrophiles article garcia-calvo cell death differ personal data margarita garcia-calvo data protection permissions hela cell lysates privacy ac-tleu-asp journals search log explore content similar content european economic area ion exchange chromatography kcat/km values concerted domain motions article cite particulate danger signal red fluorescent probe invasive optical imaging accepting optional cookies caspase family enzymes involves folding mammalian inflammation manage preferences important biological implications https article luciferase-based inflammasome content robert zamboni & donald human origin journal publish apoptosis martin rights optional cookies //doi choices thornberry department essential cookies

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Purification and catalytic properties of human caspase family members
         description:Members of the caspase family of cysteine proteases are known to be key mediators of mammalian inflammation and apoptosis. To better understand the catalytic properties of these enzymes, and to facilitate the identification of selective inhibitors, we have systematically purified and biochemically characterized ten homologues of human origin (caspases 1–10). The method used for production of most of these enzymes involves folding of active enzymes from their constituent subunits which are expressed separately in E. coli, followed by ion exchange chromatography. In cases where it was not possible to use this method (caspase-6 and -10), the enzymes were instead expressed as soluble proteins in E. coli, and partially purified by ion exchange chromatography. Based on the optimal tetrapeptide recognition motif for each enzyme, substrates with the general structure Ac-XEXD-AMC were used to develop continuous fluorometric assays. In some cases, enzymes with virtually identical tetrapeptide specificities have kcat/Km values for fluorogenic substrates that differ by more than 1000-fold. Using these assays, we have investigated the effects of a variety of environmental factors (e.g. pH, NaCl, Ca2+) on the activities of these enzymes. Some of these variables have a profound effect on the rate of catalysis, a finding that may have important biological implications.
         datePublished:1999-04-09T00:00:00Z
         dateModified:1999-04-09T00:00:00Z
         pageStart:362
         pageEnd:369
         sameAs:https://doi.org/10.1038/sj.cdd.4400497
         keywords:
            caspase
            protease
            apoptosis
            inflammation
            Life Sciences
            general
            Biochemistry
            Cell Biology
            Stem Cells
            Apoptosis
            Cell Cycle Analysis
         image:
         isPartOf:
            name:Cell Death & Differentiation
            issn:
               1476-5403
               1350-9047
            volumeNumber:6
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Nature Publishing Group UK
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Margarita Garcia-Calvo
               affiliation:
                     name:Merck Research Laboratories
                     address:
                        name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Erin P Peterson
               affiliation:
                     name:Merck Research Laboratories
                     address:
                        name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Dita M Rasper
               affiliation:
                     name:Merck Research Laboratories
                     address:
                        name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:John P Vaillancourt
               affiliation:
                     name:Merck Research Laboratories
                     address:
                        name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Robert Zamboni
               affiliation:
                     name:Merck Research Laboratories
                     address:
                        name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Donald W Nicholson
               affiliation:
                     name:Merck Research Laboratories
                     address:
                        name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Nancy A Thornberry
               affiliation:
                     name:Merck Research Laboratories
                     address:
                        name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Purification and catalytic properties of human caspase family members
      description:Members of the caspase family of cysteine proteases are known to be key mediators of mammalian inflammation and apoptosis. To better understand the catalytic properties of these enzymes, and to facilitate the identification of selective inhibitors, we have systematically purified and biochemically characterized ten homologues of human origin (caspases 1–10). The method used for production of most of these enzymes involves folding of active enzymes from their constituent subunits which are expressed separately in E. coli, followed by ion exchange chromatography. In cases where it was not possible to use this method (caspase-6 and -10), the enzymes were instead expressed as soluble proteins in E. coli, and partially purified by ion exchange chromatography. Based on the optimal tetrapeptide recognition motif for each enzyme, substrates with the general structure Ac-XEXD-AMC were used to develop continuous fluorometric assays. In some cases, enzymes with virtually identical tetrapeptide specificities have kcat/Km values for fluorogenic substrates that differ by more than 1000-fold. Using these assays, we have investigated the effects of a variety of environmental factors (e.g. pH, NaCl, Ca2+) on the activities of these enzymes. Some of these variables have a profound effect on the rate of catalysis, a finding that may have important biological implications.
      datePublished:1999-04-09T00:00:00Z
      dateModified:1999-04-09T00:00:00Z
      pageStart:362
      pageEnd:369
      sameAs:https://doi.org/10.1038/sj.cdd.4400497
      keywords:
         caspase
         protease
         apoptosis
         inflammation
         Life Sciences
         general
         Biochemistry
         Cell Biology
         Stem Cells
         Apoptosis
         Cell Cycle Analysis
      image:
      isPartOf:
         name:Cell Death & Differentiation
         issn:
            1476-5403
            1350-9047
         volumeNumber:6
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Nature Publishing Group UK
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Margarita Garcia-Calvo
            affiliation:
                  name:Merck Research Laboratories
                  address:
                     name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Erin P Peterson
            affiliation:
                  name:Merck Research Laboratories
                  address:
                     name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Dita M Rasper
            affiliation:
                  name:Merck Research Laboratories
                  address:
                     name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:John P Vaillancourt
            affiliation:
                  name:Merck Research Laboratories
                  address:
                     name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Robert Zamboni
            affiliation:
                  name:Merck Research Laboratories
                  address:
                     name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Donald W Nicholson
            affiliation:
                  name:Merck Research Laboratories
                  address:
                     name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Nancy A Thornberry
            affiliation:
                  name:Merck Research Laboratories
                  address:
                     name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Cell Death & Differentiation
      issn:
         1476-5403
         1350-9047
      volumeNumber:6
Organization:
      name:Nature Publishing Group UK
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Merck Research Laboratories
      address:
         name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
         type:PostalAddress
      name:Merck Research Laboratories
      address:
         name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
         type:PostalAddress
      name:Merck Research Laboratories
      address:
         name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
         type:PostalAddress
      name:Merck Research Laboratories
      address:
         name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
         type:PostalAddress
      name:Merck Research Laboratories
      address:
         name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
         type:PostalAddress
      name:Merck Research Laboratories
      address:
         name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
         type:PostalAddress
      name:Merck Research Laboratories
      address:
         name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Margarita Garcia-Calvo
      affiliation:
            name:Merck Research Laboratories
            address:
               name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Erin P Peterson
      affiliation:
            name:Merck Research Laboratories
            address:
               name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
               type:PostalAddress
            type:Organization
      name:Dita M Rasper
      affiliation:
            name:Merck Research Laboratories
            address:
               name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
               type:PostalAddress
            type:Organization
      name:John P Vaillancourt
      affiliation:
            name:Merck Research Laboratories
            address:
               name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
               type:PostalAddress
            type:Organization
      name:Robert Zamboni
      affiliation:
            name:Merck Research Laboratories
            address:
               name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
               type:PostalAddress
            type:Organization
      name:Donald W Nicholson
      affiliation:
            name:Merck Research Laboratories
            address:
               name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
               type:PostalAddress
            type:Organization
      name:Nancy A Thornberry
      affiliation:
            name:Merck Research Laboratories
            address:
               name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
      name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
      name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
      name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
      name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
      name:Department of Enzymology, Merck Research Laboratories, Rahway, USA
      name:Department of Enzymology, Merck Research Laboratories, Rahway, USA

Social Networks {👍}(1)

• https://twitter.com/cddpress

External Links {🔗}(25)

• How much money does https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Margarita%20Garcia-Calvo generate?
• What's the revenue for https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Margarita%20Garcia-Calvo%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en?
• How much revenue does https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Erin%20P%20Peterson produce monthly?
• How much does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Erin%20P%20Peterson%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en net monthly?
• What are the total earnings of https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Dita%20M%20Rasper?
• How much income is https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Dita%20M%20Rasper%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en earning monthly?
• Learn how profitable https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=John%20P%20Vaillancourt is on a monthly basis
• How much does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22John%20P%20Vaillancourt%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en pull in monthly?
• How much profit is https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Robert%20Zamboni making per month?
• What is the earnings of https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Robert%20Zamboni%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en?
• What's the income of https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Donald%20W%20Nicholson?
• How much does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Donald%20W%20Nicholson%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en generate monthly?
• Check the income stats for https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Nancy%20A%20Thornberry
• How much does https://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Nancy%20A%20Thornberry%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en gross monthly?
• How much money does https://s100.copyright.com/AppDispatchServlet?title=Purification%20and%20catalytic%20properties%20of%20human%20caspase%20family%20members&author=Margarita%20Garcia-Calvo%20et%20al&contentID=10.1038%2Fsj.cdd.4400497©right=Macmillan%20Publishers%20Limited&publication=1350-9047&publicationDate=1999-04-09&publisherName=SpringerNature&orderBeanReset=true make?
• What's https://citation-needed.springer.com/v2/references/10.1038/sj.cdd.4400497?format=refman&flavour=citation's gross income?
• Monthly income for https://doi.org/10.1038/cddiscovery.2015.37
• How profitable is https://doi.org/10.1007/s00726-014-1855-3?
• https://doi.org/10.1038/ni.2919 income
• Profit of https://doi.org/10.1007/s12031-014-0325-4
• Profit of https://doi.org/10.1038/nmeth.2327
• What's https://www.protocols.io/'s gross income?
• How much revenue does https://www.natureindex.com/ produce monthly?
• Profit of http://www.naturechina.com
• How much cash flow does https://www.natureasia.com/ja-jp have monthly?

Analytics and Tracking {📊}

• Google Tag Manager

Libraries {📚}

• Prism.js
• Zoom.js

Emails and Hosting {✉️}

Mail Servers:

• mxa-002c5801.gslb.pphosted.com
• mxb-002c5801.gslb.pphosted.com

Name Servers:

• pdns1.ultradns.net
• pdns2.ultradns.net
• pdns3.ultradns.org
• pdns4.ultradns.org
• pdns5.ultradns.info
• pdns6.ultradns.co.uk