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Title:
Establishment in culture of pluripotential cells from mouse embryos | Nature
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Pluripotential cells are present in a mouse embryo until at least an early post-implantation stage, as shown by their ability to take part hi the formation of chimaeric animals1 and to form teratocarcinomas2. Until now it has not been possible to establish progressively growing cultures of these cells in vitro, and cell lines have only been obtained after teratocarcinoma formation in vivo. We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo. They have a normal karyotype.
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nature portfolio permissions reprints privacy policy advertising subscribe nature early development nature 292 nature social media early post-implantation stage personal data data protection springerlink instant access permissions privacy pluripotent cell lines expl cell res explore content subscription content european economic area institutional subscriptions read blood transfusion immunohaemat accepting optional cookies journals search log issue learn manage preferences article evans pluripotential cells cambridge university press content article purchase kaufman rights access article cite cell lines https 1038/292154a0 stem cells optional cookies mouse embryos choices essential cookies cookies skip tissue banking article journal publish institution buy evans department tissue culture //doi
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description:Pluripotential cells are present in a mouse embryo until at least an early post-implantation stage, as shown by their ability to take part hi the formation of chimaeric animals1 and to form teratocarcinomas2. Until now it has not been possible to establish progressively growing cultures of these cells in vitro, and cell lines have only been obtained after teratocarcinoma formation in vivo. We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo. They have a normal karyotype.
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